Flexible mycelium composite material as well as preparation process and application thereof

Abstract

The invention belongs to the field of bioengineering, and particularly relates to a flexible mycelium composite material as well as a preparation process and application thereof. The flexible mycelium composite material disclosed by the invention is prepared by inoculating an edible fungus seed solution into a lignocellulose matrix, mixing, filling a mold and integrally fermenting, and the preparation process is simple. According to the method, rhamnolipid is added into an edible fungus seed solution culture medium, so that the enzyme activity of cellulase in the seed solution is effectively improved, the degradation and utilization of cellulose and hemicellulose in a lignocellulose matrix are further improved, the planting capacity of mycelia on the surface of lignocellulose is improved, the biomass of the mycelia is improved, and the yield of the mycelia is increased. Finally, the toughness of the flexible mycelium composite material is improved, so that the bending property of the flexible mycelium composite material is improved. Meanwhile, the flexible mycelium composite material prepared by the preparation method disclosed by the invention is biologically converted by utilizing agricultural and forestry wastes, the material utilization rate can reach 100%, and the flexible mycelium composite material is free from environmental pollution and has a wide industrial application prospect.

Description

Technical Field

[0001] The present invention belongs to the field of bioengineering, and in particular relates to a flexible mycelium composite material and a preparation process and application thereof. Background Art

[0002] With the increasing emphasis on carbon reduction in the packaging industry, green transformation in the packaging industry is becoming increasingly important. Currently, mainstream cushioning packaging materials are mainly pulp molding, EPS, PET, etc. Although pulp molding can effectively sequester carbon, it relies heavily on imported pulp and still cannot meet the current demand for green materials and production processes. Compared with the carbon emissions of materials such as EPS, the production of 1m 3 Mycelium composites can reduce carbon dioxide emissions by approximately 263 kg, making them highly effective carbon sequestration materials. Performance analysis shows that mycelium composites can achieve a compression resistance of 0.07 MPa, meeting the requirements of the national standard "GB / T 8813-2020." Therefore, in terms of carbon sequestration and emission reduction capabilities, mycelium composites are one of the few excellent alternatives, potentially solving packaging challenges and achieving green and sustainable upgrades in the packaging materials industry.

[0003] Mycelium is cultivated in a culture medium rich in cellulose, hemicellulose, lignin, and carbon and nitrogen sources to form a three-dimensional interwoven mycelial network, which is then cultivated into a bioreinforcement material containing mycelial fibers. This process is highly dependent on the strain's ability to utilize and convert lignocellulose, and the abundant extracellular enzymes are key to the strain's ability to colonize the substrate surface, ultimately forming a mycelium with wrapping properties. Currently, mycelium composite materials prepared using existing technologies have low biomass, resulting in poor bending properties and average toughness, making them unsuitable for use as cushioning packaging materials.

[0004] Therefore, it is of great significance to develop a flexible mycelium composite material with high biomass, good bending performance and good toughness. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a flexible mycelium composite material for the mycelium composite material prepared in the prior art, which has a low biomass, resulting in poor bending performance and general toughness of the material, and cannot be used well as a cushioning packaging material.

[0006] Another technical problem to be solved by the present invention is to provide a preparation process of a flexible mycelium composite material.

[0007] The final technical problem to be solved by the present invention is to provide an application of the flexible mycelium composite material in the preparation of cushioning packaging materials.

[0008] In order to solve the above technical problems, the present invention discloses a flexible mycelium composite material, which is prepared by inoculating edible fungus seed liquid into a lignocellulose matrix, mixing, filling a mold, and fermenting it in one piece.

[0009] The edible fungus seed liquid is a seed liquid containing cellulase activity, which is obtained by culturing in a seed culture medium containing rhamnolipid.

[0010] Specifically, the rhamnolipid is an inducer of cellulase.

[0011] Wherein, the lignocellulose is straw and / or sawdust.

[0012] In some embodiments of the present invention, the lignocellulose is a mixture of straw and sawdust.

[0013] The edible fungi include any one of Ganoderma lucidum, Pleurotus ostreatus, Enoki mushroom, Pleurotus ostreatus and Volvariella volvacea.

[0014] The preferred edible fungus is Ganoderma lucidum; in some embodiments of the present invention, the edible fungus is red Ganoderma lucidum.

[0015] The concentration of the rhamnolipid in the seed culture medium is 0.01 to 0.3 g / L; the preferred concentration is 0.05 to 0.3 g / L; the further preferred concentration is 0.15 to 0.3 g / L; the more preferred concentration is 0.25 to 0.3 g / L; and the most preferred concentration is 0.25 g / L.

