Application of cucumber CsWRKY50 gene to strengthening downy mildew resistance of cucumber

A cucumber downy mildew and cucumber technology, applied in the field of crop genetic engineering, can solve the problems of pesticide pollution, yield decline, etc., and achieve the effects of safe application methods, improved resistance, and enhanced resistance
CN108486149AActive Publication Date: 2018-09-04SHANDONG AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG AGRICULTURAL UNIVERSITY
Publication Date
2018-09-04

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Abstract

The invention discloses application of a cucumber CsWRKY50 gene to strengthening the downy mildew resistance of the cucumber, the specific application is to overexpress the cucumber CsWRKY50 gene in acucumber plant, and the nucleotide sequence of the cucumber CsWRKY50 gene is shown as in SEQ ID:1. That overexpressing the CsWRKY50 in the cucumber can obviously improve the resistance of the cucumber to the downy mildew is found for the first time, the experiment proves that the cucumber CsWRKY50 gene is transferred into the cucumber plant as a target gene, the expression of the resistance geneis increased by changing the expression quantity of the cucumber CsWRKY50 gene in the cucumber plant, the resistance of the cucumber to the downy mildew is improved, the character is used in the breeding of the cucumber, the cucumber variety capable of resisting the downy mildew is bred by choosing the cucumber CsWRKY50 gene overexpressed plant through the gene detection method, the application method is safe and reliable, and the application has an excellent application prospect in the researches of the resistant variety of the cucumber.
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Description

technical field

[0001] The invention relates to the technical field of crop genetic engineering, in particular to the application of a cucumber CsWRKY50 gene in enhancing cucumber downy mildew resistance. Background technique

[0002] Cucumber (Cucumis sativus L.) is a herbaceous plant of Cucurbitaceae, which is a vegetable crop widely cultivated in the world. Cucumber has many health effects and is deeply loved by people. At present, our country is mainly cultivated in greenhouses and greenhouses. Due to the special climatic conditions and continuous cropping in greenhouses and greenhouses, cucumbers are vulnerable to various diseases, among which downy mildew, powdery mildew, and gray mold are the most serious. The onset and spread of these diseases are fast, causing a series of problems such as a substantial decline in yield, poor quality, and premature plant aging. In order to solve this problem, people usually rely on spraying a large amount of chemical pesticides to p...

Claims

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