Making method of aggrecanases inhibitor combined with in-vitro cross-linked hyaluronic acid hydrogel and application thereof in drugs for treating osteoarthritis

Abstract

The invention discloses a making method of an aggrecanases inhibitor combined with in-vitro cross-linked hyaluronic acid hydrogel and application thereof in drugs for treating osteoarthritis. The making method comprises the steps of aldehyde group modification on hyaluronic acid, adipic dihydrazide modification on hyaluronic acid and in-vitro cross-linking of hyaluronic acid. The aggrecanases inhibitor combined with in-vitro cross-linked hyaluronic acid hydrogel provided by the invention has properties of sustained releasing drugs, can effectively alleviate rat osteoarthritis, promote expression of proteoglycan of osteoarthritis knees and inhibit expression of degradation products of proteoglycan. According to the making method of the aggrecanases inhibitor combined with the in-vitro cross-linked hyaluronic acid hydrogel and application thereof in drugs for treating osteoarthritis, biological materials and drugs are combined, a tissue engineering method is adopted for treating osteoarthritis, the manner of delivering drugs to articular cavities is utilized, wound caused by treatment of osteoarthritis is reduced, the sustained release drug delivery manner can improve local drug concentration while reducing corresponding side effect caused by systemic administration of drugs.

Description

technical field

[0001] The invention relates to the application of a proteoglycanase inhibitor combined with externally linked hyaluronic acid hydrogel, in particular to its application in the preparation of medicines for treating osteoarthritis. Background technique

[0002] Osteoarthritis is one of the most common orthopedic diseases. According to statistics, in the West alone, about 12% of the middle-aged and elderly people suffer from different degrees of osteoarthritis. In the United States, there are more than 27 million people suffering from osteoarthritis, and the prevalence rate of people over 60 years old can reach more than 50%. A study by the Center for Disease Control and Prevention of the United States and the Department of Public Health of Harvard University shows that osteoarthritis is One of the five major diseases that cause disability in older adults. Osteoarthritis is characterized by the collapse and necrosis of articular surface cartilage, accompanied ...

Images