Multi-stage liquid submerged fermentation method of Ganoderma lucidum
A liquid deep fermentation, multi-stage technology, applied in the directions of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems affecting the quality stability of Ganoderma lucidum triterpenes, long production cycle of Ganoderma lucidum triterpenes, etc., and achieve the fermentation process. Simple and easy to implement, easy to control fermentation conditions, fast effect
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Embodiment 1
[0039] 3L fermenter multi-stage fermentation production of Ganoderma lucidum:
[0040] The pH value of the fermentation broth was controlled by feeding hydrochloric acid (2moL / L) or sodium hydroxide (2moL / L) during the fermentation process.
[0041] (1) The first stage of fermentation (0-26h), control the fermentation pH to the original pH of the medium (about 4.8-5.0), the temperature is 26°C, and the stirring speed is 80r / min; (2) The second stage of fermentation (26-36h ), the fermentation pH is controlled to be 4.0, the temperature is 26°C, and the stirring speed is 80r / min; (3) the third stage of fermentation (36-81h), the fermentation pH is controlled to be 4.5, the temperature is 26°C, and the stirring speed is 80r / min; ( 4) In the fourth stage of fermentation (81h-168h), the fermentation pH is controlled to be 5.0, the temperature is 26°C, the stirring speed is 80r / min, and the fermentation is completed until 168h.
[0042] Extraction of Ganoderma lucidum triterpenes:...
Embodiment 2
[0053] During the fermentation process, the pH value of the fermentation broth was controlled by feeding hydrochloric acid (2moL / L) or sodium hydroxide (2moL / L).
[0054] 50L fermenter multi-stage fermentation production of Ganoderma lucidum triterpenes:
[0055] (1) The first stage of fermentation (0-26h), control the fermentation pH to be natural, the temperature is 26 ° C, and the stirring speed is 80r / min; (2) The second stage of fermentation (26-36h), control the fermentation pH to 4.0, the temperature (3) The third stage of fermentation (36-81h), control the fermentation pH to 4.5, the temperature is 26°C, and the stirring speed is 80r / min; (4) The fourth stage of fermentation (81h- 168h), the fermentation pH was controlled to be 5.0, the temperature was 26°C, the stirring speed was 80r / min, and the fermentation was completed until 168h.
[0056]The extraction and determination method of Ganoderma lucidum triterpenoids is the same as in Example 1, and the yield of Ganod...
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