82 results about "Bio transformation" patented technology

The invention relates to processes that efficiently convert carbon-containing materials, such as biomass, into products in such a manner that the energy, carbon, and mass content of the materials are efficiently transferred into such products. Such methods include converting the materials into at least one intermediate by a biological conversion process and at least one intermediate by a thermochemical conversion process and reacting the intermediates to form the product. Such methods have a chemical energy efficiency to produce the product that is greater than the chemical energy efficiency of a solely biological conversion process to produce the product and that is greater than the chemical energy efficiency of a process in which all of the material is initially subjected to a thermochemical conversion step as part of the process to produce the product.

The invention relates to a method for improving the transformation efficiency of (S)-phenyl glycol by coupling glucose-6-phosphate dehydrogenase and (S)-carbonyl reductase, belonging to the technical field of biological catalytic asymmetric transformation. The invention provides the method for preparing the (S)-phenyl glycol by recombinant Pichia pastoris with CGMCCNo.4198 and 2-hydroxyacetophenone catalyzed by the recombinant Pichia pastoris with CGMCCNo.4198 in multiple batches. In the invention, the Saccharomyces cerevisiae glucose-6-phosphate dehydrogenase gene and the Candida parapsilosis (S)-carbonyl reductase gene are simultaneously integrated into the Pichia pastoris genome, under the participation of 5% (w/v) glucose, the whole cells are reused for 10 batches, and the optical purity and yield of the (S)-phenyl glycol prepared by asymmetric transformation are maintained at 100% and higher than 85% all the time. The recombinant strain improves the batch stability of full cell transformation of the (S)-phenyl glycol by polygene coexpression, which well relieves the limit of coenzyme regenerative cycle in a biological transformation reaction; and simultaneously an efficient approach for preparing the (S)-phenyl glycol industrially in multiple batches at low cost is provided.

The invention belongs to the technical field of bio-pharmaceuticals, and in particular relates to a method for producing a dexamethasone epoxide hydrolysate through one-step microbial fermentation and transformation. According to the method, with 16a-methyl-17[alpha],21-dihydroxypregna-4-ene-9,11-epoxy-3,20-dione-21 acetate as a substrate, the dexamethasone epoxide hydrolysate is directly produced mainly by conducting mixed fermentation by virtue of arthrobacter simplex and bacillus megatherium. In comparison with the prior art, the method provided by the invention, on the basis of a synergistic effect between mixed strains, integrally combines two steps of reactions, namely dehydrogenation and hydrolysis, so that the finished dexamethasone epoxide hydrolysate is directly obtained; and the product has the advantages of being short in production cycle, high in bio-transformation rate, high in yield, environment-friendly and low in cost.

The invention relates to a method for preparing natural 4-vinyl guaiacol by biological transformation. According to the method disclosed by the invention, Bacillussp. HY3CCTCCM2012247 is taken as a biocatalyst, natural ferulic acid is taken as a substrate, and the natural 4-vinyl guaiacol is prepared by microbial transformation. The production process comprises the following steps of: (1) performing slant culture; (2) preparing a seed culture solution; (3) culturing transformation cells; and (4) transforming. The method disclosed by the invention belongs to the technical field of biology, thenatural 4-vinyl guaiacol can be produced with high transformation rate by adopting the method, and the method can be used in the fields of tobacco, wine, foods and the like and further has great application prospects.

The invention discloses a corynebacterium glutamicum engineering bacterium for anaerobic conversion to produce succinic acid, and a building method and application thereof, and belongs to the field of genetic engineering. A pyruvate carboxylase gene of the corynebacterium glutamicum and phosphoenolpyruvate carboxylase from escherichia coli are cloned to corynebacterium glutamicum (ATCC13032); a lactate dehydrogenase gene of the corynebacterium glutamicum is knocked out in a homologous recombination manner. By adopting the lactic dehydrogenase-defective corynebacterium glutamicum for coexpression of a carboxylase gene, anaerobic production of succinic acid is carried out in a cell reutilization manner, so that the yield of succinic acid can be greatly improved; the yield can be up to 75g/L; the conversion rate of saccharic acid is 75%; the corynebacterium glutamicum engineering bacterium has a good application prospect; a fermentation model, especially a fermentation model for cell reutilization is built according to the optimum condition for biological transformation of succinic acid; the acid-production performance in repeated batch transformation process of cells can be basically kept stable.

