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Genetically engineered bacterium capable of efficiently expressing alpha-transglucosidase, and construction method of genetically engineered bacterium

A technology of glucose transsidase and genetically engineered bacteria, applied in the field of genetic engineering, can solve problems such as patent publications that have not yet been found, and achieve the effects of reducing the cost of extraction and purification, huge economic and social benefits, and improving secretion efficiency

Inactive Publication Date: 2013-12-11
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Genetically engineered bacterium capable of efficiently expressing alpha-transglucosidase, and construction method of genetically engineered bacterium
  • Genetically engineered bacterium capable of efficiently expressing alpha-transglucosidase, and construction method of genetically engineered bacterium
  • Genetically engineered bacterium capable of efficiently expressing alpha-transglucosidase, and construction method of genetically engineered bacterium

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Embodiment Construction

[0033] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0034] The main idea of ​​the present invention is: the α-glucosidase gene is cloned and placed in other host strains for heterologous expression, because of the relationship of glycosylation, the properties of the transglycosidase will change, affecting the conversion rate of IMO, and at the same time There is also the problem of codon preference in source expression. Only by introducing it into its own host strain and performing homologous expression in the original strain can its yield be increased without changing its properties. Aspergillus niger, as an α-glucosidase production strain, is not only an excellent expression system, but also a food-grade safe production bacterium with strong protein secretion ability. Therefore, the cons...

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Abstract

The invention relates to a genetically engineered strain efficiently expressing alpha-transglucosidase. A host bacterium of the genetically engineered strain is aspergillus niger, and the genetically engineered strain comprises an alpha-transglucosidase gene expression cassette. A genetically engineered bacterium can efficiently express alpha-transglucosidase, and improves the secretion efficiency of alpha-transglucosidase. In addition, alpha-transglucosidase is secreted to the outside of a cell, so that the cost of alpha-transglucosidase extraction purification is lowered, industrial materials are saved, the production cost is lowered, and the genetically engineered strain has an obvious social benefit. The enzyme activity of a crude enzyme obtained after the strain is fermented is improved by 4.0-12.1 times in comparison to a starting strain; a foundation is laid for an application of alpha-transglucosidase in the food industry and industrial production; a bottleneck that alpha-transglucosidase depends on import is broken; and the genetically engineered strain has huge economic and social benefits, and has a broad market development prospect.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a genetic engineering strain and a construction method thereof, in particular to a genetic engineering strain highly expressing α-glucosidase and a construction method thereof. Background technique [0002] α-glucosidase (α-transglucosidase, EC 2.4.1.24), also known as α-glucosidase, transfer glucosidase, it can cut α-1 from the non-reducing end of the oligosaccharide substrate, 4 glycosidic bonds, release glucose, or transfer free glucose residues to another sugar substrate to form α-1, 6 glycosidic bonds, so as to obtain non-fermentable functional isomaltooligosaccharides (IMO), glycolipids or glycopeptides etc. α-glucosidase is an important industrial enzyme, which has shown broad application prospects in food, feed, chemical industry, medicine and other fields, especially the huge application potential in IMO production has already aroused the attention of the food...

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Application Information

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IPC IPC(8): C12N1/15C12N15/54C12N15/63C12R1/685
Inventor 黎明路福平刘萌
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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