High-nucleic-acid baker's yeast and preparation method thereof

A baker's yeast and baker's yeast technology, applied in biochemical equipment and methods, microorganism-based methods, fungi, etc., can solve the problem of affecting the content of high nucleic acid yeast, yeast nucleic acid yield not reaching the expected effect, and progeny yeast nucleic acid. Content differences and other issues, to achieve the effect of improving purity, increasing nucleic acid content, and promoting expression

Pending Publication Date: 2019-08-30
乐斯福(明光)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the difference in the nucleic acid content of the primary yeast, there is a large difference in the nucleic acid content of the progeny yeast obtained from the proliferation of th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1

[0016] A high-nucleic acid baker's yeast, relative to 100% by weight of baker's yeast cell, contains more than 21% by weight of nucleic acid, and the weight of RNA is more than 13%.

[0017] A preparation method of high nucleic acid baker's yeast, specifically according to the following steps:

[0018] S1: Activate the yeast cells, place the frozen yeast strains in a sterile room, and dissolve them naturally at room temperature, then transfer the yeast liquid to the liquid culture medium, and conduct activation culture in a constant temperature incubator at 34~38℃ for 18 hours ;

[0019] S2: Screen the dominant bacteria, use a low-speed centrifuge, divide the yeast liquid into a 50ml test tube, centrifuge at a low speed of 200r / min for 5 minutes, collect the bacteria gathered at the bottom of the test tube, and place the bacteria in the liquid medium Shake gently, continue culturing for 18 hours and then repeat the low-speed centrifugation to obtain the dominant ye...

Example Embodiment

[0024] Example 2

[0025] A high-nucleic acid baker's yeast, relative to 100% by weight of baker's yeast cell, contains more than 21% by weight of nucleic acid, and the weight of RNA is more than 13%.

[0026] A preparation method of high nucleic acid baker's yeast, specifically according to the following steps:

[0027] S1: Activate the yeast cells, place the frozen yeast strains in a sterile room, and dissolve them naturally at room temperature, then transfer the yeast liquid to the liquid culture medium, and conduct activation culture in a constant temperature incubator at 36°C for 24 hours;

[0028] S2: Screen the dominant bacteria, use a low-speed centrifuge, divide the yeast liquid into a 50ml test tube, centrifuge at a low speed of 250r / min for 5 minutes, collect the bacteria gathered at the bottom of the test tube, and place the bacteria in the liquid medium Shake gently, continue culturing for 24 hours and then repeat low-speed centrifugation to obtain the dominant yeast cell...

Example Embodiment

[0033] Example 3

[0034] A high-nucleic acid baker's yeast, relative to 100% by weight of baker's yeast cell, contains more than 21% by weight of nucleic acid, and the weight of RNA is more than 13%.

[0035] A preparation method of high nucleic acid baker's yeast, specifically according to the following steps:

[0036] S1: Activate the yeast cells, place the frozen yeast strains in a sterile room, and dissolve them naturally at room temperature, then transfer the yeast liquid to the liquid culture medium, and conduct activation culture in a constant temperature incubator at 38°C for 28 hours;

[0037] S2: Screen the dominant bacteria, use a low-speed centrifuge, divide the yeast liquid into a 50ml test tube, centrifuge at a low speed of 300r / min for 5 minutes, collect the bacteria gathered at the bottom of the test tube, and place the bacteria in the liquid medium Shake gently, continue culturing for 28 hours and then repeat the low-speed centrifugation to obtain the dominant yeast ...

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Abstract

The invention discloses high-nucleic-acid baker's yeast and a preparation method thereof. Compared with 100% weight of baker's yeast thalli, the high-nucleic-acid baker's yeast contains 21wt% or aboveof nucleic acid, wherein the weight of RNA is greater than 13%. Yeast is subjected to activation and culture, and the fermentation, growth and proliferation activity of the yeast are promoted throughliquid mediums having high-concentration glucose, so that the expression of RNA substances in microzyme cells is promoted, and increase of the whole nucleic acid content of primary-generation microzyme cells is facilitated; and then through low-speed centrifugal separation, microzyme containing more nucleic acid components and larger in unicellular weight is separated, so that the purity of the high-nucleic-acid yeast is further improved, filial-generation yeast cells obtained through subsequent enlarged culture contain high nucleic acid content, and the nucleic acid content of the produced microzyme is increased wholly.

Description

technical field [0001] The invention belongs to the technical field of baker's yeast, and in particular relates to a high-nucleic acid baker's yeast and a preparation method thereof. Background technique [0002] Yeast nucleic acid is ribonucleic acid extracted from natural yeast. At present, it is mainly used in medicine, health food and infant food. With the development of modern biological extraction technology, yeast nucleic acid is widely used, especially in new anticancer drugs and health products. and baby food. When using yeast to produce nucleic acid, the existing practice usually directly uses the primary yeast for expanded culture, and then processes the yeast cells to obtain yeast nucleic acid. Due to the difference in the nucleic acid content of the primary yeast, the nucleic acid content of the progeny yeast obtained from the proliferation of the primary yeast is quite different, which affects the content of high-nucleic acid yeast to a certain extent, and ult...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N1/06C12R1/865
CPCC12N1/18C12N1/06
Inventor 朱永兵
Owner 乐斯福(明光)有限公司
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