Pseudomonas monteilii KY-05 and application thereof

A technology of Pseudomonas montseri and strains, applied in bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of unreported cellulose degradation and degradation function, high operating costs, secondary pollution, etc. The effect of increasing gas production and anaerobic reaction start-up speed and wide application prospects

Active Publication Date: 2015-05-20
TIANJIN CARING TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the hydrolysis of cellulose in sludge mainly relies on chemical and physical methods for sludge pretreatment, but this method has high operating costs and secondary pollution, and the use of cellulolytic bacteria to hydrolyze cellulose can greatly reduce to the operating cost, avoiding secondary pollution, and does not require specific equipment
[0004] Pseudomonas monsonii has been reported to have high application value in the prevention and control of tobacco mosaic virus, which can inhibit the infection of tobacco mosaic virus (TMV), but the bacteria in cellulose degradation Degradation function has not been reported yet

Method used

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  • Pseudomonas monteilii KY-05 and application thereof
  • Pseudomonas monteilii KY-05 and application thereof
  • Pseudomonas monteilii KY-05 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Screening and identification of Pseudomonas monteilii KY-05.

[0032] The experimental materials come from the anaerobic activated sludge samples of Tianjin Jizhuangzi Wastewater Treatment Plant.

[0033] Take sample diluent (dilution factor 10 -6 ) 0.1ml, spread on the screening medium plate, cultivate in a 30°C incubator, pick a single colony and inoculate it into the screening medium, intensify the culture for more than 48 hours, and repeat the streak purification 3 times to obtain the purified strain; after 16S rDNA And Biolog microbial classification and identification system identification, determined to be Pseudomonas monsonii KY-05, the phylogenetic tree is as follows figure 1 As shown, its 16S rDNA gene was amplified by PCR, and the sequencing result was shown as the sequence 1, namely:

[0034] 5'-TCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGCAGTAAGTTAATACCTTGCTGTTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGC...

Embodiment 2

[0036] Degradation test of Pseudomonas monteilii (Pseudomonas monteilii) KY-05 on macromolecular organic matter such as cellulose.

[0037] The processing object is the screening medium, and the operation mode is shaking culture in shake flasks. The specific implementation steps are as follows: Pseudomonas monsonii KY-05 described in Example 1 is inoculated to the enrichment medium, and cultured with shaking at 30° C. to the logarithmic growth phase (bacteria concentration is about 10 8 Individual / ml), inoculated in liquid screening medium with 3% inoculum size, cultured with shaking at 30°C for 1 day, and maintained dissolved oxygen at 1.0-3.0ppm; centrifuged to get supernatant as crude enzyme solution, according to DNS colorimetric method (GB / T 23881-2009) to determine the amount of reducing sugar produced by the strain degrading cellulose and other macromolecular complex organic matter. The results showed that the crude enzyme solution of the strain could produce an avera...

Embodiment 3

[0039] Synergistic experiment of Pseudomonas monteilii KY-05 on anaerobic digestion of municipal sludge.

[0040] The treatment object is anaerobic municipal sludge, and a 400mL simulated anaerobic reaction system is used for operation. The specific implementation steps are as follows: Pseudomonas monsonii KY-05 described in Example 1 is inoculated to the enrichment medium, and cultured with shaking at 30° C. to the logarithmic growth phase (bacteria concentration is about 10 8 Individuals / ml) were inoculated in a simulated anaerobic reaction system with a 0.2% inoculum amount, and operated under anaerobic conditions at a medium temperature (35±2° C.). Such as figure 2As shown, the gas production efficiency of the reaction system was monitored and compared with that of the non-inoculated group. The results showed that within 300 hours of reaction time, Staphylococcus pasteurianus KY-06 could make the mixed sludge gas production rate higher than that of the control group. Th...

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Abstract

The invention provides a Pseudomonas monteilii KY-05 with the collection number of CGMCC No.10172, which has cellulose degradation capacity. The Pseudomonas monteilii KY-05 has an enhancement effect on the biogas yield and anaerobic reaction start speed in the existing sludge anaerobic digestion technique, can be used for developing the microbial preparation for enhancing anaerobic digestion gas production efficiency, is gradually subjected to practical application and popularization in sludge plants, and has wide application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Pseudomonas monsonii KY-05 and its application. Background technique [0002] With the increase of urban domestic sewage discharge year by year, the sludge of urban sewage plants in my country has increased sharply. By the end of 2010, the total annual sludge production in my country (calculated with a moisture content of 80%) has reached about 30 million tons, and it is about 30 million tons per year. 10% growth rate. However, about 80% of dewatered sludge is directly landfilled or incinerated, causing secondary pollution to the environment and greatly reducing the environmental benefits of sewage treatment plants. Therefore, the issue of sludge stabilization, resource utilization, and harmless treatment and disposal has received increasing attention and attention. Sludge anaerobic digestion treatment is widely used because of its advantages such as low treatment cost, high pathoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P5/02C02F11/04C12R1/38
CPCC02F11/04C12P3/00C12P5/023C12N1/205C12R2001/38Y02E50/30Y02W10/20
Inventor 李玉庆张建刘范嘉陈剑
Owner TIANJIN CARING TECH DEV
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