Populus deltoidesx populus nigra PdHSP70 gene and application thereof

A gene, European and American technology, applied in the field of PdHSP70 gene, to achieve the effect of improving expression
CN103451193AInactive Publication Date: 2013-12-18DALIAN NATIONALITIES UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DALIAN NATIONALITIES UNIVERSITY
Publication Date
2013-12-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a populus deltoidesx populus nigra resistance-related gene PdHSP70 which plays an important role in the plant salt resistance process. The provided HSP gene is named as PdHSP70, and the gene has a base sequence shown in SEQ ID NO:3 of a sequence table. When the resistance-related gene PdHSP70 is constructed to an expression vector pCAMBIA1304, a promoter is added in front of a transcription initiation nucleotide, a selective marker GFP is added to authenticate and screen transgenosis vegetable cells or plants, and the expression vector carrying the resistance-related gene PdHSP70 can convert plant hosts through multiple methods and is used for cultivating salt-resistant plant varieties. The gene has wide application prospects in cultivation of salt-resistance plants.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a PdHSP70 gene from Populus deltoides×Populus nigra and its application. Background technique

[0002] European and American poplar (Populus deltoides×Populus nigra) is one of the most suitable tree species for short-rotation industrial timber intensive management in mid-latitude regions. In recent years, many excellent European and American poplar clones have been introduced into our country to create large-scale fast-growing and high-yielding forests and have achieved good economic and social benefits. But high salt etc. limit its further popularization. Therefore, when it is introduced into high-salt and water-scarce areas, screening and cultivating salt-resistant strains is a prerequisite. With the development of molecular biology, it has become an important way to solve this problem by using molecular biology techniques to study the molecular mechani...

Claims

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