Application of Penicillium ymf1.00864 Strain in Promoting Vegetable Growth

A Penicillium, strain technology, applied in the application, plant growth regulator, plant growth regulator and other directions, to achieve the effect of thick stem, leaf hypertrophy, and obvious growth promoting effect

Active Publication Date: 2019-08-23
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of Penicillium sp. YMF1.00864 to improve plant cold resistance and promote plant growth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Implementation 1: Cultivation of Penicillium sp. YMF1.00864(PE)

[0013] The culture condition of Penicillium sp. YMF1.00864 fermented liquid is: routinely activate Penicillium sp. YMF1.00864 in PDA solid medium for 3 days, under aseptic condition, 2cm 2 Bacteria of the same size were inoculated in a 500mL Erlenmeyer flask filled with 250mL PDB medium, at 28°C, 160r min -1 Shaker culture 7d. The media used above are:

[0014] PDA solid medium: 200g of peeled potatoes, cut into small pieces, boil for 30min, filter with 4 layers of gauze, add 20g of glucose to the filtrate, add water to 1L, add 1.5% (m / v) agar, extinguish at 121°C Bacteria for 20 minutes before use;

[0015] PDB liquid medium: 200g of peeled potatoes, cut into small pieces, boil for 30min, filter with 4 layers of gauze, add 20g of glucose to the filtrate, add water to 1L, sterilize at 121°C for 20 minutes before use.

Embodiment 2

[0016] Implementation 2: Preparation of medium for high-temperature fungal composting and cultivation of seedlings

[0017] Thermomyces duponti YM3-4 in PDA solid medium was routinely activated for 3 days, under sterile conditions, 2 cm 2 The size of the bacteria block was inoculated in a 500mL Erlenmeyer flask filled with 250mL PDB medium, and the culture conditions were 45°C, 160r·min- 1 Shake culture for 7 days, then mix the fermentation liquid with humus and compost (1g of humus is black loam, add 0.086mL of fermentation liquid, place in 45°C incubator for 15 days).

[0018] Cultivation of seedlings: use leafy vegetables (green vegetables or Chinese cabbage) and solanaceous fruits (tomatoes) or seeds of different varieties as materials. Among them: 1) Solanaceae tomato (Lycopersicum esculentum, purchased from Beijing Green Oriental Agricultural Technology Research Institute); 2) Brassicaceae plant Xiaoqingcai (Shanghai Siyueman, purchased from Suzhou Seed Company); 3) Chi...

Embodiment 3

[0019] Implementation 3 The promotion effect of Penicillium sp. YMF1.00864 (PE) on the growth of crop seedlings

[0020] When the seedlings grow to 4 leaves and 1 heart, take the seedlings with the same growth and transplant them into plastic cups filled with 300g of high-temperature fungal composted culture medium (humus: vermiculite: fine sand / 7:2:1, v / v / v) Middle (diameter is 9cm, height is 18cm), every strain seedling adds 40ml fixed root water. After 24 hours, 20ml of the PE fungal fermentation stock solution was applied to the roots of each seedling, and the PDB culture solution was used as a blank control. Ten replicates were set up for each experimental treatment. The average temperature during planting is 18.0°C-5.8°C.

[0021] The results are displayed in the following table:

[0022] Table 1: Effect of PE on leaf length, leaf width and stem diameter of tomato plants

[0023] PE experimental group C control group Increment Leaf length / cm 5.42...

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Abstract

The invention relates to the application of a penicillium YMF1.00864 strain in promoting vegetable growth, which belongs to the technical field of vegetable cultivation. Penicillium sp. YMF 1.00864 strain was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on April 1, 2013; the address of the preservation unit: Chinese Academy of Sciences, No. 1, Courtyard 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology; Deposit Number: CGMCC No.7407. Application of the Penicillium sp. YMF1.00864 strain of the present invention in promoting vegetable growth. Compared with the prior art, the beneficial effects of the present invention are: Penicillium sp. YMF1.00864 fermented liquid of the present invention can make the stem of vegetable thick and strong, and blade is more hypertrophy, stretches, and color is brighter green, to vegetable The growth-promoting effect is very obvious.

Description

technical field [0001] The invention relates to the application of Penicillium sp. YMF1.00864 strain in promoting vegetable growth, which belongs to the technical field of vegetable cultivation. Background technique [0002] my country is a large agricultural country with a large population, but the per capita arable land in our country is only 1.43 mu, which is less than half of the world average. Fertilizers play an important role in promoting grain production and harvest. However, in recent years, due to the unreasonable application of chemical fertilizers (usually inorganic compounds), soil acidification, compaction, fertility decline, crop quality deterioration, eutrophication of rivers and lakes, and environmental deterioration have occurred. Therefore, in the context of sustainable agricultural development, it is particularly important to find new fertilizer sources, especially biological fertilizer sources. [0003] Plant Growth Promoting bacteria are a class of mi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80
Inventor 牛雪梅张克勤桂小薇
Owner YUNNAN UNIV
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