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Method for improving secernment efficiency of recombined protein

A recombinant protein and efficiency technology, applied in the biological field to achieve the effect of improving the efficiency of recombinant protein secretion

Inactive Publication Date: 2010-11-10
WUXI KINGENEW BIOTECHNOLOGY LIMITED COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because the recombinant protein does not enter the lumen of the endoplasmic reticulum containing complex protein complexes during non-canonical secretion, the decrease in secretion efficiency due to transport block caused by steric conformation does not occur

Method used

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  • Method for improving secernment efficiency of recombined protein
  • Method for improving secernment efficiency of recombined protein
  • Method for improving secernment efficiency of recombined protein

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Abstract

The invention provides a method for improving recombinant protein secretion efficiency, which is characterized in that a secretion signal sequence SS in the homeotic allotype domain of the mouse transcription factor Engrailed 2 is fused to the N-terminal of the recombinant protein; wherein the amino acid sequence of the secretion signal sequence SS is that: Ala Gln Glu Leu Ser Leu Asn Glu Ser Gln. The invention has the advantages that: the secretion efficiency of human catalase using the invention is improved to 2.3 times than the normal secretion efficiency guided by bovine Beta-casein signal peptide and provides an effective solution for improving recombinant protein secretion efficiency in industrial production.

Description

A method for improving the secretion efficiency of recombinant protein technical field The invention relates to the field of biotechnology, in particular to a method for improving the secretion efficiency of recombinant proteins. Background technique Humans have used recombinant DNA technology to produce therapeutic pharmaceutical proteins for decades. At present, people mainly use prokaryotic bacterial expression system and eukaryotic yeast and mammalian cell expression system to produce recombinant medicinal protein. Bacteria grow fast, and it is relatively easy to genetically modify them, especially in industrial production, where the cost is much lower than the large-scale culture system of mammalian cells. However, due to some inherent defects of the bacterial expression system, it is difficult to express some complex proteins derived from higher eukaryotes. One of the main reasons is that bacteria lack a perfect post-translational modification system, which easily ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/62C12N15/09A61P17/18A61K38/16A61K38/44C12N15/53
Inventor 何祖勇汤波李宁
Owner WUXI KINGENEW BIOTECHNOLOGY LIMITED COMPANY
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