48results about How to "Fermentation conditions are easy to control" patented technology

The invention relates to aspergillus niger M03 and applications of the aspergillus niger in preparation of Pu'er tea theine through microbial conversion. The aspergillus niger M03 strain capable of realizing bioconversion for preparing Pu'er tea theine is separated from Pu'er ripe tea, is preserved in the China General Microbiological Culture Collection Center (CGMCC), is assigned with the accession number of CGMCC No.10930. According to the aspergillus niger M03 and the applications, the aspergillus niger M03 strain is taken as a fermenting strain, the piling solid fermentation is adopted, and the sun-withering crude tea leaves of Yunnan large-leaf tea are adopted as the raw material; or liquid fermentation is adopted, catechin compounds and theanine are adopted as substrates, piling solid fermentation and liquid fermatneion are carried out, and thus the Pu'er tea theine compounds are prepared. The aspergillus niger M03 can be used for specifically preparing the Pu'er tea theine compounds, has the advantages that the environment is protected, the operation is simple, and the popularization is convenient, and thus the application prospect is very good.

The invention discloses a Paenibacillus polymyxa GY40 bacterial strain. The preservation number is CGMCC NO.14481. The invention also discloses a spore production fermentation culture method of the Paenibacillus polymyxa GY40 bacterial strain, and a method used for preparing bacterial powder and a wettable powder from the Paenibacillus polymyxa GY40 bacterial strain. Compared with terrestrial microorganisms, the Paenibacillus polymyxa GY40 bacterial strain possesses following advantages: salt resistance is excellent, growth temperature is low, and antibacterial effect is excellent. The spore production fermentation culture method is simple in formula; the main raw materials are agricultural product leftovers; the cost is low; the raw materials are widely available; bacterial strain growthspeed is high; fermentation temperature is low; fermentation time is short; fermentation conditions are convenient to control; fermentation spore yield is high; raw material cost is reduced; fermentation energy consumption is reduced; fermentation production efficiency is increased; the wettable powder possesses excellent preventing and treating effect on wheat scab and fusarium wilt of cucumber,and can be used in plantation of wheat and cucumber.

The invention discloses a solid-state fermentation device with a uniform material distribution function and a fermentation method. The solid-state fermentation device is divided into a working bin, a fermentation bin and a quantitative discharging bin with clear functions, and a standard fermentation system is constructed. Everyday quantitative discharging of the quantitative discharging bin is controlled, a working frame is arranged in the working bin, stirring devices and material distribution devices are uniformly arranged on the working frame, quantitative and uniform material distribution in the fermentation bin is realized, and materials in the fermentation bin are subjected to layered fermentation. The combination property of the parts is good, equipment maintenance, fermentation production and standard operation of a fermentation process are guaranteed with adoption of standard equipment, the automation degree is high, multiple groups of equipment can be combined for working without excessive increase of operators, the management expense and production cost are lower, batch production and batch operation are facilitated, and operation and control are easy, so that standardization of fermented products as well as storage and transport operation of the products is guaranteed.

The invention relates to a low temperature protease production strain, low temperature protease produced by the same and a gene of the enzyme. A strain of planococcussp. producing low temperature protease is separated from the southern Indian Ocean deep-sea sediment for the first time, the preservation number of the strain is CGMCC No.8088, the optimum growth temperature and the optimum enzyme production temperature are both 20DEG C, and the fermentation level protease yield can reach 280U/mL. The optimum operation temperature of the protease produced by the strain is 30-37DEG C, the optimum pH is 7.5-9.0, under the condition of 30DEG C, over 80% of the highest catalytic activity can be maintained, and in the range of 20-25DEG C, the protease still has high catalytic activity. The protease produced by the strain belongs to typical low temperature alkaline protease and has good catalytic activity at normal temperature, thus having high research value in food processing industry, cold water detergent development and other aspects.

