Mehtod for preparing natural 4-vinyl guaiacol by biological transformation

Abstract

The invention relates to a method for preparing natural 4-vinyl guaiacol by biological transformation. According to the method disclosed by the invention, Bacillussp. HY3CCTCCM2012247 is taken as a biocatalyst, natural ferulic acid is taken as a substrate, and the natural 4-vinyl guaiacol is prepared by microbial transformation. The production process comprises the following steps of: (1) performing slant culture; (2) preparing a seed culture solution; (3) culturing transformation cells; and (4) transforming. The method disclosed by the invention belongs to the technical field of biology, thenatural 4-vinyl guaiacol can be produced with high transformation rate by adopting the method, and the method can be used in the fields of tobacco, wine, foods and the like and further has great application prospects.

Description

technical field

[0001] The present invention relates to the method for biotransformation preparation natural 4-vinyl guaiacol, specifically, especially relate to using a strain of bacillus Bacillus sp. HY3 CCTCC M 2012247 Method for preparing natural 4-vinylguaiacol by converting ferulic acid. Background technique

[0002] 4-vinylguaiacol (4-vinylguaiacol) is also known as 2-methoxy-4-vinylphenol, p-vinylguaiacol, and its molecular formula is C 9 h 10 o 2 , density 1.11×103 kg / m 3 , melting point 26°C~29°C, boiling point 100°C / 667Pa. It is a colorless or light yellow oily liquid at normal temperature and pressure. It has a fermented aroma and a slightly sweet taste. It is the main aroma component that determines the quality of food such as wine, soy sauce, tea, coffee, and cheese. It is also used in the synthesis of daily chemicals, medicine, and essence. One of the more commonly used high-grade spices in other industries. Natural 4-vinylguaiacol mainly exists in the ...