Wild pleurotus ostreatus strain, domestication and cultivation method and application of wild pleurotus ostreatus strain

Abstract

The invention belongs to the technical field of fungus culture and cultivation, and particularly relates to a wild Pleurotus ostreatus strain, a domestication and cultivation method and application of the wild Pleurotus ostreatus strain. The wild pleurotus ostreatus ZGCL25-8-11-11 is a high-temperature-resistant strain, the fruiting temperature ranges from 20 DEG C to 28 DEG C, sporocarp growing under the high-temperature condition is black gray, the sporocarp is subordinate, the single-bag yield of one flush of pleurotus ostreatus is at least 452 g, and the biological conversion rate is at least 85%. And the pleurotus ostreatus strain is dense in hypha and high in growth speed, so that the strain culture time in the cultivation material is shortened, the strain culture cost is reduced, and the defects that the existing pleurotus ostreatus strain is poor in high-temperature adaptability, low in growth speed and low in crude protein content are overcome.

Description

Technical Field

[0001] This invention belongs to the field of fungal culture and cultivation technology, specifically relating to a wild Pleurotus ostreatus strain, its domestication and cultivation method, and its application. Background Technology

[0002] Rough-skinned side ear ( Pleurotus ostreatus Oyster mushroom, also known as oyster mushroom, is a widely cultivated edible fungus. It is not only rich in nutrients such as protein and amino acids, but also rich in polysaccharides, sterols and other active ingredients. It has a variety of effects such as enhancing immunity and regulating metabolism. At the same time, it can decompose organic matter such as lignocellulose in nature and participate in the material cycle of the ecosystem, thus playing an important ecological role.

[0003] Currently, the resources of *Pleurotus ostreatus* strains are relatively abundant, and the supply of high-quality, high-yield strains in the market is relatively stable. However, there are still some issues that need optimization in terms of germplasm resources. On the one hand, wild *Pleurotus ostreatus* germplasm resources are affected by factors such as habitat destruction and over-collection, threatening the survival environment of some wild populations. On the other hand, most *Pleurotus ostreatus* strains have poor heat tolerance, and cultivation during the hot summer season easily leads to problems such as slow mycelial growth, pale fruiting body color, and reduced yield. Thirdly, the domestication of wild strains is difficult, making it hard to simultaneously achieve high-temperature adaptability, growth rate, and nutritional quality, which also limits the development and utilization of *Pleurotus ostreatus* strain resources.

[0004] Therefore, it is urgent to discover and screen new strains of Pleurotus ostreatus with excellent characteristics, so as to provide new materials for the breeding of superior strains of Pleurotus ostreatus and the protection of germplasm resources, and to cultivate new strains of Pleurotus ostreatus that have strong high temperature tolerance, fast growth rate and high content of nutrients (especially crude protein). Summary of the Invention

[0005] The purpose of this invention is to provide a wild Pleurotus ostreatus strain, a domestication and cultivation method, and its application. The wild Pleurotus ostreatus strain is heat-resistant and grows rapidly. After domestication, the fruiting bodies obtained have a high nutrient content.

[0006] This invention provides a wild Pleurotus ostreatus plant ZGCL25-8-11-11, with accession number CGMCC No.42099.

[0007] The present invention also provides a pure mycelial strain of wild Pleurotus ostreatus ZGCL25-8-11-11 as described in the above technical solution, which is a strain obtained by transferring the germinating mycelium of wild Pleurotus ostreatus ZGCL25-8-11-11 as described in the above technical solution to a culture medium.

[0008] The present invention also provides the application of the wild Pleurotus ostreatus ZGCL25-8-11-11 or the pure mycelial strain described in the above-mentioned technical solutions in the cultivation of at least one of the Pleurotus ostreatus mother strain, the original strain of Pleurotus ostreatus, and the cultivated strain of Pleurotus ostreatus.

