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Application of pseudomonas monteilii-containing microbial agent to purifying sulfurous gas

A technology of Pseudomonas monsonii and bacterial agents, applied in the field of environmental governance, can solve the problems of low organic chlorine treatment efficiency, uneven loading, and packing collapse, etc., and achieve the effects of short adaptation period, shortened start-up period, and less loss

Active Publication Date: 2018-09-14
DAJIANG ENVIRONMENT CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] In order to solve the problems in the prior art of volatile organic sulfur, low organic chlorine treatment efficiency, uneven loading of microorganisms on the surface of the mixed carrier, slow start-up, and collapse of fillers after long-term use, the present invention provides a strain of Pseudomonas monsonii (Pseudomonas monteilii), and apply the strain to the removal of organic sulfur-containing waste gas

Method used

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  • Application of pseudomonas monteilii-containing microbial agent to purifying sulfurous gas

Examples

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Effect test

Embodiment 1

[0044] Example 1 Application of a bacterial agent containing Pseudomonas monsonii in purifying sulfur-containing gas

[0045] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0046] (1) Fermentation broth preparation method

[0047] Pseudomonas monsonii LW-1 was cultured on a slant and then inserted into the fermentation medium, and the inoculum was expanded step by step according to 10% of the inoculum. Fermentation conditions: 35°C, ventilation rate 10L / min, dissolved oxygen > 2.0mg / L, tank Under the condition of pressure 0.03-0.10Mpa, the culture time is 55h;

[0048] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5g, NaCl0.5g, MgS...

Embodiment 2

[0056] Example 2 Application of a bacterial agent containing Pseudomonas monsonii in purifying sulfur-containing gas

[0057] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0058] (1) Fermentation broth preparation method

[0059] Pseudomonas monsonii LW-1 was cultured on a slant and then inserted into the liquid fermentation medium, and the culture was scaled up step by step according to the inoculum size of 25%. Fermentation conditions: 45°C, ventilation rate 100L / min, dissolved oxygen > 2.0mg / L , under the condition of tank pressure 0.03-0.10Mpa, the cultivation time is 45h;

[0060] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0...

Embodiment 3

[0067] The above-mentioned liquid bacterial agent is loaded on the ceramsite filler, and the concentration of Pseudomonas monsonii LW-1 bacteria in the filler is 9.0×10 8 CFU / g ( filler ) , used to treat the organic sulfur-containing waste gas produced by the pesticide wastewater storage tank, the gas flow rate is 5000m 3 / h, residence time 5.0min, temperature 55°C, total concentration of malathion, parathion, methyl parathion is 132.5mg / m 3 , the degradation efficiency of malathion, parathion and methyl parathion can reach 91.4%. Example 3 Application of a bacterial agent containing Pseudomonas monsonii in purifying sulfur-containing gas

[0068] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

...

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Abstract

The invention belongs to the field of environmental treatment and particularly relates to application of a pseudomonas monteilii-containing microbial agent to purifying sulfurous gas. Specifically, the pseudomonas monteilii-containing microbial agent is Pseudomonas monteilii LW-1 with a preservation number of CGMCC No. 13748. The pseudomonas monteilii-containing microbial agent is applied to processing organic sulfur-containing waste gas produced from a pesticide wastewater pool; during application, the gas flow is 100-5000 m3 / h, the retention time is 1.0-5.0 min, the temperature is 35-55 DEGC, the total concentration of malathion, parathion and methyl-parathion is 20-200 mg / m3, and the degrading efficiency of the malathion, the parathion and the methyl-parathion is up to higher than 90%.

Description

Technical field: [0001] The invention belongs to the field of environmental treatment, and in particular relates to the application of a bacterial agent containing Pseudomonas monteilii LW-1 in purifying volatile organic sulfur waste gas. Background technique: [0002] Wastewater from pesticide production contains a large amount of organosulfur pesticides and organochlorine pesticide intermediates, with complex components, high concentrations, and strong toxicity. Some organosulfur pesticides and organochlorine pesticide products or their intermediates, such as parathion, malathion, methyl parathion, chloral, phosphorus trichloride, etc., are volatile, and most of them are organic Sulfur and organochlorine pesticides have a pungent odor. [0003] Parathion (C 10 h 14 NO 5 PS) is a colorless oily liquid with a solubility in water of 24mg / L (25°C). Slightly soluble in petroleum ether and kerosene, miscible with most organic solvents, has garlic odor. Rapidly hydrolyzed i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/48C12R1/38
CPCB01D53/48B01D53/84B01D2257/306C12N1/20C12N1/205C12R2001/38Y02A50/20
Inventor 任仁朱利军柴春燕张徐祥
Owner DAJIANG ENVIRONMENT CORP
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