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Preparation method of degrading bacterium agent for kitchen waste treatment

A technology of food waste and degrading bacteria, which is applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of unsuitable food waste degradation, and achieve various types of molecular structures and oil degradation. High efficiency and excellent surface properties

Inactive Publication Date: 2020-07-10
中铁环境科技工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is still not suitable for the degradation of food waste with high salt content.

Method used

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  • Preparation method of degrading bacterium agent for kitchen waste treatment
  • Preparation method of degrading bacterium agent for kitchen waste treatment
  • Preparation method of degrading bacterium agent for kitchen waste treatment

Examples

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Embodiment 1

[0043] The degrading bacterial agent of the present invention, its preparation method and application thereof comprise the following steps:

[0044] (1) Bacteria activation culture

[0045] First, respectively inoculate Bacillus subtilis, Bacillus lysine, and Bacillus licheniformis into beef extract peptone liquid culture medium for activation culture, place them in a constant temperature shaker at a temperature of 30-35°C and 180-220rpm for 1-2 Days, to logarithmic growth phase, to obtain activated liquid seed solution.

[0046] The formula of beef extract peptone liquid medium is as follows: beef extract 3g, peptone 10g, yeast extract 2g, sodium chloride 5g, ultrapure water 1000mL, pH7.4-7.6.

[0047] Then Trichoderma viride, Candida glabrata and Yarrowia lipolytica strains were respectively inoculated into potato glucose medium for activation culture, placed on a constant temperature shaker at a temperature of 20-25°C and 180-220rpm for 2 -4 days, to logarithmic growth ph...

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Abstract

The invention provides a preparation method of a degrading bacterium agent for kitchen waste treatment. The degrading bacterium agent comprises Bacillus subtilis, Bacillus lysine, Bacillus licheniformis, Trichoderma viride, Candida glabrata, Yarrowia lipolytica and a biological surfactant. The method comprises the following steps: step A, activation culture; step B, domestication culture: respectively performing domestication culture on the six strains, specifically, performing step-by-step domestication culture for gradually increasing the concentration of sodium chloride for culture on eachmicroorganism; step C, liquid fermentation enlarged culture; step D, dehydrating and drying; step E, uniform mixing. The degrading bacterium agent prepared by the method can rapidly decompose proteins, grease, starch polysaccharides, cellulose, hemicellulose and other macromolecular organic matters in the kitchen waste, so that the macromolecular organic matters are rapidly stabilized, the degradation efficiency on the kitchen waste is improved, the space utilization rate of a fermentation bin is further improved, and the organic fertilizer quality of degradation products of the kitchen wasteis improved.

Description

technical field [0001] The invention relates to the field of urban garbage treatment, in particular to a preparation method of a degrading bacterial agent for kitchen waste treatment. Background technique [0002] With the rapid growth of my country's economy and the increasing level of residents' consumption, people's consumption in catering is increasing day by day, which has brought about a rapid increase in kitchen waste. In 2018, the national kitchen waste generation was about 108 million tons, with an average daily output of 292,000 tons / day, and a compound annual growth rate of over 8%. [0003] Kitchen waste is a general term for food waste and kitchen waste. Catering waste refers to the waste generated from various restaurants, hotels, hotels, and canteens of schools, enterprises and institutions during the food use process, as well as discarded leftovers, discarded edible oils and food residues, etc. Kitchen waste refers to the waste and expired waste food produc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C12N1/36C05F9/04C12R1/125C12R1/07C12R1/10C12R1/885C12R1/72C12R1/645
CPCC12N1/20C12N1/16C12N1/14C12N1/36C05F9/04Y02A40/20
Inventor 刘东斌匡文言海燕王旭伟
Owner 中铁环境科技工程有限公司
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