The present invention relates to a biocatalyst displayed on spore or virus surface and a method of bioconversion using the same, in particular to a method of bioconversion using a biocatalyst, which comprises the steps of: (a) preparing a vector for spore surface display comprising a gene construct containing a gene encoding a display motif and a gene encoding the biocatalyst, wherein, when expressed, the gene construct expresses the display motif and the biocatalyst in a fusion form and the biocatalyst is displayed on a spore surface; (b) transforming a host cell with the vector for spore surface display; (c) displaying the biocatalyst on the spore surface of the host cell; (d) recovering the spore displaying on its surface the biocatalyst; and (e) performing the bioconversion reaction using the spore displaying on its surface the biocatalyst, and a biocatalyst.

A flow wood processing extracting lignin from woody plant material and converting delignified cellulosic residue to crude bio-oils, that removes toxic preservative chemicals from waste timber, and provides conversion to useful or nontoxic forms. Wood is chipped and fed into a lignin extractor using ethanol at high temperatures to dissolve the lignin with counter current material contactors, heat exchangers, and a computer control system. Most of preservative chemicals likely precipitate out at this stage as a heavy sludge that can be removed. Ethanol containing dissolved lignin is removed from the lignin extractor, the dissolved lignin recovered, the ethanol recycled into the lignin extractor, and residual heat returned to the process. Delignified cellulosic pulp is removed from the lignin extractor and subjected to a milling to convert into a smooth sludge for entry to a bio-convertor by a super critical water process. The residue is prepared as a high phosphate fertilizer.

The present invention belongs to the technical field of perfume for tobacco, and particularly relates to endophyte bio-transformed raisin extract and preparation and application in flavoring thereof. The method is implemented as follows: (1) separating and purifying endophyte; (2) culturing endophyte; (3) extracting and refining raisin; and (4) bio-transforming raisin extract with the endophyte to obtain bio-transformed raisin extract. The method of the present invention has simple technological process and low cost, and the obtained bio-transformed raisin extract can be directly used for theflavoring and casing of cigarettes. The perfume of bio-transformed raisin extract has a unique fresh and sweet smell, and can lower stimulation after being added to cigarettes. The perfume of bio-transformed raisin extract has a strong fresh and sweet smell as well as a strong toasting smell.

The invention relates to biofermentation field, especially relates to the submerged fermentation craft of the drumstick quern which appended balsam pear, and the fermented product of the craft. The characteristic is: take drumstick mushroom as start strain, do liquid shaking culture, liquid shaking enlargement culture, stair seed culture and the ferment culture which is added with balsam pear. The invention solves the problem of the drumstick mushroom's cosmically fluid culture, greatly improves the fermentation liquor activity because the traditional medicine balsam pear in the fermentation culture medium, together with the drumstick mushroom have done biological transformation, it has important sense to the drumstick mushroom's industry exploitation. The fermentation liquor is tremendous or brown liquid, and the mycelium is tremendous. The dry weight of the fermentation liquor mycelium is 7 to 10 gram per liter, and the restrain rate to extraneous non-enzyme glycosylation of the fermentation liquor is up to 93 to 98 percent.

The invention relates to Lactobacillussp.W2. The preservation number of the Lactobacillussp.W2 is CCTCCM209318. The invention also relates to application of the Lactobacillussp.W2 in biologically transforming phenylpyruvic acid substrate and extracting phenyllactic acid from fermentation solution by using ethyl acetate. The Lactobacillussp.W2 is from the traditional fermented food, belongs to an acknowledged safe strain and can be applied to food; the strain grows well on an MRS culture medium and is easily cultured and stored; and the content of the phenyllactic acid obtained by fermentation can reach 1.038 grams per liter, and the phenyllactic acid has strong capability of inhibiting putrefactive bacteria in the food.

The invention relates to a 1, 3-propanediol oxidoreductase isozyme mutated gene and application thereof. By utilizing error-prone PCR technique-mediated random mutation, the1, 3-propanediol oxidoreductase isozyme mutated gene is obtained by screening. The codon GAC of the No. 99 aspartic acid in the gene is mutated as the codon CAA of glutamine; the codon AAC of the No. 147 asparaginate is mutated as histidine CAC; a nucleotide sequence of the mutated gene is SEQ ID No.1, and is capable of being encoded as 1, 3-propanediol oxidoreductase mutated isozyme. The invention creates 1, 3-propanedioloxidoreductase mutated isozyme that has relatively high catalytic activity to 3-Hydroxypropionaldehyde and can catalyze the 3-hydroxypropionaldehyde to produce chemical raw material 1, 3-propanediol.Compared with the 1, 3-propanediol reducase isozyme, the activity of one of the tested cases is increased by 4 to 4.6 times, which provides broad application prospect for bio-transformed production chemical material 1, 3-propanediol.

