Rapid screening method for drought-enduring variety of rice at seedling stage

A screening method, rice seedling technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as increased water viscosity, hypoxia, single drought treatment method, etc., to save time and money, and accurate soil drought , the effect of accurate experimental results

Inactive Publication Date: 2013-01-09
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

What kind of drought treatment method to use to study the damage of drought stress to plants, researchers have made many attempts, the most widely used are soil drought treatment and PEG (polyethylene glycol) osmotic stress simulation drought treatment, but both methods exist There are some problems, such as uncertainty in the treatment time of soil drought stress treatment, researchers cannot clearly define the start time of drought treatment, and there are also shortcomings such as the inability to accurately adjust the water in the soil; PEG osmotic stress simulates drought to solve the problem of soil drought The above disadvantages can precisely control the start time of drought stress and the intensity of drought stress, but this simulation method is a method derived from hydroponics, and there are common problems of hydroponics methods, that is, the root system grows in the water, resulting in root hypoxia, while PEG After being dissolved in water, the viscosity of water is greatly increased, which leads to more serious root hypoxia
[0003] Due to the shortcomings of using a single drought treatment method, it cannot meet the needs of researchers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A: Soil drought treatment method:

[0031] (1) Take river sand and soil and dry them at 110-130°C for 4-5 hours to dry and weigh them;

[0032] (2) Take 4 petri dishes, and each petri dish is filled with 120g of sand and soil mixture according to the weight ratio of river sand:soil of 4:1;

[0033] (3) Choose healthy seeds with plump grains, disinfect them with 10% sodium hypochlorite, wash them with water for 5 times, germinate them at 35°C, and sow them in petri dishes, one variety per petri dish, 100 grains, and the four varieties are rice " Tianyou 998", rice "Jiaxing 8", rice "Jijing 83", rice "Biaoyou 2";

[0034] (4) Weigh the Petri dishes after sowing the seeds, and record the weights as 607g, 643g, 611g, and 626g respectively, and then add 30g of water respectively;

[0035] (5) Culture the petri dish in an artificial climate box with a light intensity of 100 μmol / m 2 s , temperature 30 ℃ , humidity 80 % , light for 16 hours a day , weigh each petri dish eve...

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Abstract

The invention discloses a rapid screening method for drought-enduring variety of rice at seedling stage, comprising the following steps that: after soil drought processing and drought processing simulated by polyethylene glycol (PEG), variety with strong drought tolerance in two processing methods is screened out. By simultaneously performing soil drought and drought simulated by PEG and conjointly analyzing the result, the defects of two drought processing methods can be simultaneously avoided and the advantages of two methods can be exploited to the full. Thus, the experimental result is accurate and the results of the studies of breeding, group construction, gene mapping and the like of the drought-enduring variety and drought-sensitive variety which are screened out are more credible, and the time and the expenditure of researchers are saved.

Description

technical field [0001] The invention mainly relates to a rice screening method, in particular to a rapid screening method for rice seedling drought-tolerant varieties. Background technique [0002] Water shortage is a global problem currently restricting agricultural production. About 43% of the world's arable land is in arid and semi-arid areas. Drought stress seriously affects the growth and development of plants, resulting in crop yield reduction. my country is a water-poor country, and the contradiction between supply and demand of water resources is very sharp. In recent years, water shortage will be one of the most serious problems facing our country. Moreover, in agricultural production, frequent droughts