[0016] Among them, the flexible mycelium composite material has a biomass of 15 to 30 wt% and a bending strength of 0.05 to 0.4 MPa; the preferred biomass is 17 to 30 wt% and the bending strength is 0.08 to 0.4 MPa; the further preferred biomass is 20 to 30 wt% and the bending strength is 0.13 to 0.4 MPa; the more preferred biomass is 27 to 30 wt% and the bending strength is 0.37 to 0.4 MPa; the most preferred biomass is 27 wt% and the bending strength is 0.4 MPa.

[0017] Specifically, the biomass refers to the weight proportion of mycelium cells in the entire flexible mycelium composite material.

[0018] The preparation method of the flexible mycelium composite material is also within the scope of protection of the present invention.

[0019] Specifically, the edible fungus seed liquid is mixed with the lignocellulose matrix, and then the mixture is molded and fermented, and then demolded and dried to obtain the flexible mycelium composite material.

[0020] The edible fungus seed liquid is prepared by inoculating edible fungi into a seed liquid culture medium and fermenting the culture medium.

[0021] Specifically, the edible fungus seed liquid is rich in cellulase activity, and the filter paper enzyme activity in the supernatant is measured to be 1.7 to 5 FPU / mL by a cellulase activity detection method.

[0022] Specifically, the fermentation conditions are: temperature 23-27°C, rotation speed 150-170 rpm, and time 4-7 days; in some embodiments of the present invention, the fermentation conditions are: temperature 26°C, rotation speed 150 rpm, and time 5 days.

[0023] The seed culture medium has the following formula: 10-30 g / L glucose, 1-3 g / L yeast extract powder, 0.01-0.3 g / L rhamnolipid, 0.1-0.3 g / L MgSO4, 0.1-0.3 g / L FeSO4·7H2O, 0.08-0.12 g / L KH2PO4, and 0.08-0.12 g / L VB1.

[0024] In some embodiments of the present invention, the seed culture medium has a formula of: 20 g / L glucose, 3 g / L yeast extract, 0.05-0.3 g / L rhamnolipid, 0.15 g / L MgSO4, 0.15 g / L FeSO4·7H2O, 0.1 g / L KH2PO4, and 0.1 g / L VVB1.

[0025] The lignocellulose matrix comprises the following components in weight percentage: 25-50 wt% straw and 50-75 wt% sawdust.

[0026] In some embodiments of the present invention, the lignocellulosic matrix comprises the following components in weight percentage: 30 wt % straw, 70 wt % sawdust.

[0027] Wherein, the inoculation amount of the edible fungus seed liquid is 10-30% v / v of the total volume of the lignocellulose matrix.

[0028] In some embodiments of the present invention, the inoculation amount of the edible fungus seed liquid is 20% v / v of the total volume of the lignocellulose substrate.

[0029] The mold-filling fermentation is carried out under the following conditions: a temperature of 23 to 27° C. and static culture for 4 to 7 days.

[0030] In some embodiments of the present invention, the mold-filling fermentation is carried out under the following conditions: a temperature of 26° C. and a static culture time of 7 days.

[0031] The conditions for the demoulding and drying are as follows: a drying temperature of 50 to 80° C. and a drying time of 2 to 24 hours.

[0032] In some embodiments of the present invention, the demoulding and drying process is carried out under the following conditions: drying temperature of 60° C. and drying time of 24 hours.

[0033] The application of the above-mentioned flexible mycelium composite material in the preparation of cushioning packaging materials is also within the scope of protection of the present invention.

[0034] Specifically, in some embodiments of the present invention, by preparing a flexible mycelium composite material and testing its biomass and bending strength, it is proved that the flexible mycelium composite material provided by the present invention has high mycelium biomass, good bending strength and toughness, and can be used in cushioning packaging materials.

[0035] Beneficial effects:

[0036] 1. The present invention prepares a flexible mycelium composite material by inoculating edible fungus seed liquid into a lignocellulose matrix, mixing, filling molds, and integrated fermentation. The material has high biomass and good bending strength and toughness. It can be used as a cushioning packaging material and has a good cushioning effect.

[0037] 2. The flexible mycelium composite material prepared by the present invention is obtained by biotransformation of agricultural and forestry wastes, and the material utilization rate can reach 100%. No chemical products such as industrial adhesives are added during the production process, so there is no volatile organic compound (VOC) emission, and it is an environmentally friendly material.

