A kind of bacillus subtilis bacterial strain and application thereof

The technology of Bacillus subtilis and strain is applied to the Bacillus subtilis strain and its application field to achieve the effects of low nutrient loss rate, high hardness and broad application prospects

Active Publication Date: 2021-11-02
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For this reason, the embodiment of the present invention provides a kind of Bacillus subtilis bacterial strain and its application, to solve the rare problem in the prior art that the bacterial strain that obtains better effect in inhibiting Aspergillus carbon niger, Aspergillus niger, Penicillium chrysogenum is found

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  • A kind of bacillus subtilis bacterial strain and application thereof
  • A kind of bacillus subtilis bacterial strain and application thereof

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Embodiment 1

[0026] The present embodiment provides a method for preparing a high density Bacillus subtilis fermentation broth comprising the steps of:

[0027] 1) Seed strain was prepared: Bacillus subtilis NA plate medium on activation, a single colony was streaked on NA medium, the sealant 33 ℃ upside down incubator 24h, to give the activated strains, strains with shift the activated rings were transferred to strain in LB medium, at 41.5 ℃, 240r conditions / min, and 37h culture, seed bacteria solution obtained were stored at 1.5 ℃;

[0028] Preparation 2) high density fermentation liquid: Step 1) Preparation of seed bacteria was good, at 4.5% (V / V) was inoculated in the flask in a high density medium at a temperature of 41.5 ℃, 240r under / min condition, 36h culture, Bacillus subtilis CGMCC NO.16569 achieve viable count of 3.27 × 10 11 CFU / ml. Obtained bacterial suspension, the bacterial suspension after centrifugation was 10000r / min and the supernatant sterilized by filtration with...

Embodiment 2

[0030] The present embodiment provides a method for preparing a high density Bacillus subtilis fermentation broth comprising the steps of:

[0031] 1) Seed strain was prepared: Bacillus subtilis NA plate medium on activation, a single colony was streaked on NA medium, the sealant 33 ℃ upside down incubator 24h, to give the activated strains, strains with shift the activated rings were transferred to strain in LB medium, at 33 ℃, 180r conditions / min culturing 24h, resulting seed bacteria solution were stored at -1 deg.] C;

[0032] Preparation 2) high density fermentation liquid: Step 1) Preparation of seed bacteria was good, at 3% (V / V) was inoculated in the flask in a high density medium at a temperature of 33 ℃, 180r under / min conditions, culture 24h, Bacillus subtilis CGMCC NO.16569 achieve viable count of 3.41 × 10 11 CFU / ml. Obtained bacterial suspension, the bacterial suspension after centrifugation was 10000r / min and the supernatant sterilized by filtration with m...

Embodiment 3

[0034] The present embodiment provides a method for preparing a high density Bacillus subtilis fermentation broth comprising the steps of:

[0035] 1) Seed strain was prepared: Bacillus subtilis NA plate medium on activation, a single colony was streaked on NA medium, the sealant 33 ℃ upside down incubator 24h, to give the activated strains, strains with shift the activated rings were transferred to strain in LB medium, at 50 ℃, 300r conditions / min culturing 5Oh, resulting seed bacteria solution were stored at 4 ℃;

[0036] Preparation 2) high density fermentation liquid: Step 1) Preparation of seed bacteria was good, at 6% (V / V) was inoculated in the flask in a high density medium at a temperature of 50 ℃, 300r under / min conditions, culture 48h, Bacillus subtilis CGMCC NO.16569 achieve viable count of 3.60 × 10 11 CFU / ml. Obtained bacterial suspension, the bacterial suspension after centrifugation was 10000r / min and the supernatant sterilized by filtration with micropor...

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Abstract

The embodiment of the present invention discloses a strain of Bacillus subtilis and its application. The strain of Bacillus subtilis is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC NO.16569; the preservation time is: 2018 October 10. The embodiment of the present invention also provides a high-density fermentation broth of Bacillus subtilis containing the metabolites of the Bacillus subtilis strain. A kind of Bacillus subtilis bacterial strain that the embodiment of the present invention provides can carry out effective suppression to Aspergillus carbon niger, Aspergillus niger, Penicillium chrysogenum, and Aspergillus carbon niger, Aspergillus niger, Penicillium chrysogenum are the mold that grapes are easy to grow, therefore contain The Bacillus subtilis high-density fermentation broth of the metabolites of Bacillus subtilis can effectively prolong the storage period of grapes, and has the characteristics of high efficiency, safety and environmental protection, and has broad application prospects.

Description

Technical field [0001] TECHNICAL FIELD Example embodiments relate to microorganisms of the present invention, particularly relates to a strain of Bacillus subtilis and Its Applications. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a Bacillus sp., Having a biological oxidation preventing, antagonistic pathogenic microorganisms, improve the in vivo environment, enhanced immune function, produce more digestive enzymes and nutrients effect. B. subtilis growth process can produce subtilin, polymyxin, nystatin, gramicidin other pathogenic bacteria or pathogenic condition endogenous infection significantly inhibited the active substance. Intestinal among Bacillus subtilis as it can quickly consume free oxygen, the oxygen can be reduced gut, so that the benefit of a better survival of anaerobic bacteria suppressing portion. Studies have shown added to the chicken feed Bacillus subtilis can average chicken cecal Salmonella was significantly reduced by 58%, which ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/125
CPCA23B7/155A23V2002/00C12N1/20C12N1/205C12R2001/125A23V2200/10
Inventor 范俊峰张波陈敏宋佩泽周发牛琳茹
Owner BEIJING FORESTRY UNIVERSITY
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