Biological fermentation production technology of hyaluronic acid

A bio-fermentation and production process technology, applied in the field of hyaluronic acid bio-fermentation production process, can solve the problems that restrict the production of hyaluronic acid, and achieve the effects of promoting accumulation, increasing production and promoting expression

Inactive Publication Date: 2018-01-16
成都远泓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production of hyaluronic acid is severely restricted due to the imbalance between the bacteria's own metabolism and product expression.

Method used

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  • Biological fermentation production technology of hyaluronic acid
  • Biological fermentation production technology of hyaluronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The present embodiment provides a biofermentation production process of hyaluronic acid, which includes the following steps:

[0035] Step 1: The strain is activated, and the strain is inoculated on the slant medium. The formula of slant medium is: glucose 20g / L, sucrose 2g / L, yeast powder 5g / L, beef extract 3g / L, peptone 3g / L, K 2 HPO 4 1.6g / L, MgSO 4 ·7H 2 O 0.4g / L, agar 2% (mass volume fraction). In an incubator, culture at 37°C for 12 hours to obtain activated strains.

[0036] Step 2: Preparation of seed liquid, inoculate the activated bacteria into a 1L Erlenmeyer flask with sterilized seed medium, and cultivate it for 16 hours at 37°C and 170r / min to obtain seed liquid, and observe the seed liquid under microscope The growth status of the bacteria. The formula of seed medium is: glucose 25g / L, sucrose 5g / L, yeast powder 8g / L, beef extract 4g / L, peptone 4g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O 0.4g / L.

[0037] Step 3: Expand the culture. If the bacteria i...

Embodiment 2

[0040] This example provides a bio-fermentation production process of hyaluronic acid, which is the same as steps 1 to 3 of the bio-fermentation production process of hyaluronic acid provided in Example 1, the difference is that step 4 in this example is performed as follows :

[0041] Step 4: Fermentation culture. If the growth of the bacteria in the expanded culture medium is good and there is no contamination by bacteria, inoculate the expanded culture medium according to the ratio of expanded culture medium:fermentation medium = 1:6 (v:v) until the 240L 600L fermentation tank for fermentation medium, the formula of fermentation medium is: glucose 37g / L, sucrose 8g / L, yeast powder 11g / L, urea 6g / L, peptone 6g / L, K 2 HPO 4 2.4g / L, MgSO 4 ·7H 2 O 0.5g / L. Keep the ventilation at 4L / min, 200r / min, pH 7.3, and culture at 37°C for 4h. Then keep the ventilation rate at 8L / min, 300r / min, pH 7.3, and the temperature at 32°C, culture for 16h, and add fresh fermentation culture ...

Embodiment 3

[0043] This example provides a bio-fermentation production process of hyaluronic acid, which is the same as steps 1 to 3 of the bio-fermentation production process of hyaluronic acid provided in Example 1, the difference is that step 4 in this example is performed as follows :

[0044] Step 4: Fermentation culture. If the growth of the bacteria in the expanded culture medium is good and there is no contamination by bacteria, inoculate the expanded culture medium according to the ratio of expanded culture medium: fermentation medium = 1:7 (v:v) until the 245L 600L fermentation tank for fermentation medium, the formula of fermentation medium is: glucose 37g / L, sucrose 8g / L, yeast powder 12g / L, urea 8g / L, peptone 8 / L, K 2 HPO 4 2.2g / L, MgSO 4 ·7H 2 O 0.8g / L. Keep the ventilation at 4L / min, 220r / min, pH 7.3, and culture at 37°C for 4h. Then keep the ventilation rate at 4L / min, 250r / min, pH 7.3, and the temperature at 32°C, cultivate for 20h, and add fresh fermentation culture...

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Abstract

The invention discloses a biological fermentation production technology of hyaluronic acid and belongs to the field of biological fermentation. The biological fermentation production technology of thehyaluronic acid, disclosed by the invention, comprises the following steps: activating strains, culturing a seed solution, carrying out amplified culture on the strains and carrying out fermentationculture. The fermentation culture is divided into a fermentation earlier stage and a fermentation later stage; the fermentation earlier stage and the fermentation later stage are applicable to self-growth of thalli and accumulation of the hyaluronic acid respectively. According to the production technology disclosed by the invention, a self-growth process of the thalli and a product expression process are separated relatively to realize the balance of self-metabolism of the thalli and product expression, so that the expression of the hyaluronic acid is promoted; nutrient components in a fermentation process are improved so that the growth of the thalli and the accumulation of the hyaluronic acid can be promoted better; a class index material supplementing manner is used for fermenting according to a growth principle of the thalli, so that the yield of the hyaluronic acid is improved.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a biological fermentation production process of hyaluronic acid. Background technique [0002] Hyaluronic acid (HA), also known as hyaluronic acid, has a high degree of water retention, emollient and antibacterial protection, and is widely used as an additive in cosmetics. As a biochemical drug, HA is mainly used in the treatment of arthritis, ophthalmic viscosity surgery, soft tissue repair, and viscosity inhibitor after surgery. HA is an acidic mucopolysaccharide composed of D-glucuronic acid and acetylamino-D-glucose in a 1:1 molar ratio. In the inner wall of the plasma membrane, under the action of HA synthase, HA is synthesized from the two precursors of uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) and uridine diphosphate-glucuronic acid (UDP-GlcA). HA has no species difference, regardless of the source, its structure and composition are the same, only the di...

Claims

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Application Information

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IPC IPC(8): C12P19/26
Inventor 黄明良王铎学吴晖晖杨廷敏徐大勇蒲小平
Owner 成都远泓生物科技有限公司
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