[0009] The present invention also provides a mother culture of Pleurotus ostreatus, which is obtained by inoculating the pure mycelial strain described in the above technical solution into a mother culture medium and then incubating it in the dark for the first time; the mother culture medium comprises the following components at the following concentrations: potato 190~210 g / L, glucose 20~30 g / L, magnesium sulfate 1.0~2.0 g / L, potassium dihydrogen phosphate 2.5~3.5 g / L, and natural pH.

[0010] The present invention also provides a primary strain of Pleurotus ostreatus, which is obtained by inoculating the Pleurotus ostreatus mother strain described in the above technical solution into a primary strain cultivation material and then incubating it in the dark for a second time; the primary strain cultivation material comprises the following components in weight percentage: sawdust 55%~65%, corn cob 15%~25%, wheat bran 16%~20%, quicklime 0.5%~1.5% and gypsum 0.5%~1.5%.

[0011] The present invention also provides a cultivar of Pleurotus ostreatus, which is obtained by inoculating the original Pleurotus ostreatus cultivar described in the above technical solution into a well-rotted cultivation substrate for mycorrhizal cultivation; the cultivation substrate comprises the following components in weight percentage: corn cob 75%~85%, wheat bran 10~20%, rice bran 2.5%~3.5%, quicklime 0.5%~1.5% and gypsum 0.5%~1%.

[0012] Preferably, the bacterial culture is carried out in the dark, the culture temperature is 20~25℃, the humidity is 40%~50%, and the culture time is 25~30 days.

[0013] This invention also provides a method for the domestication and cultivation of wild Pleurotus ostreatus ZGCL25-8-11-11, comprising the following steps: The pure mycelial strain described in the above technical solution is inoculated into the mother culture medium and subjected to a first dark culture to obtain the mother culture; the mother culture medium includes the following components at the following concentrations: potato 190~210 g / L, glucose 20~30 g / L, magnesium sulfate 1.0~2.0 g / L, potassium dihydrogen phosphate 2.5~3.5 g / L, and natural pH.

[0014] The mother seed is inoculated into the original seed culture medium for a second dark culture to obtain the original seed of Pleurotus ostreatus; the original seed culture medium includes the following components by weight percentage: sawdust 55%~65%, corn cob 15%~25%, wheat bran 16%~20%, quicklime 0.5%~1.5% and gypsum 0.5%~1.5%.

[0015] The original cultivar of Pleurotus ostreatus was inoculated into well-rotted cultivation substrate to obtain the cultivar of Pleurotus ostreatus. The cultivation substrate comprises the following components by weight percentage: 75%~85% corn cob, 10~20% wheat bran, 2.5%~3.5% rice bran, 0.5%~1.5% quicklime, and 0.5%~1% gypsum.

[0016] The rough-skinned pleurotus ostreatus cultivar was subjected to fruiting culture to obtain rough-skinned pleurotus ostreatus fruiting bodies.

[0017] Preferably, the temperature of the first dark incubation is 25~27℃, and the time of the first dark incubation is 6~9 days; The temperature for the second dark culture was 20-25°C, and the duration of the second dark culture was 6-9 days.

[0018] Preferably, the bacterial culture is carried out in the dark, the culture temperature is 20~25℃, the humidity is 40%~50%, and the culture time is 25~30 days. The temperature for fruiting cultivation is 25~32℃, the relative humidity of the air during fruiting cultivation is 85%~90%, the light intensity during fruiting cultivation is 400~800 lux, and the fruiting cultivation time is 13~18 days.

[0019] Beneficial effects: This invention provides a wild *Pleurotus ostreatus* strain ZGCL25-8-11-11, with preservation number CGMCC No. 42099. Based on this, the invention also provides a pure mycelial strain of wild *Pleurotus ostreatus* ZGCL25-8-11-11, as well as mother culture, primary culture, and cultivar obtained through cultivation; simultaneously, it provides a method for the domestication and cultivation of wild *Pleurotus ostreatus* ZGCL25-8-11-11. The wild *Pleurotus ostreatus* ZGCL25-8-11-11 described in this invention is a heat-resistant strain, with a fruiting temperature of 20-28℃. Under these high-temperature conditions, the fruiting bodies are blackish-gray, and the fruiting bodies grow in clusters. The yield per flush is at least 452 g, and the bioconversion rate is at least 85%. Furthermore, this Pleurotus ostreatus strain has dense mycelium and a fast growth rate, which shortens the cultivation time in the substrate, reduces the cultivation cost, and overcomes the shortcomings of existing Pleurotus ostreatus strains, such as poor high-temperature adaptability, slow growth rate, and low crude protein content.

