Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker and application thereof

A molecular marker, rice technology, applied in the field of molecular biology, to achieve the effect of saving time, high accuracy and low cost

Active Publication Date: 2018-06-29
SHENZHEN HUADA GENE INST +3
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on this aspect at home and abroad. If the genes related to the cadmium repellency trait can be located and the DNA molecular markers closely linked to it can be developed, the molecular marker-assisted breeding technology can be used to guide the rice breeding work, which will greatly improve the quality of the rice. Improving the breeding level and breeding efficiency of cadmium-resistant rice is of great significance to rice food security
However, at this stage, the genes related to cadmium rejection and the DNA molecular markers closely linked to the genes are still to be discovered.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker and application thereof
  • Molecular marker and application thereof
  • Molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1: Construction of rice F2 generation segregation population and F2 generation grain cadmium content phenotype data collection

[0072] The male parent is the indica-type conventional rice variety 9311, which was planted in cadmium-contaminated soil with a cadmium soil background value of 4.3mg / kg. Varieties with high accumulation of cadmium do not have cadmium repelling traits.

[0073] The female parent is the indica-type photothermo-sensitive male sterile line T91S, which belongs to the indica-type dual-purpose genic male sterile line. Planted in cadmium-contaminated soil with a cadmium soil background value of 4.3mg / kg, the cadmium content in the grain is 0.024mg / kg, which is lower than the national standard of 0.2mg / kg.

[0074] The male parent and the female parent were crossed to obtain F1, and the F1 generation was self-crossed to generate the F2 generation population, with a total of 209 individual plants. The F2 population was planted in cadmium-cont...

Embodiment 2

[0080] Example 2: Extraction of Genomic DNA

[0081] The genomic DNA of the parents, F1 generation, and F2 generation individuals in Example 1 were extracted respectively with the improved CTAB method, and the specific methods were as follows:

[0082] 1) Sampling: Take about 1g of young leaves and place them in a 2ml centrifuge tube.

[0083] 2) Freeze-drying of the sample: Take the good sample and grind the beads, open the cap of the tube and put it into a vacuum freeze-dryer, and continue to freeze-dry at -50°C for more than 12 hours (generally freeze-dry overnight).

[0084] 3) Sample grinding: the freeze-dried sample is placed in a high-throughput mechanical grinder, and the grinding program is started to pulverize the sample. The mechanical grinder program takes 5 minutes to run with a throughput of 96 samples / 5 minutes.

[0085] 4) After the sample is sufficiently ground, add CTAB extract solution preheated at 65°C for 1 hour into the tube, mix well, and place in a wa...

Embodiment 3

[0092] Example 3: Gene Mapping and Molecular Marker Development

[0093] (1) Genetic map construction

[0094] For the genomic DNA of the F2 individuals obtained in Example 2, the RAD-seq-based genotyping technology was used to genotype the individuals of the F2 population to obtain genotype data of the F2 population.

[0095] Use MapMaker 3.0 software (Constructing genetic maps with MAPMAKER / EXP 3.0, SLincoln, M Daly, E Lander-Cambridge, MA: Whitehead Institute, 1992, the full text of which is incorporated herein by reference) to draw a genetic linkage map to obtain a genetic linkage map .

[0096] (2) Gene mapping

[0097] According to the phenotype data of the F2 population obtained in Example 1, combined with the population genotype and genetic linkage map, QTL mapping was carried out by WinQTLCart 2.5 software.

[0098] Since the parents have obvious differences in cadmium repelling traits (the male parent does not have the cadmium repelling trait, the female parent ha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker and application thereof. The molecular marker shows insertion / deletion length polymorphism of a nucleotide sequence shown as SEQ ID NO:3. The molecular marker disclosed by the invention is tightly associated with cadmium repelling character of rice and can be effectively used for gene mapping and detection of the cadmium repelling character of the rice aswell as assistant breeding of the molecular marker of the rice.

Description

[0001] priority information [0002] This application claims priority and rights to the patent application No. CN201611177123.8 filed with the State Intellectual Property Office of China on December 19, 2016, which is hereby incorporated by reference in its entirety. technical field [0003] The invention relates to the field of molecular biology, in particular to molecular markers and applications thereof. Background technique [0004] Cadmium (Cd) is considered one of the most toxic environmental pollutants. On the one hand, Cd in soil comes from natural conditions such as mineral weathering, iron-manganese co-precipitation, atmospheric dry and wet deposition, and on the other hand, it comes from human factors such as the application of chemical fertilizers, pesticides and soil improvers, and sewage irrigation. [1] . According to incomplete statistics, the area polluted by heavy metals Cd and Pb in my country's farmland has reached 20,000 hectares, and the annual product...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张耕耘倪雪梅侯军亮
Owner SHENZHEN HUADA GENE INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com