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Application of combination of tacrolimus and fluconazole in preparing antifungal drugs

An antifungal drug, tacrolimus technology, applied in antifungal agents, pharmaceutical formulations, medical preparations containing active ingredients, etc. active effect

Inactive Publication Date: 2014-01-22
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no large-scale quantitative study on whether the combination of FK506 and FLC can reverse the resistance of non-albicans to FLC.

Method used

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  • Application of combination of tacrolimus and fluconazole in preparing antifungal drugs
  • Application of combination of tacrolimus and fluconazole in preparing antifungal drugs
  • Application of combination of tacrolimus and fluconazole in preparing antifungal drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 FLC and FK506 joint antifungal static action assay

[0015] 1. Materials

[0016] 1.1 Experimental strains

[0017] Quality control strain: standard strain CPATCC10231 (Candida parapsilosis).

[0018] Experimental strain: clinically isolated non-albicans Candida, identified and confirmed by CHROMagar chromogenic identification medium, subcultured on solid medium. The numbers of Candida glabrata are CG1, CG2, CG3, CG4, CG5, and CG8, and the numbers of Candida krusei are CK2, CK3, CK4, CK6, CK7, CK8, CK9, and CK10.

[0019] 1.2 Drugs and reagents

[0020] FLC, Shandong Chengchuang Pharmaceutical Technology Development Co., Ltd.

[0021] FK506, National Institute for the Control of Pharmaceutical and Biological Products.

[0022] PBS (Phosphate Buffer Saline) powder, Beijing Suolaibao Technology Co., Ltd., batch number 101012.

[0023] PBS solution: Weigh 24g of PBS powder (Beijing Solaibao Technology Co., Ltd.), dissolve it in 2L of double-distilled wat...

Embodiment 2

[0062] Embodiment 2 FLC and FK506 combined antifungal dynamic action determination

[0063] 1. Materials

[0064] 1.1 Strains

[0065] The strains used in the experiment were clinically isolated Candida glabrata CG4 and Candida krusei CK4, which were identified and confirmed by CHROMagar yeast identification medium and subcultured on solid medium.

[0066] 1.2 Drugs and reagents

[0067] FLC and FK506 raw materials; dimethyl sulfoxide DMSO; PBS buffer; 0.1mol / L NaOH solution; CHROMagar chromogenic medium (France); RPMI1640 (GIBCO, the United States, containing L-glutamine, without carbonic acid Sodium hydrogen, adjust the pH to 7.0 with MOPS buffer); Yeast extract-peptone-glucose (YPD) agar medium.

[0068] 1.3 Instruments

[0069] MMM-55L constant temperature incubator (Germany); MMM-55L dry heat sterilizer (Germany); THZ-C constant temperature oscillator; HS-9041 desktop steam sterilizer (Korea); ESCO ultra-clean operating table (Korea).

[0070] 2. Content and method ...

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PUM

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Abstract

The invention discloses application of combination of tacrolimus and fluconazole in preparing antifungal drugs. According to the invention, static and dynamic combined antifungal effects of combination of tacrolimus and fluconazole to common non-albicans candida species are researched by various methods. The researches show that combination of tacrolimus and fluconazole has a remarkable synergic antifungal effect to common non-albicans candida species (candida glabrata and candida krusei). Predictably, combination of tacrolimus and fluconazole can be used for preparing antifungal drugs. According to the invention, tacrolimus and fluconazole are applied in a combined way to enhance the antimicrobial activity of fluconazole to non-albicans candida species so as to generate the synergic antifungal effect. Combination of tacrolimus and fluconazole can reverse the drug resistance of non-albicans candida species to fluconazole, thereby providing a research direction for development of new drugs and new use of old drugs.

Description

technical field [0001] The invention relates to the application of tacrolimus combined with fluconazole in the preparation of antifungal drugs. Background technique [0002] With the increasing number of cancer and AIDS patients, the widespread development of organ transplantation, catheter intubation and endoscopic techniques, the wide application of high-efficiency broad-spectrum antibiotics, FK506 and hormones, the incidence of deep fungal infections in humans has increased year by year, and fungi have become important pathogens of nosocomial infections. Candida albicans is a common isolate in clinical fungal infections, and fluconazole (FLC) is effective against it. However, with the widespread clinical application of FLC, non-Candida albicans species that are not sensitive to it, such as Candida glabrata (CG), Candida tropicalis, and Candida krusei (CK) ), Candida parapsilosis, etc., the isolation rate gradually increased, and more and more non-albicans were isolated ...

Claims

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Application Information

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IPC IPC(8): A61K31/436A61P31/10A61K31/4196
Inventor 孙淑娟李辉鲁春燕杨久丽李敏
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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