37 results about "Bio transformation" patented technology

The invention discloses a decomposition preparation for coniferous forest plant residues and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composting preparation for the coniferous forest plant residues comprises bacillus subtilis, bacillus licheniformis and Maribacter thermophilus, the coniferous forest plant residues, fresh chicken manure and peanut shells or corn stigma are used as main raw materials for composting, the aerobic bacillus mainly plays a key role in decomposing lignocellulose and releasing nutrients, and the aerobic bacillus mainly plays a key role in decomposing the lignocellulose and releasing the nutrients; the three strains are used as functional auxiliary strains, the three strains have a synergistic effect and jointly promote efficient and directional decomposition conversion of the refractory coniferous forest residues, and finally the decomposition preparation which is rich in active functional groups, has a porous structure and is high in adsorption capacity is prepared. The invention provides an innovative biological conversion path for high-value resource utilization of the coniferous forest waste.

The application discloses a Yarrowia lipolytica strain with high yield of gamma-decalactone and / or delta-decalactone and application thereof, and belongs to the technical field of biotechnology. The name of the strain is Yarrowia lipolytica Y-1, and the preservation number is GDMCC No: 67810. The strain Y-1 can be directly used for biological transformation to cooperatively and efficiently prepare gamma-decalactone and delta-decalactone with castor oil acid as a substrate. The strain has three outstanding advantages: ① yield advantage; ② tolerance advantage; and ③ application advantage. The biological transformation method has the advantages of mild reaction condition, low cost, product meeting natural perfume standards, environmental friendliness and the like.

The invention belongs to the technical field of biology, and particularly relates to a Sardinia clostridium 7alpha-hydroxysteroid dehydrogenase mutant S161G. The amino acid sequence of the mutant S161G is as shown in SEQ ID NO: 2, and the amino acid sequence of the mutant S161G is obtained by mutating Ser at the 161st site of a wild type sequence SEQ ID NO: 1 into Gly. The catalytic efficiency of the mutant S161G on substrates NADP < + > and TCDCA is 3.82 times that of a wild type. The mutant S161G provided by the invention has huge application potential in the industrial production process of obtaining TUDCA or UDCA through biotransformation.

The invention belongs to the field of food science and engineering, and discloses a preparation method of an active medicinal and edible composite nutrient AMCN with an anti-aging effect, and the preparation method comprises the following steps: putting a ganoderma lucidum monoclonal strain into a liquid culture medium for controllable culture to obtain supernate with beta-glucosidase activity of over 100mU / mL; and adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder, carrying out enzymatic biotransformation, carrying out lactobacillus plantarum fermentation, adding a freeze-drying protective additive, carrying out freeze-drying, grinding to obtain the active medicinal and edible composite nutrient AMCN, and storing the finished product in a dry and dark place. The active medicinal and edible composite nutrient can be used for preparing anti-aging functional foods or nutritional health-care products. A D-galactose induced zebrafish aging model verifies that the active medicinal and edible composite nutrient prepared by the application plays an anti-aging role by relieving oxidative stress, inhibiting cell aging and maintaining telomere stability, can reduce beta-galactosidase activity and intracellular ROS (reactive oxygen species) level, improves telomerase protein concentration, and has a good anti-aging effect. And the telomere length can be effectively recovered by medium and high concentrations.

The invention relates to the technical field of breeding pollution treatment, and particularly discloses a biotransformation treatment device for resource utilization of livestock and poultry breeding manure, which comprises a pretreatment mechanism, a waste treatment mechanism, a waste treatment mechanism and a waste treatment mechanism, and is characterized in that the pretreatment mechanism comprises a spiral conveying assembly and a crushing assembly; the biological reaction mechanism comprises a tower body, a plurality of filler layers and an aeration assembly, the filler layers are arranged at intervals in the height direction of the tower body, and the aeration assembly comprises an air supply part, an air supply pipe network communicated with the output end of the air supply part and an aeration pipe connected to the air supply pipe network. The air outlet end of the aeration pipe faces the filler layer; the separation mechanism is used for carrying out solid-liquid separation on the feces; the recycling mechanism comprises a biogas recycling assembly and an organic fertilizer recycling assembly. According to the application, continuous operation from pretreatment to resource recovery of the feces is realized, and no intermediate link leakage exists; the solid-state isolate is converted into organic fertilizer, the liquid-state isolate is used for recycling biogas, resources are completely recycled, secondary pollution is avoided, and the requirement for green breeding is met.