The invention discloses a solid state fermentation device, system and fermentation method of movable material distribution. The fermentation device comprises functionally clear three parts, namely, a working bin, a fermentation bin and a quantitative discharge bin which establish the standardized fermentation system, wherein quantitative discharge is carried out every day through controlling the quantitative discharge bin; a movable rack is arranged in the working bin; a stirring device and a material distributing device are arranged on the movable rack; quantitative and uniform material distribution to the fermentation bin and layered fermentation of materials in the fermentation bin are achieved through the operation of the movable rack. According to the solid state fermentation device, system and fermentation method of movable material distribution, all the parts are good in modularity, and standardized operation of equipment maintenance, fermentation production and fermentation technology is guaranteed through the adoption of the standardized equipment; the fermentation device is high in degree of automation, multiple groups of equipment can be combined to work without adding too many operating staff, and the administration expense and production cost are relatively low, so that batch production and batch operation are facilitated, the device is easy to operate and control, and standardization of the fermented product, storage and transportation of the product is guaranteed.

The invention discloses an ocean-originated bacillus amyloliquefaciens GY30 strain. The preservation number of the ocean-originated bacillus amyloliquefaciens GY30 strain is CGMCC NO. 14480. The invention also discloses a spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain and a method for preparing bacterium power through the bacillus amyloliquefaciens GY30 strain. The bacillus amyloliquefaciens GY30 strain has the advantages of being high in salt tolerance, low in growth temperature, good in antibacterial effects, wide in antibacterial range and easy to culture, which do not exist in terrigenous microorganisms. The spore production fermentation medium composition and the culture method of the bacillus amyloliquefaciens GY30strain are simple, applied raw materials are mainly agricultural product leftovers, thereby being low in price, extensive in resources, high in strain growth speed, low in fermentation temperature, short in fermentation time, easy to control in fermentation conditions, high in spore fermentation yield and capable of reducing raw material costs and fermentation energy consumption and improving thefermentation production efficiency; bacterium powder prepared through the spore production fermentation medium composition and a culture method of the bacillus amyloliquefaciens GY30 strain is high ineffects on processing microorganism fertilizers or rice sheath blight disease preventing pesticides.

The invention discloses a method for preparing plectasins by saccharomyces cerevisiae. The method comprises the following steps: a, transforming and amplifying saccharomyces cerevisiae genes; b, building recombinant plasmids pYES2-CPS-Plec of the saccharomyces cerevisiae genes in yeast cells; c, transforming INVSC1 cells of saccharomyces cerevisiae genes by the recombinant plasmids pYES2-CPS-Plec; d, screening positive bacteria strains, and performing digestion and identification; e, cultivating transformed bacteria to obtain antimicrobial peptide saccharomyces cerevisiae genes; and f, identifying the activity of the antimicrobial peptide saccharomyces cerevisiae genes. The plectasins provided by the invention has an obvious bacteriostatic action on a saccharomyces cerevisiae expression system, and the antibacterial effect is stronger than that of the antibacterial effect before modification. The saccharomyces cerevisiae expression system provided by the invention has the following advantages of being better in antibacterial effect, fast in growth rate and high in yield, is likely to realize high-density culture and does not need adopt methanol induction; furthermore, a protein product for expressing secretion is likely to be separated and purified; moreover, the saccharomyces cerevisiae expression system has a plurality of posttranslational modification features, such as polypeptide folding, glycosylation, methylation, acetylation and the like; in addition, due to a clear genetic background, the saccharomyces cerevisiae expression system is likely to operate an expression vector.

The invention discloses a high-yield culture medium using dickeyazeae to produce broad-spectrum antibiotic zeamine and a culture method. 1L of the culture medium contains 1-30g of a carbon source, 0.5-5g of a nitrogen source, 0.05-5g of an inorganic salt of and 0.1-5g of an amino acid. The carbon source is one or a plurality of glucose, sucrose, mannitol, glycerin or fructose; the nitrogen source is one kind or a plurality of ammonium sulfate, sodium nitrate, ammonium nitrate or ammonium chloride; the inorganic salt is one or a plurality of ferrous sulfate, calcium chloride, manganese chloride, magnesium sulfate or potassium chloride; the amino acid is one or a plurality of aspartic acid, asparagine or proline. The yield of the antibiotic produced by use of the high-yield culture medium is 20-30 times more than the antibiotic produced by use of a basic culture medium, the high-yield culture medium is simple in components, simple in preparation, low in cost, and easy in purification and separation of the antibiotic, and the culture method has the advantages of simple operation and easy control of culture conditions, and is beneficial for large-scale preparation of the antibiotic.