[0020] Biological Preservation Instructions Wild rough-skinned pleurotus ostreatus ZGCL25-8-11-11, biologically classified as Pleurotus ostreatus, It was deposited on September 11, 2025 at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 42099. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0022] Figure 1 This is a morphological diagram of the wild rough-skinned Pleurotus ostreatus fruiting body in Example 1; Figure 2 This is a diagram illustrating the mycelium of *Pleurotus ostreatus* in Example 1. Figure 3 This is the ZGCL25-8-11-11 phylogenetic tree constructed based on ITS sequences in Example 1; Figure 4 This is a display image of the original species of Pleurotus ostreatus in Example 4; Figure 5 This is a demonstration image of the rough-skinned Pleurotus ostreatus cultivar in Example 4; Figure 6 This is a diagram illustrating the fruiting process of *Pleurotus ostreatus* cultivation in Example 4; Figure 7 This is a diagram illustrating the growth of the fruiting bodies of the rough-skinned lateral ear in Example 4; Figure 8 The results of nutrient composition determination in the fruiting bodies of *Pleurotus ostreatus* and *Pleurotus ostreatus* No. 1 in Example 5 are shown, where different letters indicate significant differences. P <0.05. Detailed Implementation

[0023] This invention provides a wild Pleurotus ostreatus plant ZGCL25-8-11-11, with accession number CGMCC No.42099.

[0024] The wild *Pleurotus ostreatus* ZGCL25-8-11-11 described in this invention was collected from the Zhangguangcailing Laojieji Forest Farm in Shangzhi City, Heilongjiang Province, and named after isolation, purification, and identification. The mycelial morphology of the wild *Pleurotus ostreatus* ZGCL25-8-11-11 is characterized by its pure white, dense mycelium, rapid growth, and vigorous development. The fruiting bodies of the wild *Pleurotus ostreatus* ZGCL25-8-11-11 are blackish-gray; the cap diameter is 4.0-6.0 cm, the stipe is thick, the texture is firm, and it has good marketability. The wild *Pleurotus ostreatus* ZGCL25-8-11-11 exhibits high-temperature adaptability; the mycelium can grow normally in environments ranging from 15-38℃, with the optimal growth temperature being 25-30℃; the fruiting bodies can develop normally in environments ranging from 25-30℃, without deformity under high temperatures, and the fruiting bodies are blackish-gray in color.

[0025] The present invention also provides a pure mycelial strain of wild Pleurotus ostreatus ZGCL25-8-11-11 as described in the above technical solution, which is a strain obtained by transferring the germinating mycelium of wild Pleurotus ostreatus ZGCL25-8-11-11 to a culture medium for purification culture.

[0026] As one embodiment, the method for culturing germinating mycelia of the present invention is as follows: Tissue from the junction of the stipe and cap of a wild-type Pleurotus ostreatus fruiting body (ZGCL25-8-11-11) is inoculated into PDA medium containing antibiotics for germination culture to obtain germinating mycelia. As one embodiment, the antibiotic is a penicillin-streptomycin mixture (100×), with a concentration of 100 U / mL penicillin and 100 μg / mL streptomycin in the PDA medium. As one embodiment, the germination culture temperature is 25°C, and the time is 3 days.

[0027] In one embodiment, the purification culture is conducted at 25°C for 10-12 days. In another embodiment, the purification culture requires repeated transfer of mycelia to the culture medium for cultivation. In yet another embodiment, the culture medium is PDA medium.

