Composite microbial inoculum for degradation of livestock and poultry manure position and application of composite microbial inoculum

A technology of compound bacterial agent and livestock and poultry manure, which is applied in the field of livestock and poultry manure degradation compound bacterial agent, can solve problems such as slow speed, complex composition of livestock and poultry manure, and insufficient degradation of compound bacterial flora, so as to improve production efficiency and synergize The effect is obvious, and the effect of improving the economic benefit and ecological environmental benefit of the enterprise

Active Publication Date: 2020-06-26
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, my country's livestock and poultry waste treatment and resource utilization have achieved some results, but there are still many practical difficulties in fully realizing the energy and fertilizer utilization of livestock and poultry manure
At present, making organic fertilizer from livestock and poultry manure through aerobic fermentation is one of the main ways of resource utilization of livestock and poultry manure, but the composition of livestock and poultry manure is complex, including protein, fat, organic acid, cellulose, semi Composed of cellulose and inorganic salts and other components, currently relying on a single bacterial species or a simple combination of complex bacterial flora is not fully degraded and the speed is slow

Method used

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  • Composite microbial inoculum for degradation of livestock and poultry manure position and application of composite microbial inoculum
  • Composite microbial inoculum for degradation of livestock and poultry manure position and application of composite microbial inoculum
  • Composite microbial inoculum for degradation of livestock and poultry manure position and application of composite microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 single bacterium and compound bacterium produce lignocellulose degrading enzyme, starch and proteolytic enzyme ability

[0044] Enzyme activity kits (Suzhou Keming Biotechnology Co., Ltd.) were used to determine the enzyme activities related to the degradation of lignin, cellulose, starch and protein for both single and combined bacteria.

[0045] (1) β-glucosidase (β-GC) activity assay

[0046] β-glucosidase decomposes p-nitrobenzene-β-D-glucopyranoside to generate p-nitrobenzene, which has a maximum absorption peak at 400nm, and calculates β-glucosidase by measuring the rate of increase in absorbance vitality.

[0047] β-GC(nmol / min / ml)=(△A+0.0027)÷0.00543×V reverse total÷V sample÷T=61.39×(△A+0.0027)

[0048] (2) Determination of exo-β-1,4-glucanase (C1) activity using 3,5-dinitrosalicylic acid method to determine exo-β-1,4-glucanase catalytic microcrystals The content of reducing sugars produced by cellulose degradation.

[0049] C1(ug / min / ml)=1000×(...

Embodiment 2

[0074] The degradative effect of embodiment 2 single bacterium and complex bacterium to chicken manure

[0075] Weigh a certain amount of chicken manure and place it in a 250ml conical flask, absorb the equal amount of each bacterial solution into the conical flask, and mix evenly, put it in a 55 degree incubator for cultivation, after 15 days, dry the sample and weigh it to Chicken manure without inoculation was used as a control, and the degradation rate of chicken manure was calculated by weight loss method.

[0076] results and analysis

[0077] The effects of different bacterial flora combinations on the degradation of chicken manure were as follows: figure 1 shown. Among all single-bacteria treatments, No. 2 bacteria (B5) had the highest degradation rate of chicken manure, which was 17.66%. In the combination of the two bacteria, the combination of bacteria No. 2 and No. 3 (B5+NJAU-ND8) had the highest degradation rate of 24.12%. In the combination of 4 bacteria, the...

Embodiment 3

[0079] Embodiment 3 single bacterium and complex bacterium are to the degradation effect of pig manure

[0080] Chicken manure is replaced by pig manure, and all the other operating steps are the same as in Example 2.

[0081] results and analysis

[0082] The effects of different bacterial flora combinations on the degradation of pig manure are as follows: figure 2 shown. Among all the single-bacteria treatments, No. 2 bacteria (B5) had the highest degradation rate of pig manure, which was 11.37%. In the combination of the two bacteria, the combination of fungus B and No. 2 bacteria (NJAU-F4-11+B5) had the highest degradation rate of 23.80%. Among the 4 bacteria combinations, the combination of fungus B and bacteria No. 2, No. 3 and No. 4 (NJAU-F4-11+NJAU-ND8+NJAU-N45+B5) had the highest degradation rate of 34.11%. The degradation rate of the chicken manure by the optimal combination of four bacteria was significantly higher than that of two bacteria and single bacteria....

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Abstract

The invention provides a composite microbial inoculum for degradation of livestock and poultry manure position and an application of the composite microbial inoculum. The composite microbial inoculumconsists of thermomyces lanuginosus NJAU-F4-11 bacterial liquid, bacillus NJAU-N45 bacterial liquid, bacillus NJAU-ND8 bacterial liquid and bacillus B5 bacterial liquid. The application of the composite microbial inoculum is to accelerate compost fermentation. The application comprises the following steps: uniformly mixing livestock and poultry manure and auxiliary materials according to a certainC / N ratio, inoculating the mixed livestock and poultry manure and auxiliary materials with a liquid strain, piling the inoculated poultry manure and auxiliary materials into strip piles, and performing turning fermentation by adopting a strip pile type fermentation process. Compared with control groups without adding strains and control groups added with single strains, the addition of the composite microbial inoculum effectively promotes increase of the temperature of the piles at the initial stage of composting, promotes the piles to enter a cooling period in advance, and promotes improvement of the germination index and the like of the composting products.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and discloses a compound bacterial agent for degrading livestock and poultry manure and its application. Background technique [0002] At present, the national annual production of livestock and poultry manure is about 3.8 billion tons. Large-scale livestock and poultry manure is a huge organic matter and nutrient resource, but if it is not handled properly, it will have a serious negative impact on the environment and residents' lives. According to the 2010 Bulletin of the First National Survey of Pollution Sources, the chemical oxygen demand discharged by the livestock and poultry farming industry reached 12.6826 million tons, accounting for 96% of the total emissions from agricultural sources; the discharge of total nitrogen and total phosphorus was 1.0248 million tons and 160,400 tons, accounting for 38% and 56% of the total emissions from agricultural sources respectively. At pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/20C12R1/07C12R1/645
CPCC02F11/02C02F2103/20C05F3/00C12N1/14C12N1/20C05F17/20C05F11/00Y02W30/40
Inventor 沈其荣李荣何宙阳刘东阳沈标刘超
Owner NANJING AGRICULTURAL UNIVERSITY
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