The invention discloses a method for preparing oligosaccharides with side chains by utilizing a thermophilic filamentous fungus fermentation one-step method. The method is characterized in that the oligosaccharides with the side chains are prepared by utilizing a method for solid fermentation or liquid fermentation of thermomyces lanuginosus. An experiment verifies that through the thermophilic properties of the thermomyces lanuginosus, fermentation conditions are easy to control in the production process of the thermomyces lanuginosus; meanwhile, the thermomyces lanuginosus can utilize cheapcorn cobs, bran and other agricultural and sideline products to ferment and produce the oligosaccharides with the arabinose side chains; the invention disclosed by the invention is a sustainable production method, can reduce the raw material cost and the separation and purification cost and is an environment-friendly and efficient production process.

The invention belongs to the field of polylysine products, and particularly relates to a preparation method and a preparation device of polylysine. The preparation method of polylysine comprises the steps of seed selection, enlarged culture, fermentation preparation and filtration clarification. A polylysine product produced by the preparation method of polylysine is stable in passage and high inpolylysine production capacity; in addition, related experiments find that the polylysine product produced by the preparation method of polylysine is high in antibacterial ability, good in fresh-keeping effect and relatively high in safety; more importantly, compared with a traditional biological fermentation device, the preparation device for producing the polylysine product can easily control and adjust all related physical parameters in the fermentation condition, and a stirring assembly is arranged according to the electromagnetic induction driving principle, so that the problems that a rotating shaft is mounted on the sealing cover, the sealing cover is inconvenient to open, and the preparation device is easy to leak in the fermentation process due to the fact that the bottom end of the preparation device penetrates through the rotating shaft can be avoided.

The invention provides a method for producing a biological antibacterial feed with a garlic leaf as an additive. A protein feed for a rabbit is prepared by crushing corncob and sweet potato dregs into powder, adding a certain amount of a nitrogen source, inorganic salt and a solid accessory material, inoculating a certain amount of bacterial strains in proportion, carrying out fermentation at 55 to 65 DEG C under sealing conditions for 15 to 20 d, carrying out drying in the sun and adding 10.0 to 15.0% of garlic leaf powder. The bacterial strains comprise bacillus subtilis, rhizopus oryzae, Geobacillus stearothermophilus, pyrolytic cellulolytic bacteria and Thermoactinomyces, and an inoculation volume ratio is 1: 1: 1: 2: 2: 3, preferably. The fermented feed produced by using the method has high crude protein content, low crude fiber content, a great number of beneficial components and good palatability, can improve feed intake of commercial rabbits, enhance immunity, reduce incidence of disease and improve feeding quality, has a reasonably designed formula, is simple and convenient to prepare, has low cost and high benefits and is applicable to large-scale pollution-free rabbit culturing by vast peasant households and in culturing farms.

The invention discloses a ganoderma lucidum polysaccharide fermentation extraction method, a ganoderma lucidum polysaccharide composition and application thereof.The ganoderma lucidum polysaccharide fermentation extraction method comprises the steps that firstly, bacillus subtilis is utilized for fermentation, and the bacillus subtilis can generate various cellulase and protease, so that rapid disintegration of cell walls of sporocarp is promoted; a large number of intracellular active ingredients including ganoderan on cell walls and in cells are rapidly released; then, residues left after fermentation of the bacillus subtilis are reused, specifically, undegraded macromolecular polysaccharides in the residues are degraded through saccharomyces cerevisiae, the residues are secondarily utilized, and more kinds of micromolecular ganoderma lucidum polysaccharides are obtained through conversion; finally, secondary residues left after fermentation of the saccharomyces cerevisiae are subjected to final fermentation utilization, specifically, undegraded components in the secondary residues are fermented by adopting strains of lactic acid bacteria, lactic acid can be produced, and the effect of improving the moisturizing and skin tendering effects of fermentation products is achieved.