[0038] 3. In the process of preparing mycelium composite materials, the present invention increases the enzymatic activity of cellulase in the seed liquid by adding rhamnolipid to the culture medium of edible fungus seed liquid, further effectively improves the degradation and utilization of cellulose and hemicellulose in the lignocellulose matrix, thereby improving the colonization ability of mycelium on the surface of lignocellulose and increasing the biomass of mycelium, which ultimately feeds back to the improvement of the flexibility of the mycelium composite material and significantly improves the bending performance of the flexible mycelium composite material.

[0039] 4. The preparation process of the mycelium composite material of the present invention is simple and has broad prospects for industrial and industrial application. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more apparent.

[0041] Figure 1 The following are SEM images of the mycelium composite material matrix prepared in the present invention, wherein A is the SEM image of the mycelium composite material of Comparative Example 1, and B is the SEM image of the flexible mycelium composite material of Example 1.

[0042] Figure 2The figures show the mycelium growth process on the surface of the mycelium composite materials of Comparative Example 1 and Example 1. The first three figures show the mycelium growth process of the mycelium composite material of Comparative Example 1 on days 2, 4, and 6, and the second three figures show the mycelium growth process of the flexible mycelium composite material of Example 1 on days 2, 4, and 6. DETAILED DESCRIPTION

[0043] The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.

[0044] In the following examples, the Ganoderma lucidum strain (Red Ganoderma lucidum) was purchased from Tianda Edible Fungi Research Institute.

[0045] In the following examples, the cellulase activity detection method is referred to the document "Study on strategies to enhance the ability of Penicillium oxalicum cellulase system to hydrolyze lignocellulose".

[0046] In the following examples, in the performance test, the biomass detection method refers to the biomass detection method of the solid-state fermentation product in the document "Study on the Physicochemical Characteristics of Solid-State Fermentation Medium and New Solid-State Fermentation Process".

[0047] In the following embodiments, in the performance test, the bending strength is tested using a mechanical performance testing device, a universal testing machine, and the testing standard refers to the national standard "GBT 8812.2-2007".

[0048] Example 1: Preparation of flexible mycelium composite material

[0049] 1. Preparation of Ganoderma lucidum seed liquid

[0050] The purchased Ganoderma lucidum strains were inoculated into the seed liquid culture medium and cultured at 26° C. and 150 rpm for 5 days to obtain Ganoderma lucidum seed liquid.

[0051] The formula of the seed liquid culture medium is as follows: 20g / L glucose (basic carbon source), 3g / L yeast extract powder (basic nitrogen source), 0.25g / L rhamnolipid (inducer), 0.15g / L MgSO4, 0.15g / L FeSO4·7H2O, 0.1g / L KH2PO4, and 0.1g / L VB1.

[0052] After adding 0.25 g / L rhamnolipid to the seed liquid culture medium for cellulase induction, according to the cellulase activity detection method, the Ganoderma lucidum seed liquid was rich in cellulase activity, and the filter paper enzyme activity in the supernatant was 5 FPU / mL.

[0053] 2. Preparation of flexible mycelium composite materials

[0054] In a clean bench, Ganoderma lucidum seed liquid was inoculated into a lignocellulosic matrix (the lignocellulosic matrix included the following weight percentages: 30wt% corn straw and 70wt% sawdust) sterilized at 115°C for 60 minutes at a rate of 20% v / v of the total volume of the lignocellulosic matrix. After stirring evenly, the mixture was filled into a mold, sealed, and placed in a shaker at 26°C for 7 days for solid-state fermentation to prepare a mycelium material. After the solid-state fermentation was completed, the mycelium material, which had been shaped and covered with white mycelium, was removed from the mold and placed in a 60°C oven for drying and dehydration for 24 hours to inactivate the bacteria inside the matrix and stop their growth, thereby obtaining a flexible mycelium composite material.

[0055] The prepared flexible mycelium composite material was tested for performance using a universal testing machine, and the test results were as follows: biomass 27 wt%, bending strength: 0.4 MPa.

[0056] Example 2: Preparation of flexible mycelium composite material

[0057] 1. Preparation of Ganoderma lucidum seed liquid

[0058] The purchased Ganoderma lucidum strains were inoculated into the seed liquid culture medium and cultured at 26° C. and 150 rpm for 5 days to obtain Ganoderma lucidum seed liquid.