[0028] The present invention also provides the application of the wild Pleurotus ostreatus ZGCL25-8-11-11 or the pure mycelial strain described in the above-mentioned technical solutions in the cultivation of at least one of the Pleurotus ostreatus mother strain, the original strain of Pleurotus ostreatus, and the cultivated strain of Pleurotus ostreatus.

[0029] The present invention also provides a mother culture of Pleurotus ostreatus, which is obtained by inoculating the pure mycelial strain described in the above technical solution into a mother culture medium and then incubating it in the dark for the first time; the mother culture medium comprises the following components at the following concentrations: potato 190~210 g / L, glucose 20~30 g / L, magnesium sulfate 1.0~2.0 g / L, potassium dihydrogen phosphate 2.5~3.5 g / L, and natural pH.

[0030] In one embodiment, the mother culture medium comprises the following components at the following concentrations: potato 200 g / L, glucose 25 g / L, magnesium sulfate 1.5 g / L, potassium dihydrogen phosphate 3.0 g / L, and natural pH. In another embodiment, the temperature for the first dark incubation is 25-27°C, or 26-27°C; the duration of the first dark incubation is 6-9 days, or 7-8 days. In an embodiment of the present invention, the inoculation amount of the pure mycelial strain is one inoculation block with a diameter of 1 cm.

[0031] The present invention also provides a primary strain of Pleurotus ostreatus, which is obtained by inoculating the Pleurotus ostreatus mother strain described in the above technical solution into a primary strain cultivation material and then incubating it in the dark for a second time; the primary strain cultivation material comprises the following components in weight percentage: sawdust 55%~65%, corn cob 15%~25%, wheat bran 16%~20%, quicklime 0.5%~1.5% and gypsum 0.5%~1.5%.

[0032] In one embodiment, the original seed culture medium comprises the following components by weight percentage: 60% sawdust, 20% corn cob, 18% wheat bran, 1% quicklime, and 1% gypsum. In another embodiment, the total weight percentage of the original seed culture medium is 100%. In another embodiment, the temperature for the second dark incubation is 20-25°C, or 21-24°C, or 22-23°C; the duration of the second dark incubation is 6-9 days, or 7-8 days. In an embodiment of the present invention, the inoculation quantity of the rough-skinned Pleurotus ostreatus mother seed is 5-8 inoculation blocks with a diameter of 1 cm.

[0033] The present invention also provides a cultivar of Pleurotus ostreatus, which is obtained by inoculating the original Pleurotus ostreatus cultivar described in the above technical solution into a well-rotted cultivation substrate for mycorrhizal cultivation; the cultivation substrate comprises the following components in weight percentage: corn cob 75%~85%, wheat bran 10~20%, rice bran 2.5%~3.5%, quicklime 0.5%~1.5% and gypsum 0.5%~1%.

[0034] In one embodiment, the cultivation medium comprises the following components by weight percentage: 80% corn cob, 15%–16% wheat bran, 3% rice bran, 1% quicklime, and 1% gypsum. In another embodiment, the total weight percentage of the cultivation medium is 100%. In another embodiment, the mycelium is cultured in the dark at a temperature of 20–25°C, or 21–24°C, or 22–23°C; the humidity is 40%–50%; and the incubation period is 25–30 days, or 26–29 days. In another embodiment, the inoculation quantity of the original *Pleurotus ostreatus* spore is 5–8 inoculation blocks with a diameter of 1 cm. In another embodiment, the method for preparing the decomposed cultivation medium is as follows: the cultivation medium is piled up and decomposed at 20–25°C for 12–15 days to obtain the decomposed cultivation medium. In another embodiment, the decomposed cultivation medium is further sterilized before use.

[0035] This invention also provides a method for the domestication and cultivation of wild Pleurotus ostreatus ZGCL25-8-11-11, comprising the following steps: The pure mycelial strain described in the above technical solution is inoculated into the mother culture medium and subjected to a first dark culture to obtain the mother culture; the mother culture medium includes the following components at the following concentrations: potato 190~210 g / L, glucose 20~30 g / L, magnesium sulfate 1.0~2.0 g / L, potassium dihydrogen phosphate 2.5~3.5 g / L, and natural pH.