The invention discloses a fermentation method for a natural perfume gamma-decalactone, Namely, under the conditions that ((i)saccharomyces cerevisiae (/i)) CGMCCNo.2.630 is used as a strain, and castor oil is used as a substrate, porcine pancreatic lipase is added, a natural perfume gamma-decalactone is prepared through a batch fermentation or fed-batch fermentation method in an aerobic environment. The fermentation method for a natural perfume gamma-decalactone disclosed by the invention reduces the cycle of transforming castor oil into natural gamma-decalactone and improves the ratio of transforming castor oil into natural gamma-decalactone and the yield of final products gamma-decalactone, wherein the biological transformation cycle of castor oil is 12-30h, the transformation rate of castor oil can reach 95%, and the biosynthesis efficiency of gamma-decalactone can reach 94.9%, especially in a 5L fermentation tank, the final yield of gamma-decalactone prepared through batch fermentation can reach 4.81g/L, and the final yield of gamma-decalactone prepared through fed-batch fermentation can reach 6.50g/L.

The invention discloses a biological transformation and synthesis method of rubescensin. The synthesis method comprises the following steps of: firstly, preparing a solid culture medium and a liquid culture medium; then, sterilizing and then inoculating the leaves, leaf buds or rhizome shucks of rabdosia rubescens into the prepared solid culture medium for culturing; transferring the callus obtained by culturing into the liquid culture medium for culturing; extracting the callus cultured in the liquid culture medium by adopting an organic solvent; and removing the organic solvent after extracting to obtain a rubescensin product (kaurene type diterpenoid compound). In the invention, the technical scheme for culturing and preparing rubescensin is free from the restriction of natural resources and is favorable for protecting the ecological environment. The technical scheme disclosed by the invention is easy to realize industrialized production, has the advantages of shorter culture time and higher product yield, lowers the production cost, and has obvious economic and social benefits.

The invention belongs to the application field of biology engineering technology, and particularly relates to a method of immobilizating gibberella CA3-1(Gibberella intermedia) with a preserved number of CGMCC No.4903 and using the gibberella for biological transformation to prepare nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid includes: immobilizing the gibberella CA3-1, adding a proper amount of 3-cyanopyridine, and choosing an optimum reaction condition for immobilizing thalluses to obtain a product which is the nicotinic acid. The method of immobilizing the gibberella and using the gibberella for the biological transformation to prepare the nicotinic acid strengthens tolerance of the thalluses to substrates after the thalluses are immobilized, improves production capacity of unit thalluses, simplifies separation processes of products greatly, increases reutilization times obviously, and provides a foundation for achieving technology of performing a biological transformation method by using the gibberella to prepare the nicotinic acid.

The invention discloses a genetically engineered bacterium for producing protocatechuic acid (3, 4-dihydroxybenzoic acid) by using phenol as raw material and a construction method thereof. The engineering strain contains p-hydroxybenzoic acid decarboxylase gene yclBCD and hydroxylase gene pobA, phenol is used as raw material, phenol is carboxylated to obtain p-hydroxybenzoic acid by biological transformation, and then 3-hydroxybenzoic acid is oxidized to obtain protocatechuic acid (3, 4-dihydroxybenzoic acid). Compared with the traditional methods such as plant extraction and chemical synthesis, this method is convenient and quick, and will not pollute the environment, and can reasonably utilize phenol to save energy and protect the environment. It is of great significance to the production of protocatechuic acid.

The invention discloses a method for extracting ephedrine, which uses a semi-bionic-biological enzyme extraction method, which overcomes the shortcomings of the semi-bionic method under normal pressure, such as high extraction temperature and difficulty in separation and purification. The addition of enzymes is beneficial to the leaching of active substances and biological Transformation, on the premise of not destroying the original ingredients, more effectively extract the ephedrine in the ephedra.

The invention provides a polygonatum canaliculatum plant extracting solution, fermented yoghourt and a preparation method thereof, and relates to the technical field of microbial fermentation and biological transformation. The polygonatum canaliculatum plant extracting solution is mainly prepared from polygonatum canaliculatum by a heating water extraction method, and the fermented yoghourt prepared from the polygonatum canaliculatum plant extracting solution, fresh milk, sucrose and a compound fermentation strain has the effects of nourishing yin, reinforcing the spleen, moistening the lung and promoting the production of the body fluid, and has good health care effects on symptoms such as deficiency of body after disease, inappetence and hypoimmunity. The fermented product reserves the nutrient components of the original yoghourt, and simultaneously has partial effects of polygonatum canaliculatum, thereby well combining the health care effects of yoghourt and polygonatum canaliculatum. Due to good color, good mouthfeel and good flavor of the milk curd, the fermented product alleviates the problem that most of the existing health products have poor flavoring effects and poor acceptability of consumers.

