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Method for producing biological antibacterial feed with garlic leaf as additive

An additive, garlic leaf technology, applied in the field of microbial fermentation biological antibacterial feed and its preparation, to achieve the effects of low production cost, stable effect, and cheap process

Inactive Publication Date: 2015-11-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Garlic leaves contain protein, fat, carbohydrates, carotene, thiamine, riboflavin and other nutrients as well as calcium, phosphorus, iron, selenium and other mineral elements, but in the production process of garlic, a large amount of garlic leaves are discarded But not well developed and utilized (Wan Xiumei, Zhang Hongting, 2013)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Preparation of Rabbit Corn Cob and Sweet Potato Residue Mixed Fermented Feed Containing Natural Garlic Leaf Powder

[0038] (1) Strain rejuvenation: Scrape or pick a small amount of Bacillus subtilis, Rhizopus oryzae, Geobacillus stearothermophilus, Pyrolyticum cellulolyticum and pyrolytic actinomycetes under aseptic conditions and inoculate them on solid On the slant medium, culture Bacillus subtilis and Rhizopus oryzae in a constant temperature incubator at 30°C for 1 day; cultivate Geobacillus stearothermophilus, pyrolytic cellulolytic bacteria and high temperature actinomycetes in a constant temperature incubator at 60°C 2d;

[0039] Wherein said solid slant culture medium consists of:

[0040] Bacillus subtilis solid slant medium: tryptone 10.0g, glucose 1.0g, beef extract 5.0g, sodium chloride 5.0g, agar 15.0g. Adjust the pH to 7.2-7.4, add water to make up to 1L;

[0041] Rhizopus oryzae solid slant culture medium (PDA medium): 200.0 g of peeled p...

Embodiment 2

[0055] Example 2 Comparison of nutritional components before and after fermentation of corn cob and sweet potato dregs mixed feed

[0056] First crush corncobs and sweet potato dregs into a fine powder of more than 20 meshes, then mix 20.0% corn cob powder, 40.0% sweet potato dregs, 20.0% bean cake powder, 15.0% wheat bran, 2.0% urea, 2.0% ammonium sulfate, hydrogen phosphate Mix 1.0% dipotassium, 0.1% magnesium sulfate, and 0.01% ferric sulfate, add water and adjust to mix evenly so that the water content is 75%, and adjust the pH of the material to 7.0. Inoculate 10% of Bacillus subtilis, Rhizopus oryzae, Geobacillus stearothermophilus, Pyrolyticum cellulolyticum and thermoactinomycetes at a volume ratio of 2:1:3:4:4:6. Stir evenly, put it into a plastic bag, compact and seal it, and ferment at 60°C for 15 days. After the fermentation, the changes in the content of crude protein, starch, crude fat, sugar, crude fiber and other nutrients in the fermented feed and the control...

Embodiment 3

[0057] Example 3 The effect of feeding meat rabbits with corncob and sweet potato dregs mixed fermented feed containing natural garlic leaf powder

[0058] Select 40 healthy New Zealand meat rabbits about 2 months old, with normal growth and development, and randomly divide them into two groups, with 20 rabbits in each group, half male and half male. The experimental group was fed with corn cob and sweet potato dregs mixed fermented feed containing natural garlic leaf powder (adding 12.0% garlic leaf powder) prepared by the present invention, and the control group was fed with commercially available basal feed. The basal feed consisted of grass meal, cornmeal, wheat bran, bean cake, fish meal, bone meal, yeast and salt, the content of which was 35.0%, 20.0%, 25.0%, 15.0%, 1.5%, 2.0%, 1.0% and 0.5%, respectively. Raise for 2 months, feed once every morning and evening, and feed fresh grass at noon. Food and water were provided ad libitum. Clean the manure tray and water tank ...

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PUM

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Abstract

The invention provides a method for producing a biological antibacterial feed with a garlic leaf as an additive. A protein feed for a rabbit is prepared by crushing corncob and sweet potato dregs into powder, adding a certain amount of a nitrogen source, inorganic salt and a solid accessory material, inoculating a certain amount of bacterial strains in proportion, carrying out fermentation at 55 to 65 DEG C under sealing conditions for 15 to 20 d, carrying out drying in the sun and adding 10.0 to 15.0% of garlic leaf powder. The bacterial strains comprise bacillus subtilis, rhizopus oryzae, Geobacillus stearothermophilus, pyrolytic cellulolytic bacteria and Thermoactinomyces, and an inoculation volume ratio is 1: 1: 1: 2: 2: 3, preferably. The fermented feed produced by using the method has high crude protein content, low crude fiber content, a great number of beneficial components and good palatability, can improve feed intake of commercial rabbits, enhance immunity, reduce incidence of disease and improve feeding quality, has a reasonably designed formula, is simple and convenient to prepare, has low cost and high benefits and is applicable to large-scale pollution-free rabbit culturing by vast peasant households and in culturing farms.

Description

technical field [0001] The invention relates to a microbial fermented feed, in particular to a microbial fermented biological antibacterial feed containing natural garlic leaf powder and using corncobs and sweet potato dregs as main raw materials and a preparation method thereof. Background technique: [0002] In recent years, with the rapid development of animal husbandry, the demand for commercial feed is also increasing year by year. However, due to the deterioration of the ecological environment, there is a serious shortage of forage resources, which causes the price of commercial feed to rise, which further restricts the development of animal husbandry. Healthy and sustainable development. my country is a country with a large amount of food, and various agricultural wastes such as corncobs and sweet potato dregs are roughage resources with rich nutrition. Studies have confirmed that corn cobs contain 31.8% of crude fiber, 1.1% of crude protein, 0.6% of crude fat, 51.8%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16A23K1/00A23K1/18C12N1/20C12N1/14C12R1/125C12R1/845C12R1/01
Inventor 韩业君赵玉菲贾晓静彭小伟
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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