[0059] The formula of the seed liquid culture medium is as follows: 20g / L glucose (basic carbon source), 3g / L yeast extract powder (basic nitrogen source), 0.05g / L rhamnolipid (inducer), 0.15g / L MgSO4, 0.15g / L FeSO4·7H2O, 0.1g / L KH2PO4, and 0.1g / L VB1.

[0060] After adding 0.05 g / L rhamnolipid to the seed liquid culture medium for cellulase induction, according to the cellulase activity detection method, the Ganoderma lucidum seed liquid was rich in cellulase activity, and the filter paper enzyme activity in the supernatant was 1.7 FPU / mL.

[0061] 2. Preparation of flexible mycelium composite materials

[0062] In a clean bench, Ganoderma lucidum seed liquid was inoculated into a lignocellulosic matrix (the lignocellulosic matrix included the following weight percentages: 30wt% corn straw and 70wt% sawdust) sterilized at 115°C for 60 minutes at a rate of 20% v / v of the total volume of the lignocellulosic matrix. After stirring evenly, the mixture was filled into a mold, sealed, and placed in a shaker at 26°C for 7 days for solid-state fermentation to prepare a mycelium material. After the solid-state fermentation was completed, the mycelium material, which had been shaped and covered with white mycelium, was removed from the mold and placed in a 60°C oven for drying and dehydration for 24 hours to inactivate the bacteria inside the matrix and stop their growth, thereby obtaining a flexible mycelium composite material.

[0063] The prepared flexible mycelium composite material was tested for performance using a universal testing machine, and the test results were as follows: biomass: 17 wt%; flexural strength: 0.08 MPa.

[0064] Example 3: Preparation of flexible mycelium composite material

[0065] 1. Preparation of Ganoderma lucidum seed liquid

[0066] The purchased Ganoderma lucidum strains were inoculated into the seed liquid culture medium and cultured at 26° C. and 150 rpm for 5 days to obtain Ganoderma lucidum seed liquid.

[0067] The formula of the seed liquid culture medium is as follows: 20g / L glucose (basic carbon source), 3g / L yeast extract powder (basic nitrogen source), 0.15g / L rhamnolipid (inducer), 0.15g / L MgSO4, 0.15g / L FeSO4·7H2O, 0.1g / L KH2PO4, and 0.1g / L VB1.

[0068] After adding 0.15 g / L rhamnolipid to the seed liquid culture medium for cellulase induction, according to the cellulase activity detection method, the Ganoderma lucidum seed liquid was rich in cellulase activity, and the filter paper enzyme activity in the supernatant was 2.3 FPU / mL.

[0069] 2. Preparation of flexible mycelium composite materials

[0070] In a clean bench, Ganoderma lucidum seed liquid was inoculated into a lignocellulosic matrix (the lignocellulosic matrix included the following weight percentages: 30wt% corn straw and 70wt% sawdust) sterilized at 115°C for 60 minutes at a rate of 20% v / v of the total volume of the lignocellulosic matrix. After stirring evenly, the mixture was filled into a mold, sealed, and placed in a shaker at 26°C for 7 days for solid-state fermentation to prepare a mycelium material. After the solid-state fermentation was completed, the mycelium material, which had been shaped and covered with white mycelium, was removed from the mold and placed in a 60°C oven for drying and dehydration for 24 hours to inactivate the bacteria inside the matrix and stop their growth, thereby obtaining a flexible mycelium composite material.

[0071] The prepared flexible mycelium composite material was tested for performance using a universal testing machine, and the test results were as follows: biomass: 20 wt%; flexural strength: 0.13 MPa.

[0072] Example 4: Preparation of flexible mycelium composite material

[0073] 1. Preparation of Ganoderma lucidum seed liquid

[0074] The purchased Ganoderma lucidum strains were inoculated into the seed liquid culture medium and cultured at 26° C. and 150 rpm for 5 days to obtain Ganoderma lucidum seed liquid.

[0075] The formula of the seed liquid culture medium is as follows: 20g / L glucose (basic carbon source), 3g / L yeast extract powder (basic nitrogen source), 0.2g / L rhamnolipid (inducer), 0.15g / L MgSO4, 0.15g / L FeSO4·7H2O, 0.1g / L KH2PO4, and 0.1g / L VB1.

[0076] After adding 0.2 g / L rhamnolipid to the seed liquid culture medium for cellulase induction, according to the cellulase activity detection method, the Ganoderma lucidum seed liquid was rich in cellulase activity, and the filter paper enzyme activity in the supernatant was 3.2 FPU / mL.

