Preparation method of nannochloropsis oculata polysaccharide with antioxidant activity

A technology of antioxidant activity and Nannochloropsis, which is applied in fermentation and other directions, can solve the problems of high-efficiency extraction and purification of seaweed polysaccharides, and achieve the effect of improving biological activity and extraction rate

Active Publication Date: 2021-11-02
JINING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very difficult to efficiently extract and purify seaweed polysaccharides
There are still defects in the extraction and separation technology of seaweed polysaccharides

Method used

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  • Preparation method of nannochloropsis oculata polysaccharide with antioxidant activity
  • Preparation method of nannochloropsis oculata polysaccharide with antioxidant activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation method of the Nannochloropsis polysaccharide with antioxidant activity of the present invention specifically comprises the following steps:

[0047] (1) Weigh 10 g of Nannochloropsis algae powder and add 100 mL of distilled water, place it in an extractor, and perform ultrasonic-microwave treatment for 20 minutes under the conditions of 350W ultrasonic power and 150W microwave power to obtain an extract;

[0048] (2) Add 0.3% compound enzyme to the extract under the condition of 50°C, the compound enzyme is cellulase and pectinase, cellulase: pectinase = 1:1, enzymolysis for 2 hours, to obtain liquid;

[0049] (3) The enzymatic hydrolysis solution was inactivated in a water bath at 80°C for 30 minutes, cooled and placed in a high-speed refrigerated centrifuge at 5000r / min, centrifuged at 4°C for 15 minutes, and the supernatant was taken;

[0050] (4) After the supernatant is distilled under reduced pressure, carry out alcohol precipitation with absolute...

Embodiment 2

[0065] (1) Weigh 10 g of Nannochloropsis algae powder and add 100 mL of distilled water, place it in an extractor, and perform ultrasonic-microwave treatment for 15 minutes under the conditions of 300 W ultrasonic power and 100 W microwave power to obtain an extract;

[0066] (2) Add 0.2% compound enzyme to the extract at 50°C, the compound enzyme is cellulase and pectinase, cellulase: pectinase = 1:1, enzymatic hydrolysis for 2 hours, to obtain liquid;

[0067] (3) The enzymatic hydrolysis solution was inactivated in a water bath at 80°C for 30 minutes, cooled and placed in a high-speed refrigerated centrifuge at 5000r / min, centrifuged at 4°C for 15 minutes, and the supernatant was taken;

[0068] (4) After the supernatant is distilled under reduced pressure, carry out alcohol precipitation with absolute ethanol 3 times the volume of the remaining solution, and let it stand for 10 minutes;

[0069] (5) Place the mixed solution after alcohol precipitation in a centrifuge at 5...

Embodiment 3

[0074] (1) Weigh 10 g of Nannochloropsis algae powder and add 100 mL of distilled water, place it in an extractor, and perform ultrasonic-microwave treatment for 25 minutes under the conditions of 400W ultrasonic power and 200W microwave power to obtain an extract;

[0075] (2) Add 1% compound enzyme to the extract under the condition of 50°C, the compound enzyme is cellulase and pectinase, cellulase: pectinase=1:1, enzymolysis for 2 hours, to obtain liquid;

[0076] (3) The enzymatic hydrolysis solution was inactivated in a water bath at 80°C for 30 minutes, cooled and placed in a high-speed refrigerated centrifuge at 5000r / min, centrifuged at 4°C for 15 minutes, and the supernatant was taken;

[0077] (4) After the supernatant is distilled under reduced pressure, carry out alcohol precipitation with absolute ethanol 3 times the volume of the remaining solution, and let it stand for 10 minutes;

[0078] (5) Place the mixed solution after alcohol precipitation in a centrifuge...

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Abstract

The invention belongs to the technical field of microalgae polysaccharide preparation, and particularly relates to a preparation method of nannochloropsis oculata polysaccharide with antioxidant activity. An ultrasonic-microwave-enzyme method is used for synergistically extracting the polysaccharide in the nannochloropsis oculata, and during extraction, the cavitation crushing effect of ultrasonic waves on nannochloropsis oculata powder, the high-energy effect of microwaves on the nannochloropsis oculata powder and the efficient and specific property of enzymes on cell walls of the nannochloropsis oculata powder are fully combined, so the dissolution of the polysaccharide in the nannochloropsis oculata powder is increased, and the extraction rate of the nannochloropsis oculata polysaccharide is greatly improved; the extracted nannochloropsis oculata polysaccharide is subjected to alpha-amylase modification to change the structure of the polysaccharide, so the groups and the structure of the polysaccharide subjected to shear modification on the space structure of the polysaccharide are changed, the physicochemical properties of the polysaccharide can be changed to different degrees, and the biological activity of the polysaccharide is improved; the free radical scavenging rate (-Sa/%) of the modified nannochloropsis oculata polysaccharide DPPH can be increased to 78.9% from 61.5% of the modified nannochloropsis oculata polysaccharide DPPH, and the increasing rate is 28.29%.

Description

technical field [0001] The invention belongs to the technical field of preparation of microalgae polysaccharides, and in particular relates to a preparation method of Nannochloropsis polysaccharides with antioxidant activity. Background technique [0002] Nannochloropsis (Nannochloropsis), single-celled algae, Nannochloropsis has high-quality protein, rich phycoxanthin and violaxanthin, and is rich in oil, saturated fatty acids and unsaturated fatty acids, etc. The algae has huge reserves and extremely High yield. In the prior art, there is generally extensive research on the application of Nannochloropsis in food, animal feed, health products, biodiesel, etc., but there is little research on the polysaccharide active substances contained in it. [0003] The polysaccharide contained in Nannochloropsis is seaweed polysaccharide, and its polysaccharide composition is similar to that of diatoms. Glucose accounts for 68% of the polysaccharide, and it also contains various exopol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C08B37/00
CPCC12P19/14C12P19/04C08B37/0003Y02P20/54
Inventor 彭浩绪相锦尹春光赵强王关浩
Owner JINING UNIV
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