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35results about How to "Color bright green" patented technology

Method for cultivation and production of paclitaxel from induction of taxus chinensis var. mairei axillary bud in vitro

The invention provides a method for extracting paclitaxel from induction of taxus chinensis var. mairei axillary bud germination in vitro and then cultivation and production. On the basis of a WPM (woody plant medium) minimal medium, the method uses (800-1000) mg / L of (NH4)2SO4 to replace 990 mg / L of K2SO4 to obtain the improved WPM minimal medium, and adding 0.01-4.5 mg / L of TIBA; pruning annualyoung shoots from a taxus chinensis var. mairei tree, cutting into small stem sections in 2.5cm, sterilizing, inoculating to the improved WPM minimal medium, and performing alternative culture, namely light culture and dark culture, in an artificial climate box for culturing temperature, wherein the temperature is 25+ / -1 DEG C in the daytime and is 20+ / -1 DEG C in the night, the illumination intensity is 1500lx, and relative humidity is 80%, thus rapidly and successfully inducing axillary buds based on the WPM plus 0.01-4.5 mg / LTIBA. By using the method, the industrialization of the taxus chinensis var. mairei axillary buds can be realized with large scale, short period, high reproduction rate and low cost. Buds can be induced around 15 days with 100% induction rate; and the taxus chinensis var. mairei axillary buds induced by the method is thick and short and has freshly green color. The content of paclitaxel in buds obtained by the method can reach 0.0042% in fifty days, which is higher than that of paclitaxel in natural buds.
Owner:JISHOU UNIVERSITY

Blood pressure-reducing willow leaf healthcare tea and method for making same

InactiveCN102715316AColor bright greenTreat hypertensionTea substituesInsomniaWater content
The invention belongs to the field of healthcare tea and particularly relates to willow leaf tea having a blood pressure-reducing effect. The method for making the willow leaf tea comprises the following steps of cleaning tender weeping willow shoots and leaves, which are collected before tomb-sweeping festival, by using fresh water, air-drying the tender shoots and leaves in a cool place to dewater and wither the tender shoots and leaves, stir-frying the shoots and leaves in an iron pan at the temperature of between 140 and 160 DEG C for 30 minutes so as to remove green, spreading the shoots and leaves on a surface plate, cooling for 2 to 3 hours, putting the shoots and leaves in the iron pan at the temperature of 80 to 100 DEG C for rolling and shaping, drying the shoots and leaves by using a drying machine at the temperature of between 60 and 65 DEG C for 4 to 5 hours until the water content of the shoots and leaves is less than 6 percent, and adding a proper quantity of auxiliary materials to obtain the blood pressure-reducing willow leaf healthcare tea. When taken by people for a long time, the blood pressure-reducing healthcare willow leaf tea has an effect of treating hypertension, and can alleviate the symptoms of dizziness, headache, insomnia, dreaminess, nocturia, dry mouth and the like.
Owner:山西省汾阳医院

Method for cultivation and production of paclitaxel from induction of taxus chinensis var. mairei axillary bud in vitro

The invention provides a method for extracting paclitaxel from induction of taxus chinensis var. mairei axillary bud germination in vitro and then cultivation and production. On the basis of a WPM (woody plant medium) minimal medium, the method uses (800-1000) mg / L of (NH4)2SO4 to replace 990 mg / L of K2SO4 to obtain the improved WPM minimal medium, and adding 0.01-4.5 mg / L of TIBA; pruning annual young shoots from a taxus chinensis var. mairei tree, cutting into small stem sections in 2.5cm, sterilizing, inoculating to the improved WPM minimal medium, and performing alternative culture, namely light culture and dark culture, in an artificial climate box for culturing temperature, wherein the temperature is 25+ / -1 DEG C in the daytime and is 20+ / -1 DEG C in the night, the illumination intensity is 1500lx, and relative humidity is 80%, thus rapidly and successfully inducing axillary buds based on the WPM plus 0.01-4.5 mg / LTIBA. By using the method, the industrialization of the taxus chinensis var. mairei axillary buds can be realized with large scale, short period, high reproduction rate and low cost. Buds can be induced around 15 days with 100% induction rate; and the taxus chinensis var. mairei axillary buds induced by the method is thick and short and has freshly green color. The content of paclitaxel in buds obtained by the method can reach 0.0042% in fifty days, which is higher than that of paclitaxel in natural buds.
Owner:JISHOU UNIVERSITY

A special nutrient solution for enteromorpha and its preparation method

The invention relates to a nutrient solution special for enteromorpha, and further relates to a preparing method of the nutrient solution special for enteromorpha. The preparing method includes the following steps that (1) seawater is sterilized; (2) nutrient elements are added into the seawater, and the mixture is mixed to be even; (3) antibacterial components are weighed in proportion; (4) lawn pennywort herbs, opuntia dillenii haws and onion in the antibacterial components are triturated or smashed, and the obtained liquid is taken; (5) herba houttuyniae, fructus cnidii, lophatherum gracile, dandelions, common selfheal fruit-spike, saxifrage and garlic are decocted, and the extract is taken; (6) shrubalthea bark, cocklebur fruits, resurrection lily rhizomes, rubus pluribracteatus roots, Indian epimeredi herbs, lotus nuts, folium mori, golden cypress bark and glutinous rice roots are ground, and ground powder is obtained; (7) the effective ingredients in the ground powder are extracted and concentrated, and extract is obtained; (8) the extract in a certain proportion is added into the seawater, and the mixture is mixed to be even. The nutrient solution and the preparing method have the advantages of being good in bacterium-free culturing effect and beneficial for growth of enteromorpha.
Owner:NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
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