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Method for fermenting chymosin by using nisin fermentation wastewater as partial nitrogen source

A technology of nisin and fermentation waste liquid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of nisin pollution of the environment and the high cost of rennet, reduce pollution, cost reduction effect

Active Publication Date: 2012-07-25
AMTECH BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem that the cost of producing chymosin by microbial fermentation is high, and the fermentation waste liquid of nisin is directly discharged to pollute the environment. enzymatic approach

Method used

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specific Embodiment approach 1

[0009] Specific embodiment one: the present embodiment utilizes nisin fermented waste liquid to make the method for partial nitrogen source fermentation to produce chymosin, carry out according to the following steps: 1, the preparation of culture medium: NaH 2 PO 4 、Na 2 HPO 4 , glucose, high-temperature amylase, Tween 80, corn flour, corn starch, nisin fermentation waste liquid and soybean tissue protein were dissolved in water to prepare a medium, in which NaH 2 PO 4 : 3.25g / L, Na 2 HPO 4 : 3.11g / L, glucose: 2.14g / L, high temperature amylase: 20g / L, Tween 80: 0.13g / L, corn flour: 30~80g / L, corn starch: 0~30g / L, lactic acid chain Mycocin fermentation waste liquid: 150-700g / L, soybean textured protein: 0-20g / L; use 5%-6% sodium hydroxide to adjust the pH to 6.7-6.8, and sterilize at 0.14MPa pressure for 30min 2. The preparation of Rhizomucor miehei seed liquid: inoculate the inoculum of Rhizomucor miehei into the seed culture medium with an inoculum of 4% by volume, and...

specific Embodiment approach 2

[0012] Embodiment 2: This embodiment differs from Embodiment 1 in that: the concentration of corn flour added in Step 1 is 35-70 g / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0013] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the concentration of corn flour added in Step 1 is 50-60 g / L. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a method for fermenting chymosin by using nisin fermentation wastewater as a partial nitrogen source, relating to the method for producing the chymosin by microbial fermentation. The invention solves the problems that at current, the method for producing the chymosin by the microbial fermentation has high cost, and the direct discharge of the fermentation wastewater of the nisin results in environment pollution. The method comprises the following steps: 1, preparing a culture medium; 2, preparing rhizomucor miehei seed liquor; 3, inoculating the rhizomucor miehei seed liquor to a triangular flask; and 4, culturing the inoculated seed liquor for 96h at the temperature of 37-38 DEG C to obtain the chymosin. The invention can utilize waste, reduce the environment pollution caused by directly discharging the fermentation wastewater of the nisin, and lower the cost for fermenting the chymosin. The invention can be applied to the field of microbial fermentation.

Description

technical field [0001] The invention relates to a method for producing chymosin by microbial fermentation. Background technique [0002] Rennet belongs to acid protease, also known as aspartic acid protease, which is an indispensable preparation for cheese production. The traditional source of rennet is the abomasum of lactating calves. Animal rennet and its high ratio of milk coagulation activity to proteolytic ability have become the enzyme of choice for making cheese, but the animal grows slowly and is expensive, and it is easy to increase the enterprise The cost, and the procedure and process of extracting enzyme liquid from animals are more complicated. Plants such as papaya, fig, pineapple, difficulty, acacia, ginkgo, etc. all contain proteases that can coagulate milk, but plant-derived chymosin has too high proteolytic activity or is itself toxic, so many plant-based rennets No large-scale commercial application has been obtained. [0003] Microbial chymosin has a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/58C12R1/645
Inventor 郭坤王贵元马之霄马莉张钦革黄亦存
Owner AMTECH BIOTECH
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