Method and application for increasing oligomerization wood sugar content in black tea
A technology of xylooligosaccharides and black tea, which is applied in the field of microbial fermentation, can solve problems such as difficult to achieve, achieve the effect of improving quality, stabilizing quality, and overcoming quality instability
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Embodiment 1
[0025] Preparation of spore liquid of thermophilic cotton wool
[0026] Spread the thermophilic cotton wool on the PDA agar medium plate, culture at 50°C for 6-8 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend in sterile water, and then suspend the The solution was poured into a conical flask containing glass beads, shaken at 200r / min for 1h to make a spore suspension, counted on a cell counting board, and the concentration of spores was controlled at 1×10 6 ~5×10 6 individual / mL.
[0027] Preparation of spore liquid of Cystis coronis
[0028] Coronoidis spread on the PDA agar medium plate, cultured at 30°C for 6-8 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend them in sterile water, and then The suspension was poured into a conical flask containing glass beads, shaken on a shaker at 200r / min for 1h to make a spore suspension, counted on...
Embodiment 2
[0033] The preparation of the thermophilic cotton wool spore liquid and the Saccharomyces coronis spore liquid is the same as that in Example 1.
[0034] Specific implementation steps: Spray 300g of Maocha with water evenly until the water content reaches 20%, put the tea in a pressure-resistant sterilizer, heat up to 100°C, steam for 10 minutes, take out the Maocha, and evenly spray the diluent of thermophilic cotton wool spores , control the water content of the tea leaves to 26%, place them in an incubator with a temperature of 50°C and a relative humidity of 75%, and cultivate them for 2 days. Put the cultivated tea leaves in a pressure-resistant sterilizer, raise the temperature to 100°C, steam for 5 minutes, take out the raw tea, evenly spray the spore liquid of S. coronoidis, control the water content of the tea leaves to 27%, press the tea bricks, and place Cultivate in an incubator with a temperature of 28-30°C and a relative humidity of 75% for 5-7 days. After the cu...
Embodiment 3
[0038] The preparation of the thermophilic cotton wool spore liquid and the Saccharomyces coronis spore liquid is the same as that in Example 1.
[0039]Specific implementation steps: Spray 300g of Maocha with water evenly until the water content reaches 20%, put the tea in a pressure-resistant sterilizer, heat up to 100°C, steam for 10 minutes, take out the Maocha, and evenly spray the diluent of thermophilic cotton wool spores , control the water content of the tea leaves to 28%, place them in an incubator with a temperature of 50°C and a relative humidity of 75%, and cultivate them for 3 days. Put the tea after cultivation in a pressure-resistant sterilizer, raise the temperature to 100°C, steam for 5 minutes, take out the raw tea, evenly spray the spore liquid of Saccharomyces coronis, control the water content of the tea to 26%, press the tea brick, and place Cultivate in an incubator with a temperature of 28-30°C and a relative humidity of 75% for 5-7 days. After the cul...
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