Biological fermentation production technology of hyaluronic acid
A bio-fermentation and production process technology, applied in the field of hyaluronic acid bio-fermentation production process, can solve the problems that restrict the production of hyaluronic acid, and achieve the effects of promoting accumulation, increasing production and promoting expression
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[0033] Example 1
[0034] The present embodiment provides a biological fermentation production process of hyaluronic acid, which comprises the following steps:
[0035] Step 1: Activation of strains, inoculation of strains on the slant medium. The formula of the slant medium is: glucose 20g / L, sucrose 2g / L, yeast powder 5g / L, beef extract 3g / L, peptone 3g / L, K 2 HPO 4 1.6g / L, MgSO 4 ·7H 2 O 0.4g / L, agar 2% (mass volume fraction). In an incubator, cultured at 37 °C for 12 h to obtain activated strains.
[0036] Step 2: Seed liquid preparation, inoculate activated strains in a 1L conical flask containing sterilized seed medium, cultivate at 37°C and 170r / min for 16 hours to obtain seed liquid, and observe the seed liquid by microscopic examination. growth status of the bacteria. The seed medium formula is: glucose 25g / L, sucrose 5g / L, yeast powder 8g / L, beef extract 4g / L, peptone 4g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O 0.4g / L.
[0037] Step 3: Expand the culture. If t...
Example Embodiment
[0039] Example 2
[0040] This embodiment provides a biological fermentation production process of hyaluronic acid, which is the same as steps 1 to 3 of the biological fermentation production process of hyaluronic acid provided in Example 1. The difference is that in this embodiment, step 4 is performed as follows :
[0041] Step 4: Fermentation culture, if the growth condition of the cells in the expanded culture medium is good and there is no contamination by miscellaneous bacteria, the expanded culture medium is inoculated to 240L according to the ratio of expanded culture medium: fermentation medium=1:6 (v:v) Fermentation medium 600L fermentation tank, fermentation medium formula: glucose 37g / L, sucrose 8g / L, yeast powder 11g / L, urea 6g / L, peptone 6g / L, K 2 HPO 4 2.4g / L, MgSO 4 ·7H 2 O 0.5g / L. Keep the ventilation rate at 4L / min, 200r / min, pH 7.3, and culture at 37°C for 4h. Then keep the ventilation rate at 8L / min, 300r / min, pH 7.3, temperature at 32°C, culture for...
Example Embodiment
[0042] Example 3
[0043] This embodiment provides a biological fermentation production process of hyaluronic acid, which is the same as steps 1 to 3 of the biological fermentation production process of hyaluronic acid provided in Example 1. The difference is that in this embodiment, step 4 is performed as follows :
[0044] Step 4: Fermentation culture, if the growth condition of the cells in the expanded culture medium is good and there is no contamination of miscellaneous bacteria, the expanded culture medium is inoculated according to the ratio of expanded culture medium: fermentation medium = 1:7 (v:v) to contain 245L. Fermentation medium 600L fermentation tank, fermentation medium formula: glucose 37g / L, sucrose 8g / L, yeast powder 12g / L, urea 8g / L, peptone 8 / L, K 2 HPO 4 2.2g / L, MgSO 4 ·7H 2 O 0.8g / L. Keep the ventilation rate at 4L / min, 220r / min, pH 7.3, and culture at 37°C for 4h. Then keep the ventilation rate at 4L / min, 250r / min, pH 7.3, temperature at 32°C, cu...
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