Preparation method of keratin

A technology of keratin and compound protease, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of insoluble, resource waste, and environmental damage, and achieve good stability, environmental protection, and environmental protection. pollution effect

Active Publication Date: 2014-08-13
北京中农探味科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0002] The hoof horns of animals are rich in protein (about 80% to 85%) and minerals such as calcium and phosphorus, but the keratin in it is derived from ectoderm differentiation and belongs to a fibrous hard protein. Due to its unique properties , is a potential protein resource, but keratin contains more disulfide bonds and is not easy to dissolve, so it has not been fully developed and utilized. The output of horn is more than one million tons, and it is usually thrown away as waste, which not only causes a waste of resources, but also damages the environment

Method used

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Examples

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Embodiment 1

[0032] like figure 1 Shown, the method for preparing keratin, comprises the steps:

[0033] 1) Put the cleaned croissant raw material into a reaction tank, add drinking water with a weight 3 times that of the croissant, adjust the pH to 13.0 with a NaOH solution with a mass volume concentration of 5%, and boil it at 100° C. for 2 hours. Exclude the lye, wash it with clean water for 3 times, remove the bone plug and dry it at 65°C for 3 hours, then crush it. 350-400rpm, and then use a universal grinder to further grind the croissant particles. The speed of the universal grinder is 3000-3500rpm, and then pass through a 40-mesh sieve (sieve size: 0.425mm) to collect the undersize, that is, the particle size is less than or Equivalent to 0.425mm of croissant.

[0034] 2) Put the croissant powder obtained in step 1) into NaOH aqueous solution of pH 13.0 whose weight is 6 times its weight, raise the temperature to 100°C and stir for alkaline hydrolysis, the required stirring speed...

Embodiment 2

[0044] A preparation method of keratin, comprising the steps of:

[0045] 1) Preprocessing:

[0046] First, put the raw material trotter carapace containing keratin into the third aqueous solution whose weight is 4 times its weight, use the NaOH solution with a mass volume concentration of 7.5% to adjust the pH of the third aqueous solution to 13.5, and treat it at a temperature of 100° C. for 2 hours, Then get rid of the third aqueous solution, remove the bone plug, wash 3 times with clean water, and then dry the pig's hoof nail at a temperature of 70°C for 4 hours, and finally break the dried raw material containing keratin, and pass through 80 mesh (sieve hole size : 0.180mm) nylon mesh sieve to collect the undersize, that is, particles with a particle size less than or equal to 0.180mm.

[0047] 2) Alkaline hydrolysis: first put the trotter granules with a particle size less than or equal to 0.180 mm obtained in step 1) into the first aqueous solution of pH 113.5 for alka...

Embodiment 3

[0053] A preparation method of keratin, comprising the steps of:

[0054] 1) Preprocessing:

[0055] Firstly, put the raw trotter hoof carapace containing keratin into the third aqueous solution whose weight is 2.5 times, adjust the pH of the third aqueous solution to 13.1 by using NaOH solution with a mass volume concentration of 6%, and treat it at a temperature of 93°C for 1.8h , then exclude the third aqueous solution, remove the bone plug, wash it twice with clean water, then dry the pig’s hoof nail at a temperature of 65°C for 3 hours, and finally break the dried raw material containing keratin, and use a 60-mesh (sieve hole) Dimensions: 0.250mm) Nylon mesh filter to collect the undersize, that is, particles with a particle size less than or equal to 0.250mm.

[0056] 2) Alkaline hydrolysis: first put trotter hoof granules with a particle size less than or equal to 0.250 mm into the first aqueous solution of pH 13.2 for alkaline hydrolysis for 2.2 hours, wherein the NaO...

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Abstract

The invention discloses a preparation method of a keratin. The preparation method comprises the following steps: I, adding a raw material comprising keratin to a first aqueous solution for alkaline hydrolysis, and carrying out solid-liquid separation to obtain a first keratin solid; II, adding the first keratin solid into a second aqueous solution, adding a proper amount of compound protease for enzymolysis for 4-5 hours under the conditions that the temperature is 48-52 DEG C and the pH is 8.0-9.0; then, inactivating the compound protease; then, adding a proper amount of keratinase for enzymolysis for 2.5-3.5 hours at 35-40 DEG C; and finally, carrying out solid-liquid separation to obtain a second keratin solid; III, first, shading and oxidizing the second keratin solid for 3.5-4.5 hours by using an oxidizing agent, and then, carrying out solid-liquid separation to obtain a third keratin solid; and IV, drying the third keratin solid till the water content is below 5-8%, wherein the dried third keratin solid is the target keratin.

Description

technical field [0001] The invention relates to a preparation method of keratin. Background technique [0002] The hoof horns of animals are rich in protein (about 80% to 85%) and minerals such as calcium and phosphorus, but the keratin in it is derived from ectoderm differentiation and belongs to a fibrous hard protein. Due to its unique properties , is a potential protein resource, but keratin contains more disulfide bonds and is not easy to dissolve, so it has not been fully developed and utilized. The output of horn is more than one million tons, and it is usually thrown away as waste, which not only causes waste of resources, but also damages the environment. Contents of the invention [0003] One of the purposes of the present invention is to provide a new method for preparing keratin; [0004] Another object of the present invention is to provide a keratin. [0005] The technical scheme provided by the present invention is: [0006] A preparation method of kerat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/12
Inventor 张春晖王春青王金枝
Owner 北京中农探味科技有限公司
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