[0077] 2. Preparation of flexible mycelium composite materials

[0078] In a clean bench, Ganoderma lucidum seed liquid was inoculated into a lignocellulosic matrix (the lignocellulosic matrix included the following weight percentages: 30wt% corn straw and 70wt% sawdust) sterilized at 115°C for 60 minutes at a rate of 20% v / v of the total volume of the lignocellulosic matrix. After stirring evenly, the mixture was filled into a mold, sealed, and placed in a shaker at 26°C for 7 days for solid-state fermentation to prepare a mycelium material. After the solid-state fermentation was completed, the mycelium material, which had been shaped and covered with white mycelium, was removed from the mold and placed in a 60°C oven for drying and dehydration for 24 hours to inactivate the bacteria inside the matrix and stop their growth, thereby obtaining a flexible mycelium composite material.

[0079] The prepared flexible mycelium composite material was tested for performance using a universal testing machine, and the test results were as follows: biomass: 22 wt%; flexural strength: 0.21 MPa.

[0080] Example 5: Preparation of flexible mycelium composite material

[0081] 1. Preparation of Ganoderma lucidum seed liquid

[0082] The purchased Ganoderma lucidum strains were inoculated into the seed liquid culture medium and cultured at 26° C. and 150 rpm for 5 days to obtain Ganoderma lucidum seed liquid.

[0083] The formula of the seed liquid culture medium is as follows: 20g / L glucose (basic carbon source), 3g / L yeast extract powder (basic nitrogen source), 0.3g / L rhamnolipid (inducer), 0.15g / L MgSO4, 0.15g / L FeSO4·7H2O, 0.1g / L KH2PO4, and 0.1g / L VB1.

[0084] After adding 0.3 g / L rhamnolipid to the seed liquid culture medium for cellulase induction, according to the cellulase activity detection method, the Ganoderma lucidum seed liquid was rich in cellulase activity, and the filter paper enzyme activity in the supernatant was 5.1 FPU / mL.

[0085] 2. Preparation of flexible mycelium composite materials

[0086] In a clean bench, Ganoderma lucidum seed liquid was inoculated into a lignocellulosic matrix (the lignocellulosic matrix included the following weight percentages: 30wt% corn straw and 70wt% sawdust) sterilized at 115°C for 60 minutes at a rate of 20% v / v of the total volume of the lignocellulosic matrix. After stirring evenly, the mixture was filled into a mold, sealed, and placed in a shaker at 26°C for 7 days for solid-state fermentation to prepare a mycelium material. After the solid-state fermentation was completed, the mycelium material, which had been shaped and covered with white mycelium, was removed from the mold and placed in a 60°C oven for drying and dehydration for 24 hours to inactivate the bacteria inside the matrix and stop their growth, thereby obtaining a flexible mycelium composite material.

[0087] The prepared flexible mycelium composite material was tested for performance using a universal testing machine, and the test results were as follows: biomass: 30 wt%; flexural strength: 0.37 MPa.

[0088] Combining the rhamnolipid addition amounts and the results of filter paper enzyme activity in the supernatant in Examples 1 to 5, it can be seen that when the rhamnolipid addition amount reaches 0.25 g / L, the filter paper enzyme activity in the supernatant no longer increases with further increase in rhamnolipid addition, and the filter paper enzyme activity in the supernatant remains relatively stable, indicating that when the rhamnolipid addition amount reaches 0.25 g / L, the maximum plateau phase of induction is reached. Furthermore, the corresponding biomass results and bending strength also tend to be stable.

[0089] Comparative Example 1: Effect of not adding rhamnolipid on cellulase activity and material properties

[0090] 1. Preparation of Ganoderma lucidum seed liquid

[0091] The purchased Ganoderma lucidum strains were inoculated into the seed liquid culture medium and cultured at 26° C. and 150 rpm for 5 days to obtain Ganoderma lucidum seed liquid.

[0092] The formula of the seed liquid culture medium is as follows: 20g / L glucose (basic carbon source), 3g / L yeast extract powder (basic nitrogen source), 0.15g / L MgSO4, 0.15g / L FeSO4·7H2O, 0.1g / L KH2PO4, and 0.1g / L VB1.

[0093] According to the cellulase activity detection method, the filter paper enzyme activity in the supernatant of the Ganoderma lucidum seed liquid prepared without adding rhamnolipid is 1 FPU / mL.