[0036] The mother seed is inoculated into the original seed culture medium for a second dark culture to obtain the original seed of Pleurotus ostreatus; the original seed culture medium includes the following components by weight percentage: sawdust 55%~65%, corn cob 15%~25%, wheat bran 16%~20%, quicklime 0.5%~1.5% and gypsum 0.5%~1.5%.

[0037] The original cultivar of Pleurotus ostreatus was inoculated into well-rotted cultivation substrate to obtain the cultivar of Pleurotus ostreatus. The cultivation substrate comprises the following components by weight percentage: 75%~85% corn cob, 10~20% wheat bran, 2.5%~3.5% rice bran, 0.5%~1.5% quicklime, and 0.5%~1% gypsum.

[0038] The rough-skinned pleurotus ostreatus cultivar was subjected to fruiting culture to obtain rough-skinned pleurotus ostreatus fruiting bodies.

[0039] The conditions for the first dark culture, the second dark culture, and the culture of bacteria in the domestication and cultivation method of the present invention have been defined in the above technical solution and will not be repeated here.

[0040] In one embodiment, the temperature for fruiting cultivation is 20~28℃, or 24~26℃; the relative humidity of the air for fruiting cultivation is 85%~90%; the light intensity for fruiting cultivation is 400~800 lux, or 500~700 lux; and the fruiting cultivation time is 13~18 days, or 14~16 days.

[0041] The rough-skinned Pleurotus ostreatus obtained by the domestication and cultivation method described in this invention has a short culture time and a high crude protein content in the fruiting body, making it a high-quality edible fungus worthy of promotion.

[0042] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0043] Example 1 The isolation, purification, and identification of wild-type Pleurotus ostreatus strains were carried out as follows: A wild *Pleurotus ostreatus* strain was collected in August 2025 from Zhangguangcailing Laojieji Forest Farm, Shangzhi City, Heilongjiang Province (44°54'N, 127°57'E), specimen number ZGCL25-8-11-11. Figure 1 As shown.

[0044] 1) Obtaining the strain using tissue isolation: A small piece of tissue was cut from the junction of the stipe and cap of wild-collected *Pleurotus ostreatus* fruiting bodies using a sterile knife. This tissue was inoculated into PDA medium containing antibiotics (100 U / mL penicillin and 100 μg / mL streptomycin) and incubated at 25°C in the dark for 3 days. Mycelial germination was observed; the mycelial growth was uniform, dense, and odorless. Pure mycelia germinated on PDA medium containing antibiotics.

[0045] 2) Obtaining pure mycelial strain using the hyphal tip purification method: Select culture dishes with vigorous, uniform, dense, and odorless hyphae free from mold contamination. In a clean bench, use an inoculation needle to pick healthy hyphae (the tips) from the uncontaminated area and transfer them to fresh PDA medium. Incubate at 20°C in the dark for 3-7 days, observing hyphal growth daily to obtain pure mycelial strains. Figure 2 As shown.

[0046] 3) Morphological identification: Morphological identification was performed according to the "Illustrated Guide to Macrofungi Resources of China" and the "Color Atlas of Wild Macrofungi", focusing on macroscopic morphology and microscopic structure. The structure of the fruiting body cap, flesh color, and attachment method were observed and measured, and hyphae and spores were observed and measured under a microscope.

[0047] Macroscopic morphological observations revealed that the fruiting bodies grow on fallen, decaying wood in forests. The caps are fan-shaped to semi-circular, with smooth surfaces and a uniform light beige to light grayish-brown color. The cap edges are thin and wavy. The gills are decurrent, densely and regularly arranged, and light in color. The stipe is short and thick, with a white base (as shown in the image). Figure 1 (As shown). The above characteristics are basically consistent with the description of Pleurotus ostreatus in the "Illustrated Handbook of Macrofungi Resources of China".

