Mehtod for preparing natural 4-vinyl guaiacol by biological transformation

A technology of guaiacol and biotransformation, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as low yield and complicated process route of 4-vinylguaiacol, and achieve The effect of high product concentration, product environmental friendliness and mild reaction conditions

Active Publication Date: 2012-11-14
ENERGY RES INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the increasing demand for natural products year by year, and the problems of complicated chemical synthesis of 4-vinyl guaiacol and low yield,...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Bacillus Bacillus Sp. HY3 CCTCC M 2012247 cells were inserted into the transformation medium with an inoculum of 6% volume percentage after 15 hours of seed culture, and 0.2 g / L ferulic acid was added after 20 hours of cell culture, and 120 rpm at 30°C Shake culture / min. After 10 hours, samples were taken to determine the concentration of 4-vinylguaiacol.

[0020] The bacillus that the present invention relates to Bacillus sp. HY3 CCTCC M 2012247 converts ferulic acid to prepare natural 4-vinylguaiacol, and the detailed steps involved are as follows:

[0021] (1) Slant culture: Bacillus Bacillus sp. HY3 CCTCC M 2012247 was inoculated on LB solid medium supplemented with 1% glucose by mass and volume ratio, and cultured statically for 20 hours at 30°C to obtain a mature slant culture;

[0022] (2) Preparation of seed culture solution: use the slant culture obtained in step (1) to inoculate into LB liquid medium with 1% glucose in mass volume ratio, and culture at ...

Embodiment 2

[0028] Bacillus Bacillus Sp. HY3 CCTCC M 2012247 cells were inserted into the transformation medium with an inoculum of 6% volume percentage after 15 hours of seed culture, and 0.5 g / L ferulic acid was added after 20 hours of cell culture, and 120 rpm at 30°C Shake culture / min. Wherein the substratum formulation used for seed culture and transformed cell culture is the same as that of Example 1.

[0029] After 10 hours, a sample was taken to determine that the concentration of 4-vinylguaiacol was 0.366 g / L, and the molar conversion rate was 94.7%.

Embodiment 3

[0031] Bacillus Bacillus sp. HY3 CCTCC M 2012247 cells were inserted into LB liquid medium with an inoculation volume of 6% by volume after 15 hours of seed culture, and 1 g / L ferulic acid was added after 20 hours of cell culture. Shake the culture at rpm. Wherein the substratum formulation used for seed culture and transformed cell culture is the same as that of Example 1.

[0032] After 10 hours, a sample was taken to determine that the concentration of 4-vinylguaiacol was 0.710 g / L, and the molar conversion rate was 91.8%.

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PUM

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Abstract

The invention relates to a method for preparing natural 4-vinyl guaiacol by biological transformation. According to the method disclosed by the invention, Bacillussp. HY3CCTCCM2012247 is taken as a biocatalyst, natural ferulic acid is taken as a substrate, and the natural 4-vinyl guaiacol is prepared by microbial transformation. The production process comprises the following steps of: (1) performing slant culture; (2) preparing a seed culture solution; (3) culturing transformation cells; and (4) transforming. The method disclosed by the invention belongs to the technical field of biology, thenatural 4-vinyl guaiacol can be produced with high transformation rate by adopting the method, and the method can be used in the fields of tobacco, wine, foods and the like and further has great application prospects.

Description

technical field [0001] The present invention relates to the method for biotransformation preparation natural 4-vinyl guaiacol, specifically, especially relate to using a strain of bacillus Bacillus sp. HY3 CCTCC M 2012247 Method for preparing natural 4-vinylguaiacol by converting ferulic acid. Background technique [0002] 4-vinylguaiacol (4-vinylguaiacol) is also known as 2-methoxy-4-vinylphenol, p-vinylguaiacol, and its molecular formula is C 9 h 10 o 2 , density 1.11×103 kg / m 3 , melting point 26°C~29°C, boiling point 100°C / 667Pa. It is a colorless or light yellow oily liquid at normal temperature and pressure. It has a fermented aroma and a slightly sweet taste. It is the main aroma component that determines the quality of food such as wine, soy sauce, tea, coffee, and cheese. It is also used in the synthesis of daily chemicals, medicine, and essence. One of the more commonly used high-grade spices in other industries. Natural 4-vinylguaiacol mainly exists in the ...

Claims

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Application Information

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IPC IPC(8): C12P7/22C12N1/20C12R1/07
Inventor 华栋梁梁晓辉张晓东张杰许海朋李岩许敏
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
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