Bacterial cellulose foam fermentation method

A bacterial cellulose and foam technology, applied in the field of microbial fermentation, can solve the problems of low fermentation level, unaffordable price, and low output, and achieve the effects of optimal structural strength, improved utilization rate, and excellent performance

Active Publication Date: 2016-12-07
SHAN DONG NAMEIDE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical obstacles for bacterial cellulose to be industrialized mainly include: 1. Low fermentation level, low output, high cost, and price not worth ordinary plant cellulose; Technology has not yet been resolved

Method used

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  • Bacterial cellulose foam fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A better bacterial cellulose foam fermentation method, comprising:

[0062] 1) Preparation of strains. Take the strains stored at -80°C, transfer them to -20°C for 12 hours, transfer them to 4°C and place them for about 2-4 hours until they are completely dissolved.

[0063] 2) Inoculate 1ml of bacterial solution into 150ml of seed culture solution and incubate at 28°C for 24 hours to become a first-class seed solution.

[0064] 3) Take the first-grade seed liquid and inoculate it into 500ml of fermented liquid, the purpose is to carry out bacterium cell amplification, and the formula of fermented liquid and seed culture liquid is different. Cultivate at 30°C for 18-24 hours.

[0065] 4) Add 1% foaming agent A and 2% foam stabilizer B to the fermentation broth, and mix thoroughly in a 500ml airtight blue cap bottle.

[0066] 5) Pour the fermentation liquid into the foaming cylinder. The foaming cylinder is a high-temperature sterilizable PE barrel with an air inlet a...

Embodiment 2

[0073] A better bacterial cellulose foam fermentation method, comprising:

[0074] 1) Preparation of strains. Take the strains stored at -80°C, transfer them to -20°C for 12 hours, transfer them to 4°C and place them for about 2-4 hours until they are completely dissolved.

[0075] 2) Inoculate 1ml of bacterial solution into 150ml of seed culture solution and incubate at 28°C for 24 hours to become a first-class seed solution.

[0076] 3) Take the first-grade seed liquid and inoculate it into 500ml of fermented liquid, the purpose is to carry out bacterium cell amplification, and the formula of fermented liquid and seed culture liquid is different. Cultivate at 30°C for 18-24 hours.

[0077] 4) Add 5% foaming agent A and 10% foam stabilizer B to the fermentation broth, and mix thoroughly in a 500ml airtight bottle.

[0078] 5) Pour the fermentation liquid into the foaming cylinder. The foaming cylinder is a high-temperature sterilizable PE barrel with an air inlet at the bo...

Embodiment 3

[0081] A better bacterial cellulose foam fermentation method, comprising:

[0082] 1) Preparation of strains. Take the strains stored at -80°C, transfer them to -20°C for 12 hours, transfer them to 4°C and place them for about 2-4 hours until they are completely dissolved.

[0083] 2) Inoculate 1ml of bacterial solution into 150ml of seed culture solution and incubate at 28°C for 24 hours to become a first-class seed solution.

[0084] 3) Take the first-grade seed liquid and inoculate it into 500ml of fermented liquid, the purpose is to carry out bacterium cell amplification, and the formula of fermented liquid and seed culture liquid is different. Cultivate at 30°C for 18-24 hours.

[0085] 4) Add 3% foaming agent A and 6% foam stabilizer B to the fermentation broth, and mix thoroughly in a 500ml airtight bottle.

[0086] 5) Pour the fermentation liquid into the foaming cylinder. The foaming cylinder is a high-temperature sterilizable PE barrel with an air inlet at the bot...

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Abstract

The invention provides a bacterial cellulose foam fermentation method. The method comprises the steps of inoculating fermentation liquor with fermentation strain for foaming; collecting foam, and conducting standing fermentation to obtain bacterial cellulose foam. The fermentation liquor contains a foaming agent and a foam stabilizer. Uniform and stable foam is formed by means of a small amount of culture medium, all the culture medium is located on the surface of gas liquid, nutrients of the culture medium are fully utilized, the culture medium utilization rate is increased, and time for reaching the fermentation endpoint is shortened. The steps are simple, operation is convenient, and practicality is high.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a bacterial cellulose foam fermentation method. Background technique [0002] Bacterial cellulose is another kind of natural and non-toxic nanomaterials synthesized by microbial fermentation besides plant cellulose, also called microbial cellulose. The chemical structure of bacterial cellulose is the same as that of ordinary cellulose, but it has superior characteristics that ordinary cellulose cannot match. Bacterial cellulose belongs to nano-scale fibers and is currently the thinnest among natural fibers. A typical bacterial fiber bundle is only 0.1 μm wide, while coniferous wood pulp fibers are at least 30 μm wide, and even cotton fibers are about 15 μm wide , and bacterial cellulose exists in the form of 100% cellulose, with extremely high purity, and has characteristics such as good permeability, high tensile strength and excellent property maintenance ability. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12M1/36C12M1/04C12M1/02
CPCC12M27/02C12M29/06C12M41/00C12P19/04
Inventor 戚明刘景君孔蕊刘凯
Owner SHAN DONG NAMEIDE BIOTECHNOLOGY CO LTD
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