[0048] Microscopic morphological observation results: In PDA medium, the mycelium was white and dense, with well-developed aerial hyphae, and the mycelium grew rapidly and vigorously. Optical microscopy revealed that the mycelium had distinct clamp connections, and the hyphal diameter was approximately 3.0–5.1 μm. The basidiospores were elliptical, smooth and colorless, with a spore size of (3.52–7.51) × (2.78–3.45) μm, consistent with the typical spore morphology of the genus *Pleurotus*.

[0049] 3) Molecular biological identification: Total DNA was extracted from mycelium using a DNA extraction kit (Tiangen Biotech, DP320). The ITS fragment was amplified using primers ITS1 5'-TCCGTAGGTGAACCTGCGG-3' (SEQ ID NO:1) and ITS4 5'-TCCTCCGCTTATTGATATGC-3' (SEQ ID NO:2). The amplified products were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing to obtain the ITS sequence. The sequence was then subjected to BLAST alignment analysis using the NCBI database, and a phylogenetic tree was constructed using Mega 11.0 based on the neighbor-joining method to determine its phylogenetic position.

[0050] Molecular biological identification results: The ITS amplification product of ZGCL25-8-11-11 appeared as a single bright band of approximately 700 bp after 1.0% agarose gel electrophoresis. Sequencing results revealed that the ITS sequence length of ZGCL25-8-11-11 was 684 bp. BLAST alignment showed 99.70%–100% homology with the ITS of Pleurotus ostreatus. Auricularia heimuer For outgroups, construct a phylogenetic tree based on the adjacency method, such as Figure 4 As shown.

[0051] Depend on Figure 3 It can be seen that ZGCL25-8-11-11 and 2 P. ostreatus On the same branch, the branch support rate is 99%.

[0052] Based on the above identification results, this strain can be clearly identified as Pleurotus ostreatus, and it is named ZGCL25-8-11-11 and deposited at the China General Microbiological Culture Collection Center (CGMCC).

[0053] Example 2 The mycelial growth rate of wild Pleurotus ostreatus ZGCL25-8-11-11 was determined using the following steps: The pure mycelial spawn of wild Pleurotus ostreatus ZGCL25-8-11-11 and Pleurotus ostreatus var. 650 from Example 1 were transferred to PDA agar plates for activation. After the plates were almost fully covered, the colonies were punched with a 7 mm punch and transferred to another agar plate. The wild Pleurotus ostreatus ZGCL25-8-11-11 and Pleurotus ostreatus var. 650 were placed in incubators at 28℃ and 26℃ respectively. The streak method was used to draw lines every 4 days. The mycelial growth rate was measured on the 8th day. Three biological replicates were set up. Mycelial growth rate (mm / d) = (colon diameter on the 8th day - colony diameter on the 4th day) / time.

[0054] The results showed that the average daily growth rate of mycelium of wild Pleurotus ostreatus ZGCL25-8-11-11 reached 1.05 cm / d, while that of Pleurotus ostreatus var. 650 reached 0.96 cm / d. This indicates that the mycelium growth rate of wild Pleurotus ostreatus ZGCL25-8-11-11 was faster than that of ordinary strains.

[0055] Example 3 The high-temperature adaptability test of wild rough-skinned Pleurotus ostreatus ZGCL25-8-11-11 was conducted as follows: Wild Pleurotus ostreatus ZGCL25-8-11-11 was inoculated into a mother culture medium containing 200 g / L potato, 20 g / L glucose, 1.5 g / L magnesium sulfate, and 2.5 g / L potassium dihydrogen phosphate at a natural pH. The culture was incubated at 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, and 38℃. The results showed that the mycelium could grow normally at temperatures ranging from 15℃ to 38℃, with the optimal growth temperature being 25℃ to 30℃.

[0056] The mother culture of Pleurotus ostreatus ZGCL25-8-11-11 was inoculated into the original culture medium and cultured at 25~30℃. When the mycelium covered the culture bag, the original culture was obtained. The original culture medium formula is: 65% sawdust, 15% corn cob, 18% wheat bran, 0.5% quicklime and 0.5% gypsum.