The invention relates to an artificial domestication and cultivation method for Mongolian white mushroom based on micro-ecological simulation, and innovatively provides a micro-ecological simulation cultivation strategy aiming at the core technical bottlenecks that hyphae of Mongolian white mushroom grow extremely slowly, a conventional culture medium does not eat materials and fruiting is difficult. The method is characterized in that specific soil leaching liquor is prepared from mongolian white mushroom circle rhizosphere soil and added into a mother strain culture medium, and key microbial flora and growth factors for promoting hypha growth are successfully introduced; meanwhile, a special stock seed and a cultivation material which take fermented sheep manure and pasture root system granules as main materials are designed, and nutrients and physical structures of the original grassland ecology are accurately simulated. According to the method, the problem of hypha growth stagnation is thoroughly solved, the culture periods of mother seeds, stock seeds and cultivated seeds are remarkably shortened to 14 days, 22 days and 46 days from conventional 30 days or more, 40 days or more and 66 days or more, stable fruiting is successfully achieved, and the biotransformation rate is increased by 11.2% or more compared with that of a conventional method.

The application provides a kind of simulation new pollutant intermittent input mode under the action of groundwater soluble N2O biological conversion layered sampling experimental device and continuous dynamic monitoring method, belongs to groundwater pollution control experimental technical field.Simulation new pollutant intermittent input mode under the action of groundwater soluble N2O biological conversion layered sampling experimental device mainly includes groundwater simulation experimental column, liquid inlet and mixing system, microelectrode monitoring system and layered sampling component;The experimental column is arranged in parallel, and is connected with liquid inlet and mixing system by peristaltic pump, realizes soluble N2O stable liquid inlet, and simulates different input modes by regulating and controlling new pollutant input concentration and frequency;The layered sampling component is used for pore water and microbial sample collection, and microelectrode is used for continuous monitoring of soluble N2O.The application can simulate different input modes of new pollutants in groundwater, realize layered sampling and continuous monitoring, and is suitable for studying the influence of new pollutants on the biological conversion process of soluble N2O in groundwater.

The present disclosure relates to a pectin-containing wastewater treatment method and a treatment device, the method comprising: inputting first pectin wastewater into a biological conversion tank, and performing biological conversion treatment on pectin in the first pectin wastewater based on a pectin-cellulose degradation mixed bacteria to obtain second pectin wastewater containing small molecular structure pectin; wherein the pectin-cellulose degradation mixed bacteria is a mixed bacterial population capable of simultaneously degrading pectin and cellulose, and the biological conversion treatment process is used to change the pectin structure without affecting the chemical oxygen demand corresponding to the first pectin wastewater; inputting the second pectin wastewater into a primary or multi-stage anaerobic reactor for anaerobic decomposition treatment to obtain wastewater with reduced organic matter concentration after pectin decomposition and biogas; and the biogas is used for input into a power supply end or an energy storage end for recycling. In the wastewater treatment process, in-situ treatment and relatively sufficient degradation of pectin are achieved, and the treatment load and treatment efficiency are improved.

The present application belongs to the technical field of animal feed, and discloses a feed based on fermented raw materials and a preparation method thereof. The feed is made of main fermented raw materials, nutritional adjuvants, fermentation agents and functional additives. The preparation method comprises the following steps: S1, raw material pretreatment and mixing; S2, inoculation and fermentation; and S3, post-treatment and finished product. The present application has the following beneficial effects: dual improvement of nutrition and function, deep biological transformation makes the feed more digestible, and endogenously generates various active substances, promotes growth and improves immunity; stable and controllable process, clear strain combination and two-stage fermentation parameters ensure the stability and repeatability of product quality, suitable for large-scale production; safe and environmentally friendly, no chemical addition in the production process, detoxification and improvement are achieved through biological fermentation, and the product is green and safe; wide application, through adjusting the main material ratio and adjuvants, a series of products suitable for pigs, poultry, ruminants and aquatic animals can be derived, and the adaptability is strong.

