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Keratinase-proudicng bacterium and its preparation method

A technology for keratinase and bacteria production, applied in the field of keratinase production bacteria and its preparation

Inactive Publication Date: 2006-07-12
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, the production of keratinase by bacterial fermentation is still blank

Method used

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  • Keratinase-proudicng bacterium and its preparation method
  • Keratinase-proudicng bacterium and its preparation method
  • Keratinase-proudicng bacterium and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0050] Isolation and screening of strains:

[0051] 1) Sampling from slaughterhouses in Yunnan Yiliang, Jianshui, Yuxi, etc., feather keratin-rich garbage soil, sewer silt, sewer water, and soil from poultry activity sites, using feather keratin as the sole nitrogen source for continuous enrichment Set training. Take 5g of soil sample into 50mL enrichment culture solution and add 3-5 sterilized intact feathers. The enrichment medium consists of (g / L): NH 4 Cl0.5, NaCl0.5, K 2 HPO 4 0.3, KH 2 PO 4 0.4, MgCl 2 ·6H 2 O 0.1, yeast extract 0.1, feather meal 10, enriched culture at 37℃, 200r / min for 3-10 days, after feathers are decomposed, transfer the culture solution to 50mL enriched culture solution, and proceed under the same conditions The second enrichment culture.

[0052] 2) Choose the above-mentioned culture medium with better degradation of feathers, and follow the conventional dilution gradient (10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 ) After dilution, carry out plate coating ...

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Abstract

The invention relates to a keratinase generating bacteria and it's preparing method. The systematic name is Bacillus subtilis with the registration number: CGMCC No.1505. It sieves the best strains of the keratinase whose best PH value of the course enzyme liquid enzyme reaction is about 7.5-8.0 and the best temperature is 50-60 deg. It keeps the temperature in 70 deg. water-bath with 30 min, the surplus enzyme is about 90%; it keeps the temperature with 120 min, the surplus enzyme is about 70%.

Description

Technical field [0001] The invention relates to a keratinase-producing bacteria and a preparation method thereof. Background technique [0002] Keratin exists in nature in large quantities in the main form of various animal hairs, feathers, hoof horns, etc., and is a hard protein with strong resistance. The keratin molecule forms a highly cross-linked three-dimensional structure through the disulfide bond, hydrogen bond, salt bond and other cross bonds of cystine. It is very stable and insoluble in water, salt, dilute acid and dilute alkali, and it is not easy for most Degraded by proteolytic enzymes (such as trypsin, casein, pepsin, etc.). Keratinase is a class of enzymes that can specifically degrade natural keratin, which can significantly improve the digestibility of keratin. Some keratinases also hydrolyze a variety of proteins except keratin. [0003] Keratinase can specifically decompose the characteristics of keratin, so that it has broad application prospects in the feed...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
Inventor 黄遵锡钟巧芳许波
Owner YUNNAN NORMAL UNIV
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