The invention belongs to the field of biological fermentation and in particular relates to scutellaria baicalensis and lactic acid bacteria yeasts, cosmetics containing yeasts, a preparation method and application. According to the technical problem to be solved, the biological transformation theory is taken as the guidance, a product with the effects of whitening, removing acnes and controlling oil is provided by adopting a liquid fermentation system of scutellaria baicalensis and lactic acid bacteria. The scutellaria baicalensis and lactic acid bacteria yeast refers to a fermented liquid mixture obtained by the step of fermenting a culture medium consisting of scutellaria baicalensis and water with lactic acid bacteria, wherein the culture medium comprises 5-10 weight percent of scutellaria baicalensis based on the weight of a crude drug, and the inoculation amount of the lactic acid bacteria is 1.0*10<4> to 1.0*10<8>cfu/ml.

The invention which belongs to the technical field of biological engineering relates to a process for o-cresol compound conversion with a biological method. The process is characterized in that: a first step biological conversion reaction is carried out to obtain a product 3-methycatechol with conditions that a dry weight of whole cells of phenol hydroxylase gene engineering bacteria PH-IND in a reaction solution is from 20 to 40 g/L, a concentration of o-cresol is from 50 to 700 mg/L, the concentration of glucose is from 5 to 40 mmol/L, a temperature is from 20 to 40 DEG C, a shaker speed iscontrolled from 50 to 200 r/min, and an oscillation reaction period is from 10 to 240 min; and a second step biological conversion reaction is carried out with the conditions that the dry weight of the whole cells of biphenyl dioxygenase gene engineering bacteria BphC-LA-4 which are added in the reaction solution is from 20 to 40 g/L, the shaker speed is controlled from 50 to 200 r/min, and the oscillation reaction period is from 5 to 50 min. The method has the advantages of mild reaction condition, single product and simple extraction step, allows the product to be recycled, and is suitable for industrial production and application.

The invention provides a tremella fuciformis biotransformation composition of maca and Chinese yam and a preparation method of the composition. According to the invention, a unique combination of macapowder, Chinese yam powder and soybean powder is employed for the first time, and a deep fermentation and bio-transformation technology using tremella fuciformis is utilized. Through reasonable matching of tremella fuciformis, maca and Chinese yam, and technologies of adjusting of the acidity and alkalinity step by step and temperature-raising heat-preservation conversion, the tremella fuciformisbiotransformation composition of maca and Chinese yam is manufactured, and the composition has various health-care functions. The invention not only provides a bioconversion extraction method for bioactive substances in Maca and Chinese yam, but also provides a method for improving tremella fuciformis polysaccharides. Animal experiments prove that the tremella fuciformis biotransformation composition of maca and Chinese yam is produced by the method disclosed by the present invention has functions of improving lung fibrosis function, resisting fatigue and the like.

The present invention relates to a method for degrading DNA in a sample obtained by microbial fermentation or bio-transformation, comprising treating the sample with a combination of increased temperature and low pH. It also relates to a method for releasing DNA from a microbial cell, comprising incubating the microbial cell at a temperature of 45° C. to 55° C. for two to ten hours. Finally, the present invention provides a method for producing a product, comprising a step of releasing DNA from a microbial cell and degrading said DNA.

The invention provides a method for preparing 4-androstenedione through phytosterin. The method comprises the following steps: 1, culturing a branched bacillus strain, a seed slant solid culture medium and sterile water which are obtained through automatic separation and screening at constant temperature for 5-6 days to obtain a seed; 2, dissolving a first-level seed culture medium into water, transferring the seed obtained from the step 1 into the first-level seed culture medium, and placing on a shaking table for 72 hours to obtain a first-level seed; 3, dissolving a second-level seed culture medium into the water, transferring the first-level seed obtained from the step 2 into the second-level seed culture medium, and reacting for 48 hours to obtain a second-level strain; 4, respectively placing the phytosterin, bean oil and tween into a fermentation tank, adding a biological transformation culture medium, transferring the second-stage strain obtained from the step 3 into the biological transformation culture medium till no phytosterin residues are detected to exist, heating to 90 DEG C, standing for 3 hours, and layering to obtain a water layer; 5, extracting an oil layer by using an organic solvent, carrying out reduced pressure concentration to a quarter of the volume of the organic solvent, cooling to 5 DEG C, and filtering to obtain a crude product; and recrystallizing by using methanol to obtain a 4-androstenedione finished product.