The invention discloses high-activity cherry powder based on probiotic fermentation and biological enzyme synergistic treatment and a preparation method of the high-activity cherry powder. The preparation method comprises the following steps: S1, material selection and pretreatment; s2, specific probiotic strain fermentation treatment: performing low-temperature fermentation by adopting a screened lactic acid bacteria and saccharomycetes mixed strain to promote biotransformation and release of active ingredients; s3, biological enzymolysis: adding compound pectinase and beta-glucosidase to carry out synergistic enzymolysis, so as to improve the bioavailability of the active polyphenol; s4, combined drying: firstly, quickly dehydrating the cherry pulp to intermediate water content by adopting microwave vacuum drying, and then preparing into powder by spray drying; and S5, carrying out microencapsulation and packaging. The core of the method is that through a probiotic fermentation and biological enzyme synergistic treatment technology, the bioavailability and the stability of the cherry are improved through biological conversion while heat-sensitive nutritional ingredients such as procyanidine and vitamin C in the cherry are reserved.

The invention discloses a coupling system of a methanization unit and a biological transformation unit of organic matter by insect larvae, said methanization unit being adapted to receive as input a first stream of organic matter, comprising: at least one bypass device connected to at least one subset of said methanization unit, at least one control device, configured to command said at least one bypass device to carry out at least one operation of: i) taking at least a part of said stream of matter treated by said methanization unit, taken from at least one subset of said methanization unit, to constitute a second stream and supply it as input to the biological transformation unit;ii) taking a flow of material from the output of the biological transformation unit to supply it as input to at least a subset of said methanization unit through a reinjection device. [Fig 3];

The invention relates to the technical field of extraction processes, in particular to a method for extracting flavone in pericarpium citri reticulatae under the assistance of fermentation liquor. According to the invention, the synergistic effect of mixed strains is adopted, the bacillus subtilis is utilized to decompose organic matters and generate enzymes, and the saccharomycetes is utilized to metabolize, ferment and promote transformation, so that cell walls of the dried orange peel are more comprehensively destroyed through cellulase, and a biotransformation path of flavonoid substances is activated, thereby improving the extraction efficiency and the content of active ingredients; according to the method disclosed by the invention, optimization is carried out through a step-by-step process, efficient release and separation of flavonoid components are realized through pH regulation and control, cellulose enzymolysis and ethanol extraction after fermentation, and limitation of a traditional extraction process is avoided; the specific safety and stability are guaranteed, the active structure of flavone is reserved to the maximum extent through low-temperature enzymolysis and vacuum concentration processes, meanwhile, damage to heat-sensitive components is reduced, and the stability and safety of the raw materials are improved.

The invention provides a skin anti-aging and firming composition, oil cream and a preparation method thereof, and belongs to the field of medical, dental or cosmetic preparations. The composition comprises a palmaria palmata and inula flower extract, a vaccinium macrocarpum fruit extract, a medicine root extract and the palmaria palmata and inula flower extract, palmaria palmata and inula flower serve as fermentation substrates, aerobic fermentation is conducted through aspergillus niger, and anaerobic fermentation is conducted through pediococcus pentosaceus; and purifying to obtain the product. According to the palmaria palmata inula flower fermentation extract provided by the invention, through sequential fermentation of aspergillus niger and pediococcus pentosaceus, deep biotransformation of a plant matrix is realized. The extract has the beneficial effects that the moisturizing and repairing characteristics of palmaria palmata and the anti-inflammatory and anti-oxidation characteristics of inula flower are fused, new active matters such as lactic acid and small molecule peptide are generated through microbial conversion, and the comprehensive effect is obviously superior to that of a traditional extract; macromolecules are converted into micromolecules easier to absorb through fermentation, bioavailability is improved, the pH value of the product is close to the skin, and the product is mild and low in irritation.

