Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sesame drought-resistant gene SiSAM1 and application thereof

A sesame and genetic technology, applied in the sesame drought-resistant gene SiSAM1 and its application field, can solve the problems of weak drought resistance of sesame and few molecular related studies, and achieve the effect of improving drought resistance, improving drought resistance, and improving drought resistance

Inactive Publication Date: 2019-02-05
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
View PDF3 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the drought resistance of sesame is relatively weak. The research reports mainly focus on the impact of drought on the morphology, yield traits, quality traits and physiological and biochemical characteristics of sesame plants, and there are few molecular related studies.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sesame drought-resistant gene SiSAM1 and application thereof
  • Sesame drought-resistant gene SiSAM1 and application thereof
  • Sesame drought-resistant gene SiSAM1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Acquisition of Sesame Drought Resistance Gene SiSAM1 Gene

[0029] 1. Discovery of the drought resistance gene SiSAM1 in sesame

[0030] 1. From the 7910 domestic and foreign resources preserved in the National Sesame Medium-term Bank, according to the phenotype, geographical origin and genetic diversity detection results of drought resistance-related traits, a step-by-step sampling strategy was adopted, and 400 sesame materials with different drought resistance were selected for re-analysis. Sequencing analysis;

[0031] 2. Using the Illumina Hiseq2500 sequencing platform, the 2×76 double-end sequencing method was used to resequence the whole genome of 400 sesame materials with low coverage, and obtained a genome sequence with 2.6 times coverage;

[0032] 3. Combining the data of drought-resistance-related traits, genotype data and population structure in the drought-resistant population of germplasm resources, the EMMAX software package and Peal program wer...

Embodiment 2

[0038] Example 2 Construction and genetic transformation of SiSAM1 gene overexpression vector

[0039] 1. Construction of overexpression vector

[0040]The drought-resistance related sesame gene SiSAM1 that embodiment 1 is cloned utilizes the method for homologous recombination and pCAMBIA1301S (provided by this laboratory) plasmid connection construction plant expression vector, named after pCAMBIA1301S-SiSAM1 ( figure 1 ), the specific operations are as follows:

[0041] 1. First, the linearized vector was obtained by double enzyme digestion (BamHI and KpnI) (Takara), and then purified by agarose gel electrophoresis and gel recovery kit (Tiangen Biochemical Technology Co., Ltd.) to obtain a high-purity linearized vector.

[0042] 2. Add the DNA of the target fragment and the linearized carrier to a 1.5ml centrifuge tube at a molar ratio of 3:1 for recombination reaction, mix well and place it at 37°C for about 30min, add 10μl of the reaction solution to 50μl of DH5a compete...

Embodiment 3

[0074] Implementation 3 Determination of Drought Resistance of Positive Plants of SiSAM1 Overexpression Transgenic T2 Generation Families

[0075] Three transgenic T2 generation family plants overexpressing the SiSAM1 gene in Example 2 were selected to carry out the drought stress experiment. The specific steps are as follows: plant 3 plants of each material in the nutrient pot (when the T2 generation transgenic plants grow to the stage of 3 pairs of leaves) and 3 plants of non-transgenic wild-type Arabidopsis thaliana plants, when the plants start to boot, the water is cut off to carry out drought stress for 17 days, and then observe the growth status of the transgenic plants.

[0076] The results show that the growth of the 3 transgenic plants overexpressed by the SiSAM1 gene cloned by the present invention is slightly better than that of the control plant under normal conditions but the difference is not very large, but after the adversity stress treatment, the growth of th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of gene engineering, and provides a sesame drought-resistant gene SiSAM1 and application thereof. The sesame drought-resistant gene SiSAM1 is a nucleotidesequence as shown by SEQ ID NO.1; or a nucleotide sequence with drought-resistant performance generated by adding, substituting, inserting or omitting on or more nucleotide sequences of the nucleotidesequence as shown by SEQ ID NO.1. The SiSAM1 gene is established onto an expression vector, the SiSAM1 gene is transferred into Arabidopsis by virtue of agrobacterium-mediated conversion method to obtain transgenic agrobacterium; the transgenic agrobacterium and non-transgenic agrobacterium are simultaneously treated in a drought stress manner, and comparing the transgenic agrobacterium with thenon-transgenic agrobacterium, the expression and drought-resistant capacity of SiSAM1 can be greatly improved. By adopting the sesame drought-resistant gene SiSAM1, the drought-resistant capacity of plants can be improved, and the sesame drought-resistant gene SiSAM1 can be used for the breeding of an oil crop drought-resistant variety, so that the drought-resistant performance of crops can be improved, and the high yield and stable yield of the crops can be ensured.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the sesame drought-resistant gene SiSAM1 and its application. Background technique [0002] Sesamum (Sesamum indicum L.) is an important oil crop, mainly distributed in tropical and subtropical regions from 40°N to 40°S. Due to the high nutritional value of sesame seeds (oil content 40%-62.7%; protein content 19%-30%), sesame has the reputation of "queen of oil". Sesame is one of the five major oil crops in my country and is a traditional characteristic agricultural product. As a major producer and trading country, my country's annual consumption ranks first in the world, but its self-sufficiency is less than 50%, and its import rate is increasing year by year. [0003] Due to the combined effects of biotic and abiotic stresses, the annual yield of sesame is low and unstable, which is also a major bottleneck in the development of the sesame industry. Sesame is main...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/20
CPCC07K14/415C12N15/8273
Inventor 黎冬华山姆张秀荣周瑢玛丽刘爱丽游均魏鑫张艳欣王林海
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products