Soybean gene GmCIB1and gene GmCRY2 and application thereof for regulating and controlling flowering and aging

A genetic and aging technology, applied to the soybean GmCIB1 gene and GmCRY2 gene and their application fields, can solve the problem of changing the sensitivity of soybean flowering to photoperiod

Inactive Publication Date: 2017-08-18
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0063] Whether overexpression or RNAi knockout of the GmCRY gene in soybean can alter the sensitivity of soybean flowering to photoperiod has not been reported before this study

Method used

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  • Soybean gene GmCIB1and gene GmCRY2 and application thereof for regulating and controlling flowering and aging
  • Soybean gene GmCIB1and gene GmCRY2 and application thereof for regulating and controlling flowering and aging
  • Soybean gene GmCIB1and gene GmCRY2 and application thereof for regulating and controlling flowering and aging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] Example 1. Cloning and expression pattern analysis of soybean CIB family genes

[0152] In 2008, the first blue-light-specific interaction protein with cryptochrome CRY2 was screened in Arabidopsis thaliana by yeast two-hybrid, named CIB1 (CRY-interacting bHLH1). CIB1 is a member of the bHLH family. In yeast cells, the interaction between CIB1 and CRY2 is specific to the wavelength of blue light, and the strength of the interaction increases with the increase of blue light intensity and illumination time. The structure-function study of CIB1 shows that its N-terminal domain can perform the function of interacting with proteins; while the C-terminal domain containing the bHLH motif participates in the interaction with DNA, thereby regulating target genes through cis-acting elements. In Arabidopsis, CIB1 increases the transcription level of FT by combining with the E-box (CANNTG) of the FT promoter region, thereby initiating plant flowering.

[0153] In this study, nine...

Embodiment 2

[0377] Example 2, Functional Research of GmCRY1&2 and GmCIB1 Regulating Flowering and Senescence

[0378] In this study, we will study the interaction between GmCRYs and GmCIB1 protein by yeast two-hybrid, BiFC and Co-IP methods; further obtain soybean genetically transformed lines with overexpression or RNAi knockout of GmCRY and GmCIB1 genes, and combine the phenotypes of transgenic offspring Analysis and ChIP experiments were used to find the downstream target genes of GmCIB1, so as to analyze the GmCIB signaling pathway through which GmCRY transmits blue light signals in soybeans, laying a foundation for in-depth research on soybean photoperiod and leaf senescence regulation and germplasm innovation.

[0379] 1. Materials and methods

[0380] 1.1 Materials

[0381] 1.1.1 Plant material

[0382] The soybean variety is Kennong 18, and the tobacco variety is Nicotiana Benthamiana.

[0383] 1.1.2 Strains and plasmids

[0384] The strains of Agrobacterium and Escherichia co...

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Abstract

The invention discloses a soybean gene GmCIB1 and a gene GmCRY2 and application thereof for regulating and controlling flowering and aging, and particularly relates to a soybean gene GmCIB1 and a gene GmCRY2 with sequences shown in SEQ ID NO: 1 or 3, a DNA (deoxyribonucleic acid) molecule containing the genes, a carrier, protein encoded by the gene and related transformation cells and transgenic plants. The invention also relates to application of the genes in regulating and controlling plant leaf aging and flowering, and a method for culturing related transgenic plants.

Description

[0001] This application is a divisional application of an invention patent application with an application date of October 12, 2013, an application number of 201310490925.4, and an invention title of "soybean GmCIB1 gene and GmCRY2 gene and their functions in regulating flowering and aging". technical field [0002] The invention relates to the field of biotechnology, in particular to soybean GmCIB1 gene and GmCRY2 gene and application thereof. Background technique [0003] In nature, the study of plant senescence has become one of the important issues in plant life science research. Thimann et al. proposed in 1978 that plant senescence is a series of decline processes during growth and development (Thimann, 1978). Ten years later, Roach proposed that senescence is a physiological process in which the survival and reproductive ability of plants gradually decreases with the growth of age (Roach et al., 1993). In 1997, Nooden perfected this concept and proposed that senescenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
CPCC07K14/415C12N15/8266C12N15/827
Inventor 李宏宇孟颖颖刘斌赵涛刘军林辰涛
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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