The application provides a method for solid-state fermentation of poria cocos by purple monascus CSU-M183 and enzymes, and the method is characterized in that: the method is a biological transformation method, and the traditional Chinese medicine poria cocos is used as raw material, and the poria cocos is subjected to bidirectional solid-state fermentation by enzyme pretreatment and purple monascus CSU-M183 to improve the yield of Monacolin K, water-soluble polysaccharides and triterpenoid compounds, and no citrinin is generated. The method improves the efficient utilization of poria cocos resources, increases the added value of the poria cocos, provides a new processing technology guidance for poria cocos products, and provides a method for saving resources, reducing cost and high safety for high-value comprehensive utilization of the traditional Chinese medicine poria cocos.

The application provides a kind of Lactiplantibacillus plantarum and its application in increasing the content of curcumin in fermentation product.The Lactiplantibacillus plantarum of the application has been preserved in Guangdong Microbial Culture Collection Center on November 13, 2025, the address of preservation is No. 59 Building, 5th Floor, Guangzhou Xianlie Middle Road 100 Courtyard, the preservation number is GDMCC No: 67293, which can be directional enrichment of curcumin, solve the defects of limited biological transformation pathway, insufficient biological transformation capacity and low conversion efficiency of traditional strains.

The invention discloses a completely biodegradable layered composite material and a product thereof. The layered composite material has at least three layers of structures, and comprises an outer layer, a barrier layer and an inner layer from outside to inside. The composition of both the outer layer and the inner layer comprises a polyolefin and a bioconversion additive, the additive containing a non-enzymatic catalyst. The barrier layer is selected from a high barrier polymer layer and may include an additive or biodegradable polyester to participate in degradation. By designing that the concentration of the biotransformation additive in the outer layer is higher than that of the biotransformation additive in the inner layer and combining protection and collaborative design of the barrier layer, the layered composite material is stable in performance in the use period of a product, and after being discarded and exposed to the natural environment, the layered composite material can be subjected to ordered biotransformation degradation from the outer layer and finally is completely converted into environment-harmless substances; and micro-plastics are not generated. The layered composite material is perfectly compatible with an existing processing and recycling system, is suitable for hoses, packaging films and other products, and solves the contradiction between high-performance packaging and complete environmental degradation.

The invention discloses an in-situ resource conversion and food structure generation system for an extreme physical environment, belongs to the technical field of intersection of extreme environmental engineering and food engineering, and aims to solve the problems that food supply depends on external transportation for a long time and the anti-risk capability of a traditional agricultural system is insufficient in the extreme physical environment. The system comprises a resource acquisition module, a resource conversion module, a food structure generation module and a system operation control module. The system generates food base components by obtaining environmental in-situ resources, using biotransformation links, non-biotransformation links, or a combination thereof, and using physical conditions in an extreme environment as processing boundary conditions to perform structured, conformal, or ingestible treatment on the food base components or biological products, such that the food base components or biological products are obtained. And a structured food for human ingestion is generated. The system operation control module dynamically adjusts operation parameters according to a resource state, an energy state or a module operation state, triggers a degradation operation strategy under an abnormal or limited working condition, and preferentially maintains continuous supply of basic food. According to the invention, long-term operation can be realized without external continuous material supply, and the system is suitable for non-earth or non-earth-like extreme physical environments.

The application provides a method for synthesizing cyanidin by whole cell transformation. The method comprises the following steps: inducing culture of a recombinant strain S2; and inoculating the bacteria obtained by the induced culture into a culture medium containing a substrate 1-(4-hydroxyphenol)-ethanol to perform whole cell transformation, so as to synthesize the cyanidin. The application provides a biological transformation method for synthesizing cyanidin instead of a chemical synthesis method and a natural extraction method. The recombinant Escherichia coli is used to transform 1-(4-hydroxyphenol)-ethanol into cyanidin, and the method has the advantages of mild reaction condition, low production cost and environmental protection.

