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Pseudomonas monteilii CY06 with efficient nitrogen reduction function and microecological preparation thereof, and application of microecological preparation

A technology of micro-ecological preparations and pseudomonas strains, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of large differences in nitrite efficiency and different action conditions, so as to improve the economic benefits of breeding and reduce Ammonia nitrogen and nitrite nitrogen, the effect of improving the quality of breeding

Active Publication Date: 2018-11-30
MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD +2
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Biological denitrification is not only safe and environmentally friendly, but also sustainable, but the efficiency of degrading nitrite varies greatly between different bacterial species and strains, and the action conditions are different

Method used

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  • Pseudomonas monteilii CY06 with efficient nitrogen reduction function and microecological preparation thereof, and application of microecological preparation
  • Pseudomonas monteilii CY06 with efficient nitrogen reduction function and microecological preparation thereof, and application of microecological preparation
  • Pseudomonas monteilii CY06 with efficient nitrogen reduction function and microecological preparation thereof, and application of microecological preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1, the screening of CY06 bacterial strain

[0026] 1. Screen the strains with blue halo and no hemolytic circle

[0027] Screening samples were collected from 100 water samples, fish samples, shrimp samples from fish ponds and shrimp ponds in Jiangsu, Guangdong and other places, as well as seawater samples and sand samples from Qingdao. In addition, the collected fish and shrimp samples were first sterilized with alcohol cotton, and the liver and intestines were dissected, ground in a small amount of sterile water, diluted and spread on a BTB solid culture plate, and placed in a 30°C incubator for cultivation At 20 hours, pick the colony with a blue halo around it, streak it on the LB plate, and purify twice. The single colony purified twice was planted on a fresh hemolysis plate with a toothpick, placed in a 30°C incubator and observed for 24h, 48h, and 72h to see if hemolysis circles appeared. Classified according to the ability to hemolyze blood cell aga...

Embodiment 2

[0053] Embodiment 2, the research of denitrification type

[0054] 1. Research on the nitrification characteristics of strain CY06

[0055] In the ultra-clean bench, the bacterial solution of the strain CY06 to be tested was inserted into 50ml of nitrification medium, and the final inoculation concentration was 5×10 5 cfu / mL, repeated 3 times; cultured in a shaker at 30°C, 180rpm, samples were taken to determine the content of ammonia nitrogen, nitrite and nitrate in the medium.

[0056] The result is as figure 1 As shown, the CY06 strain can rapidly remove NH 4 + , NH at 20h 4 + -N removal rate was 82.3%, and nitrite nitrogen was generated during the process.

[0057] 2. Study on denitrification characteristics of strain CY06

[0058] In the ultra-clean bench, the bacterial solution of the strain CY06 to be tested was inserted into 50ml denitrification medium, and the final inoculation concentration was 5×10 5 CFU / mL, repeated 3 times; cultured in a shaker at 30°C, 18...

Embodiment 3

[0063] Example 3, Whole Genome Sequencing Analysis of CY06 Strain

[0064] After whole genome sequencing analysis, it was found that the strain contained a 5,689,878bp chromosomal group, of which the G+C content was 61%, and contained 5110 coding genes; gene function analysis found that there was a nitrification and denitrification function in this strain Related enzymes, such as nitrate reductase, nitrite reductase, nitric oxide reductase, etc., and 20 enzymes related to nitrogen metabolism, such as glutamine synthetase, glutamate synthetase, nitrate monooxygenase, etc. protein.

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Abstract

The invention provides pseudomonas monteilii with an efficient nitrogen reduction function and a microecological preparation thereof, and application of the microecological preparation. The preservation number of the pseudomonas monteilii CY06 is CGMCC No.15249. Through experiments, it is shown that the degradation rate of the pseudomonas monteilii CY06 to nitrite nitrogen with the concentration being 2.5 mg / L is 96%. The pseudomonas monteilii CY06 provides a provenance for the aquatic water improved microecological preparation, harmful substances such as ammonia nitrogen and the nitrite nitrogen in water are reduced in pollution-free and residue-free modes, the cultivation quality is improved, cultivation economic benefits are increased, and the pseudomonas monteilii CY06 has popularization value.

Description

technical field [0001] The invention belongs to the technical field of aquatic micro-ecological preparations, and in particular relates to a high-efficiency nitrogen-reducing Pseudomonas monsonii strain CY06 and its micro-ecological preparation and application. Background technique [0002] China is the largest aquaculture country in the world. In 2016, the total amount of aquaculture aquatic products in my country reached 51.4239 million tons, accounting for more than 70% of the total aquaculture aquatic products in the world. Aquaculture has contributed to the supply of high-quality protein in my country and the development of agricultural economy. made a huge contribution. However, the water pollution caused by aquaculture cannot be ignored. The high-density farming mode and excessive feeding lead to high concentrations of ammonia nitrogen in the water body, especially nitrite nitrogen. Most of the ammonia nitrogen can be removed through the growth of algae in the water bo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F103/20
CPCC02F3/34C02F2103/20C12N1/20C12N1/205C12R2001/38
Inventor 何增国蔡亚丽马青山
Owner MARINE BIOMEDICAL RES INST OF QINGDAO CO LTD
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