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Totarol antibacterial nano hydrogel with whey protein as matrix and preparation method thereof

A technology of whey protein gel and nano hydrogel, which is applied in the direction of peptide/protein components, antibacterial drugs, medical preparations containing active ingredients, etc., to achieve good antibacterial effect, high biological activity, and increased elastic modulus Effect

Inactive Publication Date: 2017-04-26
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it can be seen from the hydrogel or antibacterial gel products prepared in the above-mentioned published patents that antibacterial hydrogel materials can rarely take into account the above properties

Method used

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  • Totarol antibacterial nano hydrogel with whey protein as matrix and preparation method thereof
  • Totarol antibacterial nano hydrogel with whey protein as matrix and preparation method thereof
  • Totarol antibacterial nano hydrogel with whey protein as matrix and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] (1) Preparation of whey protein gel:

[0088] a. Hydration: Add whey protein powder (15%) into deionized water, stir for 2 hours at 2000 rpm under a magnetic stirrer, and place at 4°C for 12 hours to fully hydrate.

[0089] b. Equilibration: the hydrated whey protein solution (15%) was placed at room temperature for 1 hour to equilibrate.

[0090] c. Adjust pH: adjust the pH value of the balanced whey protein solution system with 2M NaOH solution to obtain a whey protein solution with a pH value of 7.0.

[0091] d. Heating: heat the whey protein solution after pH adjustment in a constant temperature water bath at 70° C. for 10 min to form a whey protein gel.

[0092] In the preparation of whey protein gel, the percentages in brackets of each raw material are the mass content based on deionized water.

[0093](2) Totolol embedded:

[0094] a. Dissolving: Dissolving totol with absolute ethanol (10%)

[0095] b. Embedding: Add towhey protein gel (90%) according to the ...

Embodiment 2

[0106] (1) Preparation of whey protein gel:

[0107] a. Hydration: Add whey protein powder (15%) into deionized water, stir for 2 hours at 2000 rpm under a magnetic stirrer, and place at 4°C for 12 hours to fully hydrate.

[0108] b. Equilibration: the hydrated whey protein solution (15%) was placed at room temperature for 1 hour to equilibrate.

[0109] c. Adjust pH: adjust the pH value of the balanced whey protein solution system with 2M NaOH solution to obtain a whey protein solution with a pH value of 7.0.

[0110] d. Heating: heat the whey protein solution after pH adjustment in a constant temperature water bath at 70° C. for 10 min to form a whey protein gel.

[0111] In the preparation of whey protein gel, the percentages in brackets of each raw material are the mass content based on deionized water.

[0112] (2) Totolol embedded:

[0113] a. Dissolving: Dissolving totol with absolute ethanol (10%)

[0114] b. Embedding: add towhey protein gel (90%) according to the...

Embodiment 3

[0125] (1) Preparation of whey protein gel:

[0126] a. Hydration: Add whey protein powder (15%) into deionized water, stir for 2 hours at 2000 rpm under a magnetic stirrer, and place at 4°C for 12 hours to fully hydrate.

[0127] b. Equilibration: the hydrated whey protein solution (15%) was placed at room temperature for 1 hour to equilibrate.

[0128] c. Adjust pH: adjust the pH value of the balanced whey protein solution system with 2M NaOH solution to obtain a whey protein solution with a pH value of 7.0.

[0129] d. Heating: heat the whey protein solution after pH adjustment in a constant temperature water bath at 70° C. for 10 min to form a whey protein gel.

[0130] In the preparation of whey protein gel, the percentages in brackets of each raw material are the mass content based on deionized water.

[0131] (2) Totolol embedded:

[0132] a. Dissolving: Dissolving totol with absolute ethanol (10%)

[0133] b. Embedding: add towhey protein gel (90%) according to the...

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Abstract

The invention discloses a totarol antibacterial nano hydrogel with a whey protein as a matrix and a preparation method thereof, and belongs to the bacteriostatic gel biological technical field. Preparation raw materials comprise a whey protein gel, totarol and absolute ethyl alcohol. The preparation method adopts a sol-gel technique and comprises the steps of whey protein gel preparation, totarol embedding, ultrasonic treatment and standing and sterilization. According to the antibacterial nano hydrogel formula, a novel food-borne bacteriostatic agent is achieved. At the same time, the antibacterial nano hydrogel has the characteristics and advantages of the whey protein and the totarol. A whey protein polymer plays dispersion stabilizer, gel matrix and multiple roles in the antibacterial nano hydrogel system, no any crosslinking agents and initiators are added, toxic effects on a human body cannot be produced, and the use is safer and more effective; the preparation method has the advantages of simple equipment and technological process for producing the antibacterial nano hydrogel, mild conditions, low cost and easy popularization, and is suitable for industrialized production.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of antibacterial gel biology, and in particular relates to a totolol antibacterial nano hydrogel with whey protein as a matrix and a preparation method thereof. [0003] Background technique: [0004] Whey protein has been used as a raw material for more than 40 years and is one of the important high-quality protein resources. Whey protein has a high biological value (BV) and is a full-valent protein. It contains all 20 kinds of amino acids that make up the protein, and the content is higher than that of ordinary edible protein. Compared with the amino acid standard model stipulated by the FAO, its amino acid pattern is similar to that of the human body. Both can meet the needs of human AA balance. With the rise of the research on the biological function of whey protein and the development of its derivative products, it has been confirmed that whey protein contains potential biological activities suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K31/05A61K9/06A61K38/17A61P31/04
CPCA61K47/42A61K9/06A61K31/05A61K38/1709A61K2300/00
Inventor 郭明若马爽王翠娜
Owner JILIN UNIV
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