Production method of glucose oxidase for feeding

The technology of a glucose oxidase and production method, which is applied in the field of feed processing, can solve problems such as difficult control of fermentation conditions, and achieve the effects of easy control of fermentation conditions, high activity, and less contamination of miscellaneous bacteria

Inactive Publication Date: 2015-03-04
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a production method of glucose oxidase for feed, to solve the problem that the fermentation conditions of the existing common solid-state fermentation technology are difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The production method of glucose oxidase for feed is as follows:

[0032] The first step, strain activation: take out the slant of Penicillium specificum with high glucose oxidase production, streak and inoculate it on a PDA (potato dextrose agar medium, the same below) solid culture plate with a diameter of 10 cm, and cultivate it at 28°C for 72 hours , then spread and inoculate on a PDA solid culture plate with a diameter of 20 cm, and culture at 28°C for 48 hours to obtain activated Penicillium specificum.

[0033] Preparation of potato agar medium: 200g of peeled potatoes, cut into small pieces, add boiled juice for 30min, filter with gauze, add 15g of agar to the filtrate, continue heating and stirring until the agar dissolves, then add 20g of glucose, add distilled water to 1000mL, 115 Sterilize at ℃ for 20 minutes, spread the plates for later use;

[0034] The second step, primary seed culture: After the primary liquid seed medium is sterilized at 115°C for 20 m...

Embodiment 2

[0038] The production method of glucose oxidase for feed is as follows:

[0039] The first step, strain activation: take out the slant of the preserved high-yield Penicillium specificum of glucose oxidase, streak and inoculate it on a PDA solid culture plate, cultivate it at 32°C for 96 hours, and then spread and inoculate it on a PDA solid with a diameter of 20cm Culture plates were cultured at 32°C for 96 hours.

[0040] The preparation of the potato agar medium: cut 200 g of peeled potatoes into small pieces, add boiled juice for 30 min, filter through 8 layers of gauze, add 15 g of agar to the filtrate, continue heating and stirring until the agar is dissolved, then add 20 g of glucose, and add distilled water to 1000 mL , sterilized at 115°C for 20 minutes, spread on plates for later use;

[0041]The second step, primary seed culture: After the primary liquid seed medium is sterilized at 115°C for 20 minutes, transfer the activated Penicillium inoculum to the primary see...

Embodiment 3

[0049] The first step, strain activation: take out the slant of the preserved Penicillium specificum with high glucose oxidase production, streak and inoculate it on a PDA solid culture plate with a diameter of 10 cm, cultivate it at 30°C for 72 hours, and then spread and inoculate it on a PDA solid culture plate with a diameter of 10 cm. A 20cm PDA solid culture plate was cultured at 30°C for 72h.

[0050] The preparation of the potato agar medium: cut 200 g of peeled potatoes into small pieces, add boiled juice for 30 min, filter through 8 layers of gauze, add 15 g of agar to the filtrate, continue heating and stirring until the agar is dissolved, then add 20 g of glucose, and add distilled water to 1000 mL , sterilized at 115°C for 20 minutes, spread on plates for later use;

[0051] The second step, primary seed culture: After the primary liquid seed medium is sterilized at 115°C for 20 minutes, transfer the activated Penicillium inoculum to the primary seed medium, and th...

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Abstract

The invention relates to a production method of glucose oxidase for feeding, and aims at solving the problem of an existing common solid state fermentation technology that the fermentation conditions are difficult to control. The production method of the glucose oxidase for feeding comprises the following steps: culturing activated penicillium notatum for at least one time to obtain penicillium notatum seeds; fermenting the penicillium notatum seeds by adopting a fluidized bed solid state fermentation method; after the fermentation, drying a solid culture medium, a fermentation product and a fermentation strain in a fluidized bed solid state fermentation tank; and then crushing the product to obtain a glucose oxidase finished product. The production method is easy to control, the product is not easily polluted by infectious microbes, and three wastes are not generated so that no pollution to the environment is caused; and after the fermentation, the fermentation product, namely the glucose oxidase, does not need to be purified, and rice dregs and / or rice bran, penicillium notatum strains and the product glucose oxidase can be directly dried to prepare the glucose oxidase for feeding. The production method has a simple process, and the biological cycle utilization of the rice dregs and / or rice bran can be promoted.

Description

technical field [0001] The invention belongs to the field of feed processing, and in particular relates to a production method of glucose oxidase for feed. Background technique [0002] Glucose oxidase is widely distributed in animals, plants and microorganisms. However, the amount of glucose oxidase in animals and plants is not abundant, so the extraction of glucose oxidase from animals and plants has certain limitations. Under certain conditions, the mold has a strong ability to produce glucose oxidase, which is convenient for large-scale production of glucose oxidase. The main fungi that produce glucose oxidase on a large scale are from Aspergillus niger and Penicillium. Enzyme production methods can be divided into extraction, fermentation and chemical synthesis. Fermentation has been the main method of enzyme production since the 1950s. [0003] The existing industrial production method of glucose oxidase for feed generally adopts liquid submerged fermentation techn...

Claims

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Application Information

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IPC IPC(8): A23K1/165
Inventor 周慧易翠平
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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