Preparation method and preparation device of polylysine

A polylysine and preparation device technology, applied in the field of polylysine preparation method and its preparation device, can solve problems such as human side effects, and achieve the effect of easy control and regulation

Inactive Publication Date: 2020-08-14
樟树市狮王生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used antiseptic measures are to add food preservatives. Food preservatives are divided into chemical preservatives, natural preservatives and compound preservatives. Among them, chemical preservatives are the most widely used. Long-term, stable and

Method used

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  • Preparation method and preparation device of polylysine
  • Preparation method and preparation device of polylysine
  • Preparation method and preparation device of polylysine

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Experimental program
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Embodiment 1

[0024] Embodiment 1 of the present invention relates to a preparation method of polylysine, which includes the following steps: seed selection, expanded cultivation, fermentation preparation, and filtration and clarification;

[0025] The specific implementation method of the seed selection step is: select the mixed strain of Streptomyces albicans and ergot fungus as the polylysine strain.

[0026] The specific implementation method of the expanded cultivation step is: under aseptic conditions, transplant the selected polylysine strain into a prepared petri dish for cultivation, and the petri dish uses glucose as a carbon source and peptone as a nitrogen source , formulated with inorganic salts and growth factors; the polylysine strains are carried out on the petri dish for 3 to 5 times, and the maintenance time of each expansion culture is 4 to 6 days; the temperature of each expansion culture is controlled at 23 to 29 ° C, The cultivation humidity was kept at 60-65%.

[002...

Embodiment 2

[0030] see Figure 1~2 , in Embodiment 2 of the present invention, a preparation device related to the preparation method of polylysine in Embodiment 1 is provided, which includes: a preparation device body 9, a sealing cover 10 is installed on the upper end of the preparation device body 9, and the preparation The left side of the device body 9 is provided with an oxygen supply pipe 6 and a liquid level gauge 8, and the oxygen supply pipe 6 is connected through a two-way pipe 7, and the preparation device body 9 is connected to the oxygen tank 5 through the oxygen supply pipe 6; the preparation device body 9 The right side is connected to the booster pump 15 through the booster pipe 14; the left and right sides of the lower end of the preparation device body 9 are provided with a discharge pipe 16, and the discharge pipe 16 is provided with a discharge valve, and the bottom end of the preparation device body 9 is installed and fixed on the working on stage 4;

[0031] A ther...

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Abstract

The invention belongs to the field of polylysine products, and particularly relates to a preparation method and a preparation device of polylysine. The preparation method of polylysine comprises the steps of seed selection, enlarged culture, fermentation preparation and filtration clarification. A polylysine product produced by the preparation method of polylysine is stable in passage and high inpolylysine production capacity; in addition, related experiments find that the polylysine product produced by the preparation method of polylysine is high in antibacterial ability, good in fresh-keeping effect and relatively high in safety; more importantly, compared with a traditional biological fermentation device, the preparation device for producing the polylysine product can easily control and adjust all related physical parameters in the fermentation condition, and a stirring assembly is arranged according to the electromagnetic induction driving principle, so that the problems that a rotating shaft is mounted on the sealing cover, the sealing cover is inconvenient to open, and the preparation device is easy to leak in the fermentation process due to the fact that the bottom end of the preparation device penetrates through the rotating shaft can be avoided.

Description

technical field [0001] The invention relates to the field of polylysine products, in particular to a preparation method of polylysine and a preparation device thereof. Background technique [0002] Food spoilage will cause huge economic losses, how to prevent spoilage is a problem that food scientists are most concerned about. At present, the commonly used antiseptic measures are to add food preservatives. Food preservatives are divided into chemical preservatives, natural preservatives and compound preservatives. Among them, chemical preservatives are the most widely used. Long-term, the effect of use is stable and continuous, but it also has certain side effects on the human body. With the gradual enhancement of people's health awareness, the application of natural preservatives and compound preservatives will become more and more extensive, and the market potential is huge. [0003] ε-Polylysine is a natural biological metabolite with good bactericidal ability and therma...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P13/02C12N1/14C12N1/20C12M1/38C12M1/36C12M1/34C12M1/12C12M1/04C12M1/02C12M1/00C12R1/47C12R1/645
CPCC12M23/22C12M23/38C12M23/48C12M27/02C12M29/06C12M41/12C12M41/40C12M41/44C12M47/10C12N1/14C12N1/20C12P13/02C12P39/00
Inventor 洪立芝
Owner 樟树市狮王生物科技有限公司
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