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Method of catalytically synthesizing sucrose-6-acetate by Rhizomucor miehei lipase

A technology of Rhizomucor miehei and lipase, which is applied in the direction of fermentation, etc., can solve the problems of poor selectivity, harsh reaction conditions, multi-color by-products, etc., achieve low cost, simple production process, and avoid low-efficiency transesterification reaction Effect

Pending Publication Date: 2020-08-25
ANHUI JINGHE IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of Rhizomucor miehei lipase in order to solve the problems of harsh reaction conditions, poor selectivity, many by-products and deep product color in the chemical synthesis process of existing sucrose-6-acetate Catalyzed method for synthesizing sucrose-6-acetate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a. Put 1.2m³ of DMF and 4.8m³ of tert-amyl alcohol into the reaction kettle (6m³), mix well, and get the organic solution mixture;

[0022] b. Put 123kg (0.06 moL / L) of sucrose into the reactor, start stirring to dissolve completely, then add 310kg of vinyl acetate (0.6 moL / L) and 480kg of Rhizomucor miehei lipase into the reactor, stirring (200 rpm ), insulated at 30°C for 9 hours, after the reaction was completed, the Rhizomucor miehei lipase in the reaction system was removed by filtering through a filter press, and the filtrate was placed at room temperature for 30 minutes;

[0023] c. Perform vacuum distillation on the filtrate, control the temperature at 60°C and the pressure at - 0.085 Mpa, collect the liquid in the condensate tank and cool it (4.5m³) to room temperature, then add 4m³ of dichloromethane, after turbidity, add dichloromethane again Methane 3m³, stand still, flocculent crystals precipitate to the bottom of the kettle, and filter to obtain the mot...

Embodiment 2

[0027] a. Put 2.5m³ of DMF and 10.0m³ of tert-amyl alcohol into the reaction kettle (15m³), mix well, and get the organic solution mixture;

[0028] b. Put 250kg (0.06 moL / L) of sucrose into the reactor, start stirring to dissolve completely, add 630kg of vinyl acetate (0.6 moL / L), 1000kg of Rhizomucor miehei lipase into the reactor, and stir (250 rpm ), insulated at 30°C for 9 hours, after the reaction was completed, the Rhizomucor miehei lipase in the reaction system was removed by filtering through a filter press, and the filtrate was placed at room temperature for 25 minutes;

[0029] c. Perform vacuum distillation on the filtrate, control the temperature at 60°C and the pressure at -0.090Mpa, collect the liquid in the condensate tank and cool it (9.3m³) to room temperature, then add 9m³ of dichloromethane, after turbidity, add dichloromethane again Methane 6m³, stand still, flocculent crystals precipitate to the bottom of the kettle, and filter to obtain the mother liq...

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Abstract

The invention relates to a method of catalytically synthesizing sucrose-6-acetate by Rhizomucor miehei lipase. The method is characterized by comprising the steps of a. adding DMF and tert-pentyl alcohol into a reaction kettle according to a volume ratio of 1:(3 to 5), and performing uniform mixing; b, adding sucrose into a reaction kettle; adding vinyl acetate and the Rhizomucor miehei lipase, controlling the concentration of the sucrose to be 0.03 to 0.12 mmoL / mL, the concentration of the Rhizomucor miehei lipase to be 6 to 60 mg / mL, the mol ratio of the sucrose to the vinyl acetate to be 1:(5 to 20) and the temperature to be 30 to 35 DEG C to react for 8 to 24 h, performing filtration, and placing filter liquid at a room temperature for 20 to 40 min; c, performing reduced pressure distillation on the filter liquid at the temperature of 50 to 70 DEG C and the pressure of -0.080 to -0.1 MPa, cooling liquid in a condensing groove to the room temperature, adding dichloromethane, addingthe dichloromethane again after turbidity occurs, controlling the ratio of the total quantity of the dichloromethane to the volume of the condensing liquid to be (4 to 7):3, performing still standingand filtration to obtain mother liquid; and d, pumping the mother liquid into a centrifugal machine to obtain faint yellow crystals. The method provided by the invention has the advantages that the reaction conditions are mild; the operation is simple; the generation of isomers is avoided; the catalytic selectivity, the product safety and the conversion rate are high; and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of chemical production, relates to the field of sucralose production, in particular to a method for synthesizing sucrose-6-acetate catalyzed by Rhizomucor miehei lipase. Background technique [0002] Sucralose is a fresh sweetener. Sucralose is a new type of sweetener jointly developed by the British Taylor Company and the University of London and applied for a patent in 1976. It is the only functional sweetener with sucrose as raw material. odorant. Sucralose has a diluting effect on sour and salty tastes, has a masking effect on unpleasant tastes such as astringency, bitterness, and alcohol, and has a synergistic effect on spicy and milky tastes. It has a wide range of applications. Therefore, this product is the most ideal intense sweetener today and can be consumed by children, teenagers, youth, middle-aged, elderly and patients with various diseases without any nutritional doubts. The methods for synt...

Claims

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Application Information

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IPC IPC(8): C12P19/12
CPCC12P19/12
Inventor 张正颂祁飞肖士东郑学连
Owner ANHUI JINGHE IND
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