Mutant gene Osnaat1-1 of rice rich in iron and zinc

A rice and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of lack of rice germplasm resources, iron content and distribution can not meet the needs of nutritional breeding, etc., to improve the absorption and accumulation capacity, express enhanced effect

Inactive Publication Date: 2007-12-26
ZHEJIANG UNIV
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Problems solved by technology

In recent years, the International Rice Institute has analyzed the iron content of nearly a thousand rice germplasm resources, and found that the iron content and distribution of the so-called high-iron rice varieties obtained through traditional breeding methods cannot meet the needs of nutritional breeding (Welsh &Graham, 1999; Dr. Barry of the International Rice Institute, 2006, Harvestplus-China annual meeting speech), that is to say, there is no rice germplasm resource available for rice iron-rich breeding

Method used

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  • Mutant gene Osnaat1-1 of rice rich in iron and zinc

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, the screening of mutant:

[0047] Seeds in the rice mutant (Nipponbare) bank were soaked at 37°C to accelerate germination and sowed into the culture solution (standard formula of the International Rice Institute, Yoshida et al. 1976). The nutrient solution contained 40ppm N(NH4NO3), 10ppm P(NaH2PO4·H2O), 40ppm K(K2SO'), 40ppm Ca(CaCl2@2H2o), 40ppm Mg(MgSO·7H2O) and trace elements such as Mn, Mo, B, Zn, Cu and Fe. The final concentration of Fe in the solution is 36 μM, supplied as citrate-Fe 3+ . Float a gauze on the nutrient solution, put the rice seeds on the gauze, grow for 10 days under the conditions of 80% relative humidity, 30° C. during the day and 22° C. at night, and perform mutant screening. A mutant with severe growth retardation, yellowing of above-ground part, shortened adventitious root and lateral root, and lateral root growth extending down to the part near the root tip was screened. The mutants withered and died after growing in this m...

Embodiment 2

[0048] Example 2, Responses of mutant naat1 to different iron forms:

[0049] Mutants cannot grow on Citrate-Fe containing 3+ or FeCl 3 etc Fe 3+ In the environment where the form exists, but when we culture him in the environment containing EDTA-Fe 2+ or FeSO 4 etc Fe 2+ It can grow normally in the form of nutrient solution (Figure 1). And in Fe 2+ The seedlings cultivated in the nutrient solution can grow and develop normally and bear fruit (Fig. 2) like the wild type under the condition of flooding soil culture.

Embodiment 3

[0050] Embodiment 3, the gene cloning of mutant:

[0051] Mutant naat1 crossed with indica rice Kasalath to obtain F1 generation hybrids, F 1 Planting Harvested F 2 Substitute seeds, direct seeding to rice basic nutrient solution (Citrate-Fe 3+ ), cultured for 10 days to observe the segregation, the results showed that the segregation ratio of the wild phenotype and the mutant phenotype conformed to the segregation rule of 3:1, which proved that the mutation was a recessive mutation controlled by a single gene. In F2, 30 individuals with mutant phenotypes were selected to extract DNA, and the SSR analysis method was used for preliminary positioning. According to the SSR polymorphic markers provided by the Gramene (http: / / www.gramene.org / ) website, each chromosome Select reasonably distributed SSR markers (about 5), first in a small amount of F 2 Coarse mapping was performed within the population (about 30), and the mutant gene was located on the short arm of the second chro...

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Abstract

This invention discloses a protein coded by rice Osnaat1-1 gene, which has the amino acid sequence as shown in SEQ ID No.2. The rice Osnaat1-1 gene has the nucleotide sequence as shown in SEQ ID No.1. This invention also discloses a method for modifying rice. The method comprises: modifying rice by conventional gene mutation technique with Osnaat1-1 gene sequence to obtain rice Osnaat1-1 mutant as shown in SEQ ID No.1. The mutant is rich in Fe and Zn.

Description

technical field [0001] The invention belongs to the fields of crop breeding and genetic engineering, and relates to a new rice gene and its function. Specifically, it relates to rice Osnaat1-1 (gene Osnaat1 mutation formation) gene and application thereof. Background technique [0002] The trace element iron plays a very important role in plant nutrition and human health. Today, the number of people in the world with insufficient iron intake due to iron deficiency in crops has reached one billion. However, the ability of plants to utilize iron in soil is very low because most iron is fixed in the soil in an insoluble form and cannot be used by plants (Curie and Briat, 2003). Therefore, increasing the iron content in plants, especially crops, has huge benefits for socio-economic and human health. In recent years, the International Rice Institute has analyzed the iron content of nearly a thousand rice germplasm resources, and found that the iron content and distribution of ...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82
Inventor 吴平寿惠霞程龙军王芳郑录庆
Owner ZHEJIANG UNIV
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