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Pseudomonas monteilii and application thereof

A technology of Pseudomonas monsonii and contaminated soil, applied in the direction of application, bacteria, and chemicals for biological control, etc., can solve the problems of corn seeding delay, incompleteness, and affecting the growth and development of corn seedlings, and achieve the goal of increasing income Effect

Active Publication Date: 2019-03-19
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuation of the drought in the Northeast in the spring of 2018 led to delays in corn planting, a drop in emergence rate and even dead seedlings, which directly affected the growth and development of corn seedlings
Maize is a crop that is moderately sensitive to soil salinization. Maize has a relatively low salt-alkali tolerance. When subjected to saline-alkali stress, it often fails to germinate normally, resulting in uneven, incomplete, and weak seedlings, which seriously affects the production of maize.

Method used

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  • Pseudomonas monteilii and application thereof
  • Pseudomonas monteilii and application thereof
  • Pseudomonas monteilii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Degradation efficiency of Pseudomonas monsonii to residual p-nitrophenol in soil

[0016] (1) p-Nitrophenol standard working curve is established:

[0017] Prepare p-nitrophenol standard solutions with concentrations of 200, 100, 50, 25, 12.5, 6.25, and 3.125 mg / l, take 400 μl of the prepared p-nitrophenol standard solution, and add 2 ml of 0.1 mol / l sodium hydroxide and 800 μl of methanol, after mixing, the absorbance value was measured at 400 nm, and drawn into a standard working curve. The standard working curve is y=0.0144x+0.054, and the correlation coefficient is 0.9977, which is used for the research on the degradation efficiency of the degrading bacteria on the residual p-nitrophenol in the soil.

[0018] (2) Preparation of bacterial solution

[0019] Take out the Pseudomonas monsonii stored at -20°C and dissolve it on ice; pipette 100 μl of the bacterial solution and add it to 20 ml liquid LB medium containing 50 μg / ml kan antibiotics for shakin...

experiment example 2

[0025] Experimental Example 2 Under drought stress, the growth-promoting effect of Pseudomonas monsonii on maize seedlings

[0026] 2.1 Indoor pot experiment

[0027]Mix field soil and peat soil 1:1, weigh 600g per pot. The corn seeds used are Jingke 968. Pick corn seeds with full grains, soak them in 10% NaClO solution for disinfection for 25 minutes, and rinse them with sterile water for 3-5 times. Pour tap water into the flowerpot filled with soil until the soil in the flowerpot is soaked, and record the amount of water required; take 10mL and 30mL of the prepared bacterial solution respectively, add the recorded amount of water and mix well, and then pour it into the filled soil in the flowerpot. Two sterilized corn seeds were sown in each flower pot, and divided into six experimental groups: ①drought, ②drought+10mL bacterial solution, ③dry+30mL bacterial solution, ④normal water supply, ⑤normal water supply+10mL bacterial ⑥Normal water supply + 30mL bacterial solution,...

experiment example 3

[0042] Experimental Example 3 Under the stress of sodium bicarbonate, the growth-promoting effect of Pseudomonas monsonii on corn seedlings

[0043] 3.1 Indoor potting experiment: Add 600g of prepared soil (field soil: peat soil = 1:1) to each flower pot, and add Na 2 CO 3 The aqueous solution simulates alkali stress, making the Na in the soil 2 CO 3 Concentrations were 0, 15, 25 and 40 mmol / kg. Add 15ml OD to the soil 600 =0.1 bacterium liquid, after corn seeding 30 days, treat that corn seedling grows to three leaves one heart stage, measure plant height, fresh weight, root length, dry weight and detect chlorophyll content (chlorophyll content measuring instrument), peroxidase content (healing Physiological and biochemical indicators such as creatol method), superoxide dismutase (nitroblue tetrazolium photochemical reduction method), proline content (ninhydrin method) and malondialdehyde content (thiobarbituric acid method) , with 15 biological replicates for each treat...

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Abstract

The invention discloses pseudomonas monteilii and application thereof and belongs to the technical field of agricultural fertilizers. The screened pseudomonas monteilii PN1 in the invention provides anovel microbial resource, has multiple biological functions, can achieve effects of degrading residual p-nitrophenol in soil and improving tolerance and stress resistance of maize seedlings to pesticide residues, drought, salt and alkali and other abiotic stresses, and has important guiding significances and application values for ensuring safe, green and high-efficiency production of corn and other crops.

Description

technical field [0001] The invention belongs to the technical field of agricultural fertilizers, in particular to rhizosphere growth-promoting bacteria Pseudomonas monteilii (Pseudomonas monteilii PN1). It can not only degrade the residual p-nitrophenol in the soil, but also be used in improving the drought tolerance and salt-alkali tolerance of corn seedlings. Background technique [0002] Corn is one of the three major food crops in the world, and its output has an important impact on the world's food supply. It is also the most important food, feed and economic crop in China. In the production process of corn, it is often subjected to abiotic stresses such as drought, salinity, cold damage and pesticide residues in the soil, which significantly reduces the yield of corn and brings serious economic losses to agricultural production. Among them, water plays a great role in the life activities of plants. After most plants suffer from drought stress, plant growth and develop...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A01N63/02A01P21/00C12R1/38
CPCA01N63/10B09C1/10C12N1/205C12R2001/38
Inventor 王岩任何军杨凤慕文洋杨正博
Owner JILIN UNIV
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