Enzymolysis feather powder being high in digestion coefficient and preparing method of enzymolysis feather powder

The technology of high digestibility enzyme and digestibility enzyme is applied in the field of high-efficiency enzymatic hydrolysis of feather meal and its preparation, which can solve the problems of large amount of water added, unsatisfactory product smell, difficult drying, etc. low water effect

Inactive Publication Date: 2018-09-07
广东希普生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has milder reaction conditions and higher biological potency, but the amount of water added

Method used

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  • Enzymolysis feather powder being high in digestion coefficient and preparing method of enzymolysis feather powder

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Effect test

Embodiment 1

[0026] The preparation method of the high digestibility enzymolysis feather meal of the present embodiment comprises the following steps:

[0027] 1) Preparation of Bacillus licheniformis seed solution: Weigh 5.0g of peptone, 3.0g of beef extract, 5.0g of NaCl, add water to make up to 1000mL, adjust the pH to 7.0, sterilize at 121°C for 30min, cool to 30°C ℃, after inoculating the Bacillus licheniformis strain, culture it on a shaker at 120 rpm and 37° C. for 18 hours to obtain the Bacillus licheniformis seed liquid.

[0028] 2) Preparation of secondary strains of Bacillus licheniformis: Weigh 7kg of soybean meal powder, 3kg of corn flour, add 4kg of water and mix, then sterilize the mixture of soybean meal, corn flour and water at 121°C for 1 hour, and cool to 37°C , inoculate 1000 mL of the Bacillus licheniformis seed liquid in step 1), spread it to a thickness of 5 cm, and incubate at 37° C. for 24 hours to obtain the secondary strain of Bacillus licheniformis.

[0029] 3)...

Embodiment 2

[0037] The preparation method of the high digestibility enzymolysis feather meal of the present embodiment comprises the following steps:

[0038] 1) Preparation of plant lactic acid bacteria seed liquid: weigh 10.0g of casein peptone, 10.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 2.0g of diammonium citrate, 1.0g of Tween 80, 2.0 g K 2 HPO 4 , 0.2g MgSO 4 .7H 2 O, 0.05g MnSO 4 .H 2 O, 20.0 g CaCO 3 , add water to the volume to 1000mL, adjust the pH to 6.8, sterilize at 121°C for 20 minutes, cool to 30°C, inoculate Lactobacillus plantarum strains, and cultivate on a shaker at 130rpm and 37°C for 18h to obtain plant Lactobacillus seed liquid.

[0039] 2) Preparation of secondary strains of plant lactic acid bacteria: Weigh 6kg of soybean meal powder, 4kg of corn flour, add 4kg of water and mix well, then sterilize the mixture of soybean meal powder, corn flour and water at 121°C for 0.5h, and cool to 30°C , inoculate 1000 mL of the ...

Embodiment 3

[0046] The preparation method of the high digestibility enzymolysis feather powder of the present embodiment of the present embodiment comprises the following steps:

[0047] 1) Preparation of Bacillus licheniformis seed liquid: Weigh 5.0g of peptone, 3.0g of beef extract, and 5.0g of NaCl, add water to make the volume 1000mL, adjust the pH to 7.0, sterilize at 121°C for 20min, and cool to 25 ℃, after inoculating the Bacillus licheniformis strain, culture it on a shaker at 160 rpm and 37° C. for 20 hours to obtain the Bacillus licheniformis seed solution.

[0048] 2) Preparation of secondary strains of Bacillus licheniformis: Weigh 7kg of soybean meal powder, 3kg of corn flour, add 4kg of water and mix well, then sterilize the mixture of soybean meal, corn flour and water at 125°C for 0.5h, and cool to 40 ℃, inoculate 1000 mL of the Bacillus licheniformis seed solution in step 1), spread it to a thickness of 10 cm, and culture it at 40° C. for 18 hours to obtain the secondary ...

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Abstract

The invention relates to enzymolysis feather powder being high in digestion coefficient and a preparing method of the enzymolysis feather powder. The preparing method comprises the following steps offirstly, mixing feather with water in the weight ratio of the feather to the water being (1 to 0.4) to (1 to 3), then performing cooking for 0.3-3h, wherein the cooking temperature is 100-200 DEG C, and the cooking pressure is 80-500kPa; adding keratinase of which the enzyme activity unit is 100-500 thousand to the cooked feather in the weight ratio of the keratinase to the feather being 0.3-2%, and performing enzymolysis under the enzymolysis condition that the enzymolysis temperature is 50-70 DEG C and the enzymolysis pH is 7-9 for 12-36h; and cooling the feather after being subjected to enzymolysis to 20-40 DEG C, inoculating the cooled feather with one or two or three kinds of strains of bacillus licheniformis, saccharomycetes or lactobacillus plantarum in the weight ratio of the strains to the feather being 0.1-0.5%, adjusting the water content by mass to 50-70%, and performing fermenting under the condition that the fermentation temperature is 20-40 DEG C, and the fermentation pHis 5-7 for 24-72h, so as to obtain the enzymolysis feather powder. An animal feed prepared from the enzymolysis feather powder high in digestion coefficient is high in the digestibility by animals, and good in palatability.

Description

technical field [0001] The invention belongs to the technical field of protein feed, and in particular relates to a high-efficiency enzymolysis feather meal and a preparation method thereof. Background technique [0002] my country is a country that lacks protein resources. The protein raw materials for conventional feeds, including soybean meal and fish meal, mainly rely on imports and are expensive. There is an urgent need to find new protein raw materials with high quality and low price. The crude protein content in feathers is more than 80%, the amino acid content is more than 70%, and contains the essential amino acids needed by many animals. However, feather protein is a kind of keratin composed of highly cross-linked disulfide bonds, which is not easily digested by the stomach. Protease, trypsin and other general proteases are degraded, so it is difficult to be directly digested and utilized by animals, and special processing is required. [0003] At present, the com...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/14A23K10/26A23K10/30A23K10/37A23K20/147
CPCA23K10/12A23K10/14A23K10/26A23K10/30A23K10/37A23K20/147Y02P60/87
Inventor 程林春恽辉余忠丽劳泰财王俊青伍爱辉
Owner 广东希普生物科技股份有限公司
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