Efficient, broad-spectrum keratinase and its gene

A technology of keratinase and gene, applied in genetic engineering, plant genetic improvement, hydrolytic enzymes, etc., can solve problems such as high-efficiency strains that cannot be considered as genetic engineering

Inactive Publication Date: 2003-03-26
姚斌 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although domestic research on keratinase was carried out earlier, they all focused on the isolation of strains. At present, a gene encoding keratinase has not been isolated, let alone the construction of highly efficient strains of genetic engineering.

Method used

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  • Efficient, broad-spectrum keratinase and its gene
  • Efficient, broad-spectrum keratinase and its gene
  • Efficient, broad-spectrum keratinase and its gene

Examples

Experimental program
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Embodiment Construction

[0016] experiment one

[0017] This experiment illustrates the screening procedure for natural strains producing keratinase.

[0018] Feather keratin was used as the only N source, and keratinase-producing bacteria were screened by continuous enrichment culture. Rotten feather soil samples were diluted routinely and inoculated into the screening medium (1.5g / LKH 2 PO 4 , 0.025g / L MgSO 4 .7H 2 O, 0.025g / L CaCl, 0.015g / L FeSO 4 .7H 2 O, 0.005g / L ZnSO 4 , 0.5g / L feather meal, pH 6.0), cultured at 30°C for 10-15 days, picked the bacilli that propagated and grown in this culture medium and cultured them continuously for 3 rounds, then coated and separated on the screening medium plate, picked Take a single colony and re-inoculate it into the screening culture medium, and then repeat plate separation and liquid screening culture for the well-grown strains to purify the strains. 24 strains secreting keratinase were screened by this method.

[0019] In...

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Abstract

The invention provides a keratinase and its coding gene. It has advantages of high activity, good heat stability, wide PH range and efficient degradation of alpha & beta-keratinase. It has also the character of common protease and can degrade many kinds of protein derived from plants and animals. It can be widely used in the trades such as feed, foods, washing, wool spinning and pharmacy.

Description

technical field [0001] The present invention relates to a kind of keratinase and its gene. technical background [0002] Keratinase is a class of enzymes that can hydrolyze keratin, and some keratinases can also hydrolyze various proteins except keratin. Keratin is characterized by highly interconnected cysteine ​​disulfide bonds, insoluble in water, salt, dilute acid and dilute alkali, and cannot be degraded by general proteases. Keratin is the main component of animal feathers, hair, and hooves, and is widely found in human and animal skin, scars, and scabs. [0003] Keratinase can be used in the feed industry to convert waste such as feathers and pig hair into high-nutrition feed protein. Keratin is the waste produced during poultry processing—the main component of feathers. The protein content in feather meal is as high as 90%, and the content of essential amino acids is extremely high. It is an excellent protein source in animal feed, but it contains cysteine Formed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N15/57
Inventor 姚斌王亚茹操时树史秀云袁铁峥范云六
Owner 姚斌
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