Microbial feed additive and preparation method thereof

A technology for the preparation of microbial feed and additives, applied in the field of agricultural biology, can solve the problems of refractory degradation and low utilization rate of digestion, and achieve the effects of promoting digestion, absorption and utilization, improving intestinal flora and improving economic benefits.

Active Publication Date: 2010-10-27
山东省农业科学院高新技术研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Feather has become an important feed protein raw material resource in my country. The annual discarded feather resources in China have exceeded 1 million tons (the annual feed protein raw material gap in my country exceeds 10 million tons), and feather meal is mainly ad

Method used

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  • Microbial feed additive and preparation method thereof
  • Microbial feed additive and preparation method thereof
  • Microbial feed additive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation method of microbial feed additive, the steps are as follows:

[0030] (1) Preparation of seed fermentation broth

[0031] Strain selection: select Bacillus subtilis (Bacillus subtilis) YYW-1, CGMCC NO.2396; under aseptic conditions, inoculate the strain in the inclined test tube into the liquid feather fermentation seed medium, and the medium capacity of 500mL Erlenmeyer flask is 100~ 150mL, shake culture at 37°C for 18 hours to obtain shake flask seed solution;

[0032]With the inoculum amount of 3% volume ratio, inoculate the shake flask seed solution in a 2-ton fermenter equipped with 1.3 tons of feather fermentation medium, cultivate at a temperature of 37°C, and carry out aeration and stirring culture, wherein the stirring speed is 220r / in , ventilation rate 1:1.1, tank internal pressure 0.1±0.02MPa, ferment for 13 hours to obtain seed tank fermentation liquid; culture, stirring speed is 250r / min, ventilation rate is 1:1.1, tank inter...

Embodiment 2

[0039] Embodiment 2: the preparation method of microbial feed additive: the preparation of seed fermentation liquid is the same as embodiment 1. The difference is:

[0040] (2) Fermentation tank culture:

[0041] With the inoculum amount of 10% volume ratio, inoculate the fermented liquid of the seed tank in a 20-ton fermenter equipped with 13 tons of feather fermentation medium, cultivate at a temperature of 38°C, and cultivate with aeration and agitation, wherein the agitation speed is 220r / min, and the aeration The amount is 1:1.1, the pressure in the tank is 0.1±0.02MPa, the culture is stopped when the spore production rate of the bacteria reaches 90%, and the fermentation period is 33 hours;

[0042] The above-mentioned feather fermentation medium and its preparation method are as follows: in parts by weight, 1 part of feather meal, 1 part of mannitol, 1 part of glucose, 1 part of sodium citrate, 0.2 part of sodium chloride, 0.01 part of magnesium sulfate, and 0.01 part ...

Embodiment 3

[0045] Embodiment 3: the preparation of microbial feed additive, the preparation of seed fermentation liquid is the same as embodiment 1. The difference is:

[0046] (2) Fermentation tank culture:

[0047] With the inoculum amount of 10% volume ratio, inoculate the fermented liquid of the seed tank in a 20-ton fermenter equipped with 13 tons of feather fermentation medium, cultivate at a temperature of 38°C, and cultivate with aeration and agitation, wherein the agitation speed is 220r / min, and the aeration The amount is 1:1.1, the pressure in the tank is 0.1±0.02MPa, the culture is stopped when the spore production rate of the bacteria reaches 90%, and the fermentation period is 44 hours;

[0048] The culture medium for fermentation tank and its preparation method are: in parts by weight, 5 parts of feather meal, 2 parts of mannitol, 2 parts of glucose, 3 parts of sodium citrate, 0.3 part of sodium chloride, 0.04 part of magnesium sulfate, and 0.04 part of calcium chloride ...

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Abstract

The invention relates to a method for preparing a microbial feed additive. Feather meal, mannitol, glucose, sodium citrate, sodium chloride and the like are taken as raw materials, and inoculated with Bacullus subtilis YYW-1 with the number of CGMCC NO.2396 to prepare the microbial feed additive by submerged fermentation and spin flash drying technology. The active ingredients of the microbial feed additive are the Bacullus subtilis and cultures thereof, and the cultures contain metabolites such as keratinase capable of efficiently degrading feather. The product can be used in the breeding industry and the feed industry, and has the advantages of improving intestinal flora, improving digestion and absorption capacity of animals and the feed conversion and utilization rate, particularly promoting the digestion, absorption and utilization of protein materials such as the feather meal, and reducing cost.

Description

technical field [0001] The invention relates to a microbial feed additive and a preparation method thereof, belonging to the field of agricultural biotechnology. Background technique [0002] Microbial feed additive is a new type of safe and green feed additive, which can promote growth, prevent and resist disease, improve product quality, and benefit the environment. modern animal husbandry. [0003] my country is currently a major producer and exporter of livestock products, but in recent years, the export of livestock products, especially meat, has been repeatedly restricted, mainly due to antibiotic and drug residues, and imperfect disease prevention and control systems. The use of antibiotics as growth promoters and disease prevention and treatment in livestock and poultry breeding has a history of more than half a century. In the past 30 years, long-term use of antibiotics has caused endogenous infection or superinfection of animals, and the emergence of drug-resistan...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 岳寿松游银伟尤升波王翠萍邹晓凤朱友峰
Owner 山东省农业科学院高新技术研究中心
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