[0057] The original strain of Pleurotus ostreatus ZGCL25-8-11-11 was inoculated into the cultivation substrate, which consisted of 80% corn cob, 16% wheat bran, 3% rice bran, 0.5% quicklime, and 0.5% gypsum. The substrate was incubated in the dark at a temperature of 25-30℃ and a humidity of 45% for 30 days.

[0058] The results showed that the fruiting bodies could develop normally at 25-30℃, without deformities at high temperatures, and the fruiting bodies were blackish-gray in color.

[0059] Example 4 The domestication and cultivation of wild rough-skinned Pleurotus ostreatus ZGCL25-8-11-11 follows these steps: 1) Mother culture preparation: Inoculate the purified strain from Example 1 into the mother culture medium and incubate in the dark at 25°C for 8 days. Once the mycelium has rapidly grown to cover the test tube, the mother culture can be obtained. Mother culture medium formula: 200 g / L potato, 25 g / L glucose, 1.5 g / L magnesium sulfate, 3.0 g / L potassium dihydrogen phosphate, natural pH.

[0060] 2) Preparation of primary culture: After packaging the primary culture medium according to the formula, autoclave at 121℃ for 2 hours. After cooling the medium to 35℃, inoculate with the mother culture and incubate in the dark at 25℃ for 30 days. Once the mycelium has fully colonized the culture bag, the primary culture is obtained. Figure 4 As shown; Original seed cultivation substrate formula: 60% sawdust, 20% corn cob, 18% wheat bran, 1% quicklime, and 1% gypsum.

[0061] 3) Preparation of spawn: Pile the substrate into heaps and allow it to decompose at 25℃ for 15 days, turning it 1-2 times during this period. After decomposition, bag the substrate and sterilize it at 100℃ under normal pressure for 10 hours. After the substrate cools to 35℃, inoculate it with the original spawn and transfer it to a mycelium cultivation room. The mycelium cultivation temperature is 25℃, and the humidity is 40%-50%. Cultivate in the dark for 30 days. Once the mycelium has fully colonized the bags, the spawn is obtained. Figure 5 As shown; Cultivation substrate formula: 80% corn cob, 15% wheat bran, 3% rice bran, 1% quicklime, and 1% gypsum.

[0062] 4) Cultivation and Fruiting Management Techniques: Place the spawn in the fruiting room, control the temperature at 25~32℃, the relative humidity at 85%~90%, ventilate twice daily, and maintain a light intensity of 400~800 lux. Harvesting can begin after 15 days of cultivation. The fruiting conditions should be as follows: Figure 6 As shown, the sub-entities are as follows Figure 7 As shown.

[0063] Mushroom yield: The yield of a single flush of mushrooms per bag is 452 g, with a bioconversion rate of 85%.

[0064] Example 5 The nutrient content of the cultivated *Pleurotus ostreatus* seed bodies (ZGCL25-8-11-11) and *Pleurotus ostreatus* fruiting bodies (No. 1) obtained in Example 4 was determined. The nutrient content included total sugar, crude protein, crude fiber, and crude fat. The total sugar content was determined according to the method in *NY / T1676*, the crude protein content according to the method in *GB 5009.5*, the crude fiber content according to the method in *GB / T 5009.10*, and the crude fat content according to the method in *GB5009.6*. The results are shown in Table 1 and... Figure 8 As shown.

[0065] Table 1. Results of nutrient content determination in the fruiting bodies of *Pleurotus ostreatus* ZGCL25-8-11-11 cultivated seeds and *Pleurotus ostreatus* No. 1.

[0066] From Table 1 and Figure 8 The results show that the crude protein content of the cultivated seed bodies of Pleurotus ostreatus ZGCL25-8-11-11 is significantly higher than that of Pleurotus ostreatus No. 1.

[0067] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A wild, rough-skinned pleiocarpa ( Pleurotus ostreatus ZGCL25-8-11-11, accession number CGMCCNo.42099.

2. The pure mycelial strain of wild Pleurotus ostreatus ZGCL25-8-11-11 as described in claim 1 is a strain obtained by transferring the germinating mycelia of wild Pleurotus ostreatus ZGCL25-8-11-11 as described in claim 1 to a culture medium.

