A special nutrient solution for enteromorpha and its preparation method

A technology of nutrient solution and prolifera, applied in the field of algae cultivation, can solve problems such as poor effect and affect the growth of prolifera, and achieve the effect of promoting rapid growth, inhibiting the growth of bacteria or seaweed, and changing the irritating effect.

Inactive Publication Date: 2019-05-28
NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the aseptic culture of Enteromorpha mainly adopts disinfection method, physical method, antibiotic method, etc. However, the effect of aseptic culture is not good, and it will affect the growth of Enteromorpha to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method for Enteromorpha special nutrient solution, comprising the following steps:

[0027] (1) Put the seawater to be used into a high-pressure steam sterilizer, and sterilize it at 121°C for 20-30 minutes;

[0028] (2) Add 0.04g NaNO to every 1L of sterilized seawater 3 , 0.01g Na 2 HPO 4 12H 2 O, 0.02mgMnCl 2 4H 2 O, 0.26mg FeSO 4 ·7H 2 O, 3.5mgNa 2 EDTA, mix well and add 0.2mg VB 1 , 0.001mgVB 12 , 0.001mg biotin, mixed evenly;

[0029] (3) Weigh the following components by mass: 37 parts of coriander, 32 parts of cactus, 35 parts of onion, 29 parts of Houttuynia cordata, 21 parts of cnidium, 21 parts of light bamboo leaves, 22 parts of dandelion, Prunella vulgaris 21 parts, 20 parts of saxifrage, 11 parts of peppermint oil, 20 parts of garlic, 16 parts of hibiscus bark, 15 parts of cocklebur, 10 parts of kaempferia, 12 parts of papaya root, 10 parts of parsnip, 8 parts of lotus heart, 8 parts of mulberry leaves, 7 parts of cork bark, 5 par...

Embodiment 2

[0037] A preparation method for Enteromorpha special nutrient solution, comprising the following steps:

[0038] (1) Put the seawater to be used into a high-pressure steam sterilizer, and sterilize it at 121°C for 20-30 minutes;

[0039] (2) Add 0.042g NaNO to every 1L of sterilized seawater 3 , 0.01gNa 2 HPO 4 12H 2 O, 0.02mgMnCl 2 4H 2 O, 0.28mg FeSO 4 ·7H 2 O, 3.7mgNa 2 EDTA, mix well, then add 0.2mgVB 1 , 0.001mgVB 12 , 0.001mg biotin, mixed evenly;

[0040] (3) Weigh each component of the following mass parts: 32 parts of coriander, 33 parts of cactus, 30 parts of onion, 33 parts of Houttuynia cordata, 28 parts of Cnidium Fructus, 25 parts of light bamboo leaves, 27 parts of dandelion, Prunella vulgaris 18 parts, 17 parts of saxifrage, 9 parts of peppermint oil, 15 parts of garlic, 12 parts of hibiscus bark, 17 parts of cocklebur, 16 parts of kaempferia, 15 parts of papaya root, 14 parts of parsnip, 10 parts of lotus heart, 10 parts of mulberry leaves, 9 parts...

Embodiment 3

[0048] A preparation method for Enteromorpha special nutrient solution, comprising the following steps:

[0049] (1) Put the seawater to be used into a high-pressure steam sterilizer, and sterilize it at 121°C for 20-30 minutes;

[0050] (2) Add 0.05g NaNO to every 1L of sterilized seawater 3 , 0.01gNa 2 HPO 4 12H 2 O, 0.02mgMnCl 2 4H 2 O, 0.25mg FeSO 4 ·7H 2 O, 3.8mgNa 2 EDTA, mix well, then add 0.2mgVB 1 , 0.001mgVB 12 , 0.001mg biotin, mixed evenly;

[0051] (3) Weigh each component of the following mass parts: 34 parts of coriander, 30 parts of cactus, 33 parts of onion, 31 parts of Houttuynia cordata, 25 parts of Cnidium chinensis, 26 parts of light bamboo leaves, 25 parts of dandelion, Prunella vulgaris 25 parts, 21 parts of saxifrage, 12 parts of peppermint oil, 14 parts of garlic, 15 parts of hibiscus bark, 11 parts of cocklebur seed, 14 parts of kaempferia, 8 parts of Dawu bubble root, 15 parts of parsnip, 9 parts of lotus heart, 9 parts of mulberry leaves...

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Abstract

The invention relates to a nutrient solution special for enteromorpha, and further relates to a preparing method of the nutrient solution special for enteromorpha. The preparing method includes the following steps that (1) seawater is sterilized; (2) nutrient elements are added into the seawater, and the mixture is mixed to be even; (3) antibacterial components are weighed in proportion; (4) lawn pennywort herbs, opuntia dillenii haws and onion in the antibacterial components are triturated or smashed, and the obtained liquid is taken; (5) herba houttuyniae, fructus cnidii, lophatherum gracile, dandelions, common selfheal fruit-spike, saxifrage and garlic are decocted, and the extract is taken; (6) shrubalthea bark, cocklebur fruits, resurrection lily rhizomes, rubus pluribracteatus roots, Indian epimeredi herbs, lotus nuts, folium mori, golden cypress bark and glutinous rice roots are ground, and ground powder is obtained; (7) the effective ingredients in the ground powder are extracted and concentrated, and extract is obtained; (8) the extract in a certain proportion is added into the seawater, and the mixture is mixed to be even. The nutrient solution and the preparing method have the advantages of being good in bacterium-free culturing effect and beneficial for growth of enteromorpha.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a special nutrient solution for Enteromorpha, and also relates to a preparation method of the special nutrient solution for Enteromorpha. Background technique [0002] Enteromorpha is a genus of Chlorophyta Ulvaceae, there are about 40 species, and there are about 11 species in China. The common species in China are Enteromorpha chinensis, Enteromorpha flatus, and Enteromorpha barbata. Most species of seafood are widely distributed in oceans all over the world, and some species can also be found in brackish water or rivers. Enteromorpha often grows on rocks in the intertidal zone, on gravels in muddy beaches, and sometimes on the algal bodies of large seaweeds. Due to global climate change, water eutrophication and other reasons, the green tide of the large marine alga Enteromorpha broke out. Although Enteromorpha is non-toxic, like red tides, the massivel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
CPCA01G33/00Y02P60/20
Inventor 姜锡仁韩龙江李继业赵升刘一霆潘玉龙孙蓓蓓
Owner NORTH CHINA SEA ENVIRONMENTAL MONITORING CENT OF STATE OCEANIC ADMINISTATION
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