[0094] 2. Preparation of mycelium composite materials

[0095] In a clean bench, Ganoderma lucidum seed liquid was inoculated into a lignocellulosic matrix (the lignocellulosic matrix included the following weight percentages: 30wt% corn straw and 70wt% sawdust) sterilized at 115°C for 60 minutes at a rate of 20v / v% of the total volume of the lignocellulosic matrix. After stirring evenly, the mixture was filled into a mold, sealed, and placed in a shaker at 26°C for 7 days for solid-state fermentation to prepare a mycelium material. After the solid-state fermentation was completed, the mycelium material, which had been formed and covered with white mycelium on the surface, was removed from the mold and placed in a 60°C oven for drying and dehydration for 24 hours to inactivate the bacteria inside the matrix and stop their growth, thereby obtaining a mycelium composite material.

[0096] The prepared mycelium composite material was tested for performance using a universal testing machine, and the test results were as follows: biomass: 15 wt%; flexural strength: 0.05 MPa.

[0097] The microscopic morphology of the matrix of the mycelium composite material prepared in Comparative Example 1 and the flexible mycelium composite material prepared in Example 1 was analyzed using a scanning electron microscope. Figure 1 A in the figure is the microscopic morphology of the mycelium composite material of comparative example 1. Figure 1 B in the figure is the microscopic morphology of the flexible mycelium composite material of Example 1. It can be seen from the figure that by adding rhamnolipid, the mycelium colonized on the surface of the matrix inside the mycelium composite material is significantly increased, which ultimately leads to an increase in the bending strength of the mycelium composite material. Figure 2 This is the growth process of mycelium on the surface of the mycelium composite material of Comparative Example 1 and Example 1. It can be clearly seen that the mycelium growth rate and density on the surface of Example 1 are significantly better than those of Comparative Example 1.

[0098] The present invention provides a flexible mycelium composite material, its preparation process, and its application. Numerous methods and approaches exist for implementing this technical solution. The foregoing merely represents a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the present invention. Components not specified in this embodiment may be implemented using existing technologies.

Claims

1. A flexible mycelium composite material, characterized in that: The flexible mycelium composite material is prepared by inoculating edible fungus seed liquid into a lignocellulose matrix, mixing, filling a mold, and fermenting in one piece; Wherein, the edible fungus seed liquid is a seed liquid containing cellulase activity, which is obtained by culturing using a seed culture medium containing rhamnolipid; The flexible mycelium composite material has a biomass of 17 to 30 wt% and a bending strength of 0.08 to 0.4 MPa.

2. The flexible mycelium composite material according to claim 1, characterized in that: The edible fungi include any one of Ganoderma lucidum, Pleurotus ostreatus, Enoki mushroom, Pleurotus ostreatus and Volvariella volvacea; and the lignocellulose matrix is ​​straw and / or sawdust.

3. The flexible mycelium composite material according to claim 1, characterized in that: The concentration of the rhamnolipid in the seed culture medium is 0.01-0.3 g / L.

4. The method for preparing the flexible mycelium composite material according to any one of claims 1 to 3, characterized in that: The flexible mycelium composite material is obtained by mixing edible fungus seed liquid with a wood cellulose matrix, performing mold filling and fermentation, and then demolding and drying.

5. The preparation method according to claim 4, characterized in that The edible fungus seed liquid is prepared by inoculating edible fungi into a seed liquid culture medium and fermenting the culture medium; the fermentation conditions are: temperature 23-27° C., rotation speed 150-170 rpm, and time 4-7 days.

6. The preparation method according to claim 5, characterized in that The seed culture medium has the following formula: 10-30 g / L glucose, 1-3 g / L yeast extract powder, 0.01-0.3 g / L rhamnolipid, 0.1-0.3 g / L MgSO4, 0.1-0.3 g / L FeSO4·7H2O, 0.08-0.12 g / L KH2PO4, and 0.08-0.12 g / L VB1.

7. The preparation method according to claim 4, characterized in that The lignocellulose matrix comprises the following components in weight percentage: 25-50 wt% straw and 50-75 wt% sawdust.

8. The preparation method according to claim 4, characterized in that The inoculation amount of the edible fungus seed liquid is 10-30% v / v of the total volume of the lignocellulose.

9. The preparation method according to claim 4, characterized in that The conditions for the mold filling fermentation are: a temperature of 23-27° C. and a static culture of 4-7 days; the conditions for the mold removal and drying are: a drying temperature of 50-80° C. and a time of 2-24 hours.

10. Use of the flexible mycelium composite material according to any one of claims 1 to 3 in the preparation of cushioning packaging materials.

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