SUMO and SUMO protease encoding gene and application thereof

A protease and gene technology, applied in the fields of genetic engineering and protein engineering, to achieve high expression, increase expression, and broaden application
CN106434699AInactive Publication Date: 2017-02-22ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a SUMO (small ubiquitin-related modifier) and SUMO protease encoding gene, encoding protein and application thereof. The invention relates to a Saccharomyces cerevisiae gene sumo (S), which is defined by the nucleotide sequence of SEQ ID No.1 in the sequence table. The encoding protein of the Saccharomyces cerevisiae gene sumo (S) is defined by the amino acid sequence of SEQ ID No.2 in the sequence table. The SUMO protease gene ulp (S) provided by the invention is defined by the nucleotide sequence of SEQ ID No.3 in the sequence table. The encoding protein of SUMO protease gene ulp (S) provided by the invention is defined by the amino acid sequence of SEQ ID No.4 in the sequence table. The invention can realize high expression of Saccharomyces cerevisiae sumo gene and SUMO protease ulp gene in Escherichia coli, specifically realize high expression in the commonly used expression strain BL21(DE3) of Escherichia coli, and ensures that the function is not affected.
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Description

technical field

[0001] The invention relates to the fields of genetic engineering and protein engineering, in particular to SUMO and SUMO protease genes, encoded proteins and applications thereof. Background technique

[0002] The Escherichia coli expression system is often used to express recombinant proteins, but there are also some defects. When some foreign proteins are overexpressed, insoluble and non-functional inclusion body precipitation will often be formed [Villaverde A, Carrio M, M.2003. Proteinaggregation in recombinant bacteria : biological role of inclusionbodies.Biotechnol letters.25(17):1385-1395], fusion tag expression has been shown to be used to improve the yield and folding of foreign recombinant proteins [Esposito D, Chatterjee D, K.2006.Enhancement of soluble protein expression through the use of fusion tags. Curr. Opin. in Biotechnol. 17(4):353-358]. Traditional fusion tags include maltose-binding protein MBP, glutathione S-transferase GST, thioredoxi...

Claims

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