3. The use of the wild Pleurotus ostreatus ZGCL25-8-11-11 as described in claim 1 or the pure mycelial strain as described in claim 2 in the cultivation of at least one of the Pleurotus ostreatus mother strain, the original strain of Pleurotus ostreatus, and the cultivated strain of Pleurotus ostreatus.

4. A rough-skinned pleurotus species, characterized in that, The method involves inoculating the pure mycelial strain described in claim 2 into a mother culture medium and then incubating it in the dark for the first time. The mother culture medium comprises the following components at the following concentrations: potato 190-210 g / L, glucose 20-30 g / L, magnesium sulfate 1.0-2.0 g / L, potassium dihydrogen phosphate 2.5-3.5 g / L, and natural pH.

5. A rough-skinned pleurotus species, characterized in that, The method involves inoculating the rough-skinned Pleurotus ostreatus mother seed as described in claim 4 into the original seed cultivation material and then incubating it in the dark for a second time. The original seed cultivation material comprises the following components in weight percentage: 55%~65% sawdust, 15%~25% corn cob, 16%~20% wheat bran, 0.5%~1.5% quicklime, and 0.5%~1.5% gypsum.

6. A rough-skinned pleurotus ostreatus cultivar, characterized in that, The method involves inoculating the original strain of Pleurotus ostreatus as described in claim 5 into a well-rotted cultivation substrate for mycorrhizal cultivation. The cultivation substrate comprises the following components in weight percentage: 75%~85% corn cob, 10~20% wheat bran, 2.5%~3.5% rice bran, 0.5%~1.5% quicklime, and 0.5%~1% gypsum.

7. The rough-skinned Pleurotus ostreatus cultivar according to claim 6, characterized in that, The bacteria are cultured in the dark, at a temperature of 20-25°C, at a humidity of 40%-50%, and for a duration of 25-30 days.

8. A method for the domestication and cultivation of wild rough-skinned Pleurotus ostreatus ZGCL25-8-11-11, characterized in that, Includes the following steps: The pure mycelial strain described in claim 2 is inoculated into the mother culture medium and subjected to a first dark culture to obtain the mother culture; the mother culture medium comprises the following components at the following concentrations: potato 190~210 g / L, glucose 20~30 g / L, magnesium sulfate 1.0~2.0 g / L, potassium dihydrogen phosphate 2.5~3.5 g / L, and natural pH. The mother seed is inoculated into the original seed culture medium for a second dark culture to obtain the original seed of Pleurotus ostreatus; the original seed culture medium includes the following components by weight percentage: sawdust 55%~65%, corn cob 15%~25%, wheat bran 16%~20%, quicklime 0.5%~1.5% and gypsum 0.5%~1.5%. The original cultivar of Pleurotus ostreatus was inoculated into well-rotted cultivation substrate to obtain the cultivar of Pleurotus ostreatus. The cultivation substrate comprises the following components by weight percentage: 75%~85% corn cob, 10~20% wheat bran, 2.5%~3.5% rice bran, 0.5%~1.5% quicklime, and 0.5%~1% gypsum. The rough-skinned pleurotus ostreatus cultivar was subjected to fruiting culture to obtain rough-skinned pleurotus ostreatus fruiting bodies.

9. The domestication and cultivation method according to claim 8, characterized in that, The temperature of the first dark incubation is 25~27℃, and the duration of the first dark incubation is 6~9 days; The temperature for the second dark culture was 20-25°C, and the duration of the second dark culture was 6-9 days.

10. The domestication and cultivation method according to claim 9, characterized in that, The bacterial culture is carried out in the dark, the temperature is 20~25℃, the humidity is 40%~50%, and the culture time is 25~30 days. The temperature for fruiting cultivation is 20~28℃, the relative humidity of the air for fruiting cultivation is 85%~90%, the light intensity for fruiting cultivation is 400~800 lux, and the fruiting cultivation time is 13~18 days.

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