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Phenol-degrading bacteria and method for preparing indigo by conversing indole

A phenol-degrading bacteria and phenol-degrading technology, applied in the field of bioengineering, can solve the problems of neglected advantages, single enzyme resources, limited research, etc., and achieve the effects of simple extraction steps, high product concentration, and low environmental pollution.

Active Publication Date: 2013-04-24
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creation of chemicals called indoles that are very effective at breaking down certain types of compounds found naturally or artificially produced on Earth' s surface (indicators). It also produces various different colors from these substances through specific enzyme reactions with other molecules like amino acids. These products have potential uses such as being used commercially in paints or coatings.

Problems solved by technology

Technologies described in this patents have been developed over time due to their potential benefits when compared against existing techniques like chemistry. However, there has always existed concerns about how safe it should be made up without relying solely upon naturally occurring organisms.

Method used

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  • Phenol-degrading bacteria and method for preparing indigo by conversing indole

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1, using the phenol-degrading bacteria Pseudomonas monteilii QM to biotransform indole to produce indigo, and investigate the influence of some parameters on the concentration of indigo.

[0025] The steps and parameters for the expanded cultivation and biotransformation of indole to produce indigo by the phenol-degrading bacteria Pseudomonas monteilii QM are as follows:

[0026] 1. Inoculate the phenol-degrading bacteria Pseudomonas monteilii QM strain on a solid plate LB medium (yeast powder 5g / L, peptone 10g / L, NaCl 5g / L, agar 15-20g / L, adjust the pH to 7.0-7.2), Cultivate statically at 37°C for 2 days, store in refrigerator at 4°C, and serve as activated seeds on the plate;

[0027] 2, use the plate activation seed of step 1 gained, be inoculated in the liquid M9 substratum (Na 2 HPO 4 12H 2 O 17.1g / L, KH 2 PO 4 3.0g / L, NH 4 Cl 1.0g / L, NaCl 0.5g / L, FeCl 3 0.00075g / L, 1g / L glucose, 2mmol / L MgSO4), shaking culture at 20-40°C, 50-300r / min for 12-16 hou...

Embodiment 2

[0035] Example 2, phenol-degrading bacteria Pseudomonas monteilii QM biotransforms indole to produce indigo.

[0036] Steps 1-4 are the same as in Example 1, and the dry weight concentration of the expanded cultured Pseudomonas monteilii QM bacteria obtained in step 3 is controlled to be 4.0 g / L.

[0037] In a 50mL Erlenmeyer flask, add 20mL of the expanded cultured Pseudomonas monteilii QM bacteria prepared in step 3, and 15 μL of the indole / acetone solution prepared in step 4, so that the concentration of indole in the reaction solution is 75 mg / L. , 300r / min shaking for 6 hours.

[0038] According to the measurement method described in Example 1, the indigo concentration recorded is 35.24 mg / L.

Embodiment 3

[0039] Example 3, phenol-degrading bacteria Pseudomonas monteilii QM biotransforms indole to produce indigo.

[0040] Steps 1-4 are the same as in Example 1, and the dry weight concentration of the expanded cultured Pseudomonas monteilii QM bacteria obtained in step 3 is controlled to be 8.0 g / L.

[0041] In a 50mL Erlenmeyer flask, add 20mL of the enlarged cultured Pseudomonas monteilii QM bacteria prepared in step 3, 20 μL of the indole / acetone solution prepared in step 4, so that the concentration of indole in the reaction solution is 100mg / L, at 40°C , 250r / min shaking reaction for 8 hours.

[0042] According to the measurement method described in Example 1, the indigo concentration recorded is 31.20 mg / L.

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Abstract

The invention belongs to the biology engineering technical field, and relates to a method for preparing indigo by conversing indole through phenol-degrading bacteria. The invention is characterized in that the phenol-degrading microbial strain capable of effectively catalyzing indole to synthesize indigo is employed, and is Pseudomonas monteilii QM, and the preservation number is CGMCC No.5054; when the biotransformation reaction is carried out, the cell dry weight concentration of the Pseudomonas monteilii QM bacterium in a reaction solution is 0.1-10g/L, the indole concentration is 25-200mg/L, under temperature of 20-40 DEG C, the shaking table rotating speed is controlled with 50-300r/min, and the oscillation reaction lasts for 1-8 hours. The condition of the biotransformation reaction is mild, the product concentration reaches as high as 42.5mg/L, the production period is short, the cost is low, the extraction step is simple, the production step is clean, and the method is suitable for industrial production and application.

Description

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Claims

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Application Information

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Owner DALIAN UNIV OF TECH
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