Biological manufacturing method for utilizing waste feathers to produce amino acid liquid fertilizer

A technology of amino acids and feathers, which is applied in liquid fertilizers, fertilizer mixtures, nitrogen fertilizers, etc., can solve the problems of chlorine-sensitive plant toxicity, large environmental pollution, and high sodium ion concentration, reduce energy consumption and pollution, and have simple and stable processes. Raw materials Effects from a wide range of sources

Active Publication Date: 2019-04-19
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And all there is certain shortcoming in these several methods, as utilizing acid hydrolysis, environmental pollution is bigger, and chlorine salt content is higher in the amino acid stoste, produces poisonous effect to some chlorine-sensitive plants; And utilize enzymatic hydrolysis, because Due to the raw materials and process of enzymolysis, the content of

Method used

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  • Biological manufacturing method for utilizing waste feathers to produce amino acid liquid fertilizer
  • Biological manufacturing method for utilizing waste feathers to produce amino acid liquid fertilizer
  • Biological manufacturing method for utilizing waste feathers to produce amino acid liquid fertilizer

Examples

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Embodiment 1

[0032] 1.1 Materials and reagents

[0033] Feathers: Feathers are provided by Guangdong Bowater Biotechnology Co., Ltd., which belong to chicken feathers. They are thoroughly washed and dried for later use.

[0034] Keratinase (100,000 U / g), self-produced; neutral protease (100,000 U / g), alkaline protease (100,000 U / g), lipase (200,000 U / g), papain (100,000 U / g), Yangshao Biochemical Technology Co., Ltd.; amino acid peptidase (100,000 U / g, exopeptidase) Angel Yeast Co., Ltd.; acid protease (50,000 U / g), Yuanye Biotechnology Co., Ltd.; NaOH , HCl, sodium citrate, sulfosalicylic acid, trichloroacetic acid, potassium sulfate, EDTA-Na and other reagents are analytically pure; mixed amino acid standard solution, chromatographically pure.

[0035] 1.2 Instruments and equipment

[0036] Coordinating laboratory instruments, automatic amino acid analyzers, Kjeldahl nitrogen analyzers, centrifuges, etc.

[0037] 1.3 Method

[0038] 1.3.1 Process flow

[0039] Collecting feathers—cl...

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Abstract

The invention discloses a method for utilizing waste feathers to produce amino acid liquid fertilizer. The method comprises the steps of carrying out enzymolysis on feather powder through compound enzyme to obtain enzymolysis liquid, adding acid protease into the enzymolysis liquid for enzymolysis, filtering the liquid after enzymolysis is finished, and carrying out enzyme inactivation on a filtrate to obtain the amino acid liquid fertilizer. The compound enzyme comprises keratinase and amino acid peptidase. According to the method, the enzymolysis rate of the feathers can reach 80% or above,the prepared amino acid liquid fertilizer contains 17 kinds of amino acids, the variety is complete, the content of the amino acids can reach 10.12% or above (mass fraction), the content of small peptide can reach 9.39% (mass fraction), and the method can meet the national standard for the amino acid liquid fertilizer without concentration. Therefore, the environment-friendly amino acid liquid fertilizer can be prepared.

Description

technical field [0001] The invention belongs to the technical field of fertilizer processing, and specifically discloses a method for preparing amino acid liquid fertilizer by using keratinase, neutral protease, alkaline protease, amino acid peptidase and acid protease to enzymatically hydrolyze waste feathers step by step. Background technique [0002] Feathers are the remaining waste after poultry processing. Worldwide, millions of tons of discarded feathers are produced every year, and the utilization rate of resources is less than 20%. Most of these feathers are treated as waste except a small amount is used as thermal clothing filler and animal feed, which not only causes waste of resources, but also causes serious pollution to the environment through traditional incineration, landfill and other treatment methods. Therefore, the management of feather waste has become an urgent problem for poultry slaughtering enterprises. However, feather waste is a rich natural protei...

Claims

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Application Information

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IPC IPC(8): C05C11/00
CPCC05C11/00C05G5/20Y02A40/20C05F1/005C05F11/10C05G5/23
Inventor 朱红惠谢小林周莲陈美标姚青
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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