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A bacterial agent comprising Pseudomonas monsonii

A technology of Pseudomonas montseri and bacterial agent, which is applied in the field of environmental management, can solve the problems of low organic chlorine treatment efficiency, uneven loading, collapse of packing, etc., and achieve the effects of short adaptation period, shortened start-up period, and less loss.

Inactive Publication Date: 2019-11-05
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] In order to solve the problems in the prior art of volatile organic sulfur, low organic chlorine treatment efficiency, uneven loading of microorganisms on the surface of the mixed carrier, slow start-up, and collapse of fillers after long-term use, the invention provides a strain of Pseudomonas monsonii (Pseudomonas monteilii), and apply the strain to remove waste gas containing organic sulfur and / or organic chlorine

Method used

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  • A bacterial agent comprising Pseudomonas monsonii

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Embodiment 1

[0044] Embodiment 1 A kind of bacterial agent for purifying waste gas containing organic sulfur

[0045] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0046] (1) Fermentation broth preparation method

[0047] Pseudomonas monsonii LW-1 was cultured on a slant and then inserted into the liquid fermentation medium, and the culture was scaled up step by step according to the inoculum size of 10%. Fermentation conditions: 35°C, ventilation rate 10L / min, dissolved oxygen>2.0mg / L, Under the condition of the tank pressure of 0.03-0.10Mpa, the culture time is 55h to obtain the fermentation liquid;

[0048] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ...

Embodiment 2

[0056] Embodiment 2 A kind of bacterial agent for purifying waste gas containing organic sulfur

[0057] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0058] (1) Fermentation broth preparation method

[0059]Pseudomonas monsonii LW-1 was cultured on a slant and then inserted into the liquid fermentation medium, and the culture was scaled up step by step according to the inoculum size of 25%. Fermentation conditions: 45°C, ventilation rate 100L / min, dissolved oxygen > 2.0mg / L , under the condition of tank pressure 0.03-0.10Mpa, culture time 45h to obtain fermentation liquid;

[0060] The composition of the slant medium is: glucose 0.5g, K 2 HPO 4 0.5g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 0.5g...

Embodiment 3

[0067] The above-mentioned liquid bacterial agent is loaded on the ceramsite filler, and the concentration of Pseudomonas monsonii LW-1 bacteria in the filler is 9.0×10 8 CFU / g ( filler ) , used to treat the organic sulfur-containing waste gas produced by the pesticide wastewater storage tank, the gas flow rate is 5000m 3 / h, residence time 5.0min, temperature 55°C, total concentration of malathion, parathion, methyl parathion is 132.5mg / m 3 , the degradation efficiency of malathion, parathion and methyl parathion can reach 91.4%. Embodiment 3 A kind of bacterial agent for purifying waste gas containing organic sulfur

[0068] A bacterial agent prepared by Pseudomonas monteilii (Pseudomonas monteilii) LW-1, which can be used to purify waste gas containing organic sulfur and solve the pollution problem of volatile organic sulfur produced in processes such as pesticide wastewater treatment, said The preparation method of bacterial agent is as follows:

[0069] (1) Fermentat...

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Abstract

The invention belongs to the field of environmental treatment and particularly relates to a microbial agent containing pseudomonas monteilii and application thereof. The pseudomonas monteilii is specifically pseudomonas monteilii LW-1; the preservation number of the pseudomonas monteilii is CGMCC No.13748. The microbial agent is used for treating organic sulfur-containing waste gas generated by apesticide wastewater storage tank; the gas flow is 100-5,000m<3> / h; the residence time is 1.0-5.0 minutes; the temperature is 35-55 DEG C; the total concentration of malathion, parathion and parathion-methyl is 20-200mg / m<3> or the concentration of trichloracetic aldehyde is 5-100mg / m<3>; the degradation rate of malathion, parathion and parathion-methyl can reach 90% or above; the degradation rateof trichloracetic aldehyde can reach75% or above.

Description

Technical field: [0001] The invention belongs to the field of environmental treatment, and in particular relates to a bacterial agent containing Pseudomonas monteilii LW-1 and its application in purifying volatile organic sulfur / organic chlorine waste gas. Background technique: [0002] Wastewater from pesticide production contains a large amount of organosulfur pesticides and organochlorine pesticide intermediates, with complex components, high concentrations, and strong toxicity. Some organosulfur pesticides and organochlorine pesticide products or their intermediates, such as parathion, malathion, methyl parathion, chloral, phosphorus trichloride, etc., are volatile, and most of them are organic Sulfur and organochlorine pesticides have a pungent odor. [0003] Parathion (C 10 h 14 NO 5 PS) is a colorless oily liquid with a solubility in water of 24mg / L (25°C). Slightly soluble in petroleum ether and kerosene, miscible with most organic solvents, has garlic odor. Ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/70B01D53/48C12R1/38
CPCB01D53/48B01D53/70B01D53/84C12N1/20Y02A50/20
Inventor 李琳薛松王彦杰杨凯雄刘俊新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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