The invention belongs to the technical field of feeds, and discloses a peanut cake meal fermented detoxified marine aquatic feed and a preparation method thereof. The feed is mainly prepared from peanut meal which is subjected to synergistic fermentation treatment by compound probiotics (bacillus subtilis and saccharomyces cerevisiae) and compound enzymes (phytase and alkaline protease), and is supplemented with an antioxidant and a mildew preventive; according to the preparation method, two-stage temperature-control solid-state fermentation is adopted, pH is dynamically regulated and controlled through flora metabolism in a micro-aerobic environment, and synchronous efficient degradation of phytic acid and lectin and directional hydrolysis of protein are achieved. A biotransformation system which is complete in structure, synergetic in function and feasible in engineering is constructed, efficient removal of phytic acid and agglutinin in the peanut cakes and directional hydrolysis activation of protein are synchronously achieved under a single process path for the first time, and the technical bottleneck of application of the peanut cakes in high-end marine aquatic feed is fundamentally broken through; and a new technical normal form is provided for high-value utilization of plant protein resources.

The present invention relates to methods for biological wastewater treatment for Se control in Se-laden wastewater. The Se contaminants in the wastewater include the Se oxyanions selenate (SeO42−) and selenite (HSeO3−), which are biochemically reduced and transformed to elemental selenium (Se0) by microorganisms through anaerobic biological reduction. The resulting Se0 is entrained in the biomass, which is further processed to enable the efficient recovery of concentrated Se0.

The invention belongs to the technical field of fungus culture and cultivation, and particularly relates to a wild Pleurotus ostreatus strain, a domestication and cultivation method and application of the wild Pleurotus ostreatus strain. The wild pleurotus ostreatus ZGCL25-8-11-11 is a high-temperature-resistant strain, the fruiting temperature ranges from 20 DEG C to 28 DEG C, sporocarp growing under the high-temperature condition is black gray, the sporocarp is subordinate, the single-bag yield of one flush of pleurotus ostreatus is at least 452 g, and the biological conversion rate is at least 85%. And the pleurotus ostreatus strain is dense in hypha and high in growth speed, so that the strain culture time in the cultivation material is shortened, the strain culture cost is reduced, and the defects that the existing pleurotus ostreatus strain is poor in high-temperature adaptability, low in growth speed and low in crude protein content are overcome.

The present invention relates to bio-transformed milk products, for example evaporated milk or similar products, that have improved properties and / or characteristics relative to equivalent non-bio-transformed milk products; or have comparable sensory characteristics to non-bio- transformed milk products that comprise greater quantities of milk solids. The invention also encompasses methods for making said bio-transformed milk products.

The present application belongs to the technical field of sewage treatment, and particularly relates to a preparation process of an iron salt-chitosan composite modified microbial high-adaptation type biochar. The present application combines an iron salt-chitosan composite modified biochar carrier with arsenic and antimony resistance functional bacteria, and the composite modified biochar not only retains excellent pore structure and specific surface area, but also forms a micro-ecosystem of nutrient release and micro-environment buffering through the synergistic effect between materials. The immobilized functional bacteria further degrade or transform As and Sb forms through biological transformation, realize the synergistic effect of multiple mechanisms, and greatly improve the removal efficiency of arsenic and antimony composite pollution. Moreover, the raw material biochar of the present application is widely available and low in cost, chitosan and iron salt are both environmentally compatible materials, the overall preparation process is green and environmentally friendly, and the prepared biochar can be applied to the treatment of high-concentration and complex-component arsenic and antimony contaminated wastewater, and has good application value.

The invention provides an oil-soluble plant compound and a preparation method thereof, and belongs to the field of medical, dental or cosmetic preparations. The composition consists of chrysanthellum indicum, ligusticum wallichii, frankincense, exocarpium citri grandis essential oil and a soybean-radix puerariae co-fermentation product. The technical core is as follows: soybean and radix puerariae are subjected to synergistic liquid fermentation by using aspergillus oryzae, lysophosphatide, soybean polypeptide and puerarin aglycone are generated in situ through biotransformation, and an endogenous self-emulsifying carrier is constructed. According to the process, the transdermal penetration promoting capacity of active components is remarkably enhanced by utilizing the regulating effect of ligusticum wallichii oil on cuticle lipid arrangement and combining a micro-micelle structure formed by a co-fermentation system. Nanoscale self-solubilization of four essential oil matrixes in a fermentation substrate is achieved, the skin barrier repair and inflammation inhibition effects are enhanced through multi-target cooperation, and the bioavailability, penetration depth and safety of active ingredients are greatly improved.

A carbon dioxide-neutral biodigester plant includes a biodigester for carbon dioxide-neutral, single-stage or multi-stage production of biogas by fermentation of biomass in a fermentation liquid agitated in presence of elemental hydrogen, hydrogenotrophic and methanogenic archaea, and activated carbon masses, a source of ammonia connected to a first ammonia line, an ammonia cracker to produce hydrogen or a hydrogen-nitrogen mixture by catalytic cracking of ammonia, a first hydrogen line for feeding the produced hydrogen or the hydrogen-nitrogen mixture directly into the biodigester, and a treating device for treating activated coal masses with hydrogen at higher pressure. The treating device includes a pressure line and a treating spray lance. Hydrogen-containing activated coal masses configured as spray cones are injected into the fermentation liquid of the biodigester.

The application belongs to the technical field of biology and particularly relates to a beta-glucosidase, a coding gene, a recombinant carrier, engineered bacteria and application. The beta-glucosidase is derived from Thermomyces lanuginosus DSM 10635; the amino acid sequence of the beta-glucosidase is shown in SEQ ID NO: 2. The application further provides a beta-glucosidase derived from T. lanuginosus DSM 10635 and a coding gene thereof; the beta-glucosidase coding gene can be connected with an expression carrier, transformed into a Pichia pastoris strain, and a recombinant Pichia pastoris expressing the beta-glucosidase is constructed; and the beta-glucosidase with good thermal stability expressed by the recombinant Pichia pastoris is used as a catalyst to prepare ginsenoside CK through biological transformation.

The invention relates to a plant endophyte as well as a preparation process and application of a fermentation product of the plant endophyte, and in particular, the plant endophyte is dendrobium officinale endophyte candida Gandida sp. IGLS2, and the preservation number is CCTCC (China Center For Type Culture Collection) M20242143. The endophyte can be fermented to obtain dendrobium officinale fermentation liquor without an additional carbon source, dendrobium polysaccharides can be effectively degraded, biotransformation is achieved, in addition, compared with an unfermented dendrobium officinale extracting solution, the capacity of inhibiting ROS and IL-6 is effectively improved, the cell migration rate is remarkably improved, and the cell activity is not affected under high concentration.

The application provides a method for analyzing migration and transformation rules of nitrogen and phosphorus in sewage resource utilization, and relates to the technical field of sewage resource utilization. The method comprises the following steps: synchronously sampling and measuring nitrogen and phosphorus concentrations from source chambers, medium chambers and receiving chambers at different depths, and calculating apparent permeation flux and adsorption retardation coefficient; taking the difference between apparent permeation fluxes of nitrate nitrogen in the presence and absence of an inhibitor unit as an in-situ nitrification rate representation value, taking the difference between apparent permeation fluxes of ammonium nitrogen as an in-situ mineralization rate representation value, and taking the ratio between the sum of the two and the total nitrogen apparent permeation flux as a biological transformation contribution index; finally, based on the indexes at different depths, a vertical distribution map is drawn, a biological transformation dominant area, an adsorption and fixation dominant area and a physical permeation dominant area are demarcated, and a partition control scheme is output, so as to realize quantitative separation of physical permeation, adsorption and fixation and microbial transformation contribution, and establish a partition precise control mechanism based on the dominant action type.

The application belongs to the technical field of microbial medicine, and specifically discloses a bacillus velezensis TK1926 and application thereof in repair of gastric mucosa. Through systematic evaluation of multiple models and multiple indexes, it is confirmed that TK1926 fermentation treatment can comprehensively enhance the gastric mucosa protection and treatment effect of radix codonopsis. Compared with unfermented radix codonopsis, fermented radix codonopsis shows improved performance in anti-inflammatory, antioxidant, barrier repair and pathogen inhibition, which is the result of multi-dimensional synergistic effect such as anti-inflammatory-antioxidant-barrier repair-regulation of gastric environment. The fermentation process improves the bioavailability and efficacy of the original active ingredients of radix codonopsis through biological transformation, and may produce new beneficial metabolites, thereby exerting a synergistic enhancement effect. The fermentation product provided by the application can be used for preparing medicines to repair gastric mucosa damage, and provides a new method for intervention of gastric mucosa damage related diseases, and has a good application prospect.