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Method for preparing oligosaccharides with side chains by utilizing thermophilic filamentous fungus fermentation one-step method

A technology for filamentous fungi and Thermomyces sp

Inactive Publication Date: 2018-05-18
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no report on the method of producing xylooligosaccharides with side chains by one-step fermentation of thermophilic filamentous fungi, especially Thermomyces lanuginosus

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  • Method for preparing oligosaccharides with side chains by utilizing thermophilic filamentous fungus fermentation one-step method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Production of Xylooligosaccharides with Side Chains Using Corncob as a Substrate by Solid Fermentation

[0034] (1) Take the concentration as 1×10 7 Add 2ml of Thermomyces lanuginosus spore suspension per mL into 100mL sterile potato dextrose agar medium to activate the spores, culture at 50°C for 3 days on a shaker at 250 rpm to obtain a seed solution;

[0035] (2) Corncobs are crushed into particles with a particle diameter of 3-8mm, and the solid fermentation medium is prepared according to the formula of 300g / L of corncobs, 100g / L of soybean dregs, and 600mL / L of water, and mix for subsequent use;

[0036] (3) Insert the seed liquid prepared in step (1) into 10L solid fermentation medium at a volume ratio of 1:100, ventilate at a rate of 5L / min, and stir at a rate of 1 rpm at the same time, and continue aerobic fermentation 8 days;

[0037] (4) Sampling 40g per day during fermentation, adding 80mL H 2 O, use the DNS method and fluorescence-assisted suga...

Embodiment 2

[0038] Example 2 Production of Xylooligosaccharides with Side Chains Using Wheat Bran as Substrate Solid Fermentation

[0039] (1) Take the concentration as 1×10 8 1ml of Thermomyces lanuginosus spore suspension per mL was added to 200 mL of sterile potato dextrose agar medium to activate the spores, and cultured on a shaker at 50°C for 5 days at 200 rpm to obtain a seed solution;

[0040] (2) By the formula of wheat bran 300g / L, soybean dregs 50g / L, and water 650mL / L, a solid fermentation medium is prepared and mixed for subsequent use;

[0041] (3) Insert the seed liquid prepared in step (1) into 50L solid fermentation medium at a volume ratio of 1:100, ventilate at a rate of 5L / min, and stir at a rate of 2 rpm at the same time for continuous aerobic fermentation 5 days;

[0042] (4) Sampling 50g per day during fermentation, adding 100mL H 2 O, use the DNS method and fluorescence-assisted sugar electrophoresis (FACE) to detect the reducing sugar content in the medium, whe...

Embodiment 3

[0043] Example 3 Production of xylooligosaccharides with side chains using corn stalks as a substrate by solid fermentation

[0044] (1) Take the concentration as 1×10 8 Add 2 ml of the spore suspension of Thermomyces lanuginosus lanuginosus spores / mL into 200 mL of sterile potato dextrose agar medium to activate the spores, culture them on a shaker at 55°C for 3 days at 200 rpm, and prepare the seed solution;

[0045] (2) crush corn stalks into powders with a particle size of 2-5mm, prepare a solid fermentation medium according to the formula of corn stalk powder 250g / L, pea dregs 100g / L, and water 650mL / L, mix well and set aside;

[0046] (3) Insert the seed liquid prepared in step (1) into 10L solid fermentation medium at a volume ratio of 1:200, ventilate at a rate of 3L / min, and stir at a rate of 2 rpm at the same time for continuous aerobic fermentation 8 days;

[0047] (4) Sampling 40g per day during fermentation, adding 100mL H 2 O, use the DNS method and fluorescen...

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Abstract

The invention discloses a method for preparing oligosaccharides with side chains by utilizing a thermophilic filamentous fungus fermentation one-step method. The method is characterized in that the oligosaccharides with the side chains are prepared by utilizing a method for solid fermentation or liquid fermentation of thermomyces lanuginosus. An experiment verifies that through the thermophilic properties of the thermomyces lanuginosus, fermentation conditions are easy to control in the production process of the thermomyces lanuginosus; meanwhile, the thermomyces lanuginosus can utilize cheapcorn cobs, bran and other agricultural and sideline products to ferment and produce the oligosaccharides with the arabinose side chains; the invention disclosed by the invention is a sustainable production method, can reduce the raw material cost and the separation and purification cost and is an environment-friendly and efficient production process.

Description

technical field [0001] The invention relates to a method for preparing xylooligosaccharides with side chains by one-step fermentation of thermophilic filamentous fungi, in particular to a method for preparing xylooligosaccharides with side chains by one-step fermentation of Thermomyces lanuginosus. Background technique [0002] Functional oligosaccharides refer to oligosaccharides that cannot be absorbed and utilized when entering the human intestine, and have attracted widespread attention due to their unique physiological functions. Xylooligosaccharides are a kind of high value-added functional sugars. Since there is no enzyme system to degrade them in the human body, the residual rate in the intestinal tract is extremely high, so it can promote the proliferation of beneficial intestinal microorganisms. Studies have reported that daily intake of 2-10g xylooligosaccharides can reduce cholesterol and blood glucose levels, promote the proliferation of bifidobacteria in the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/04C12P19/00C12R1/645
CPCC12P19/00C12P19/04C12P19/12
Inventor 王禄山史泽露张丽丽陈冠军
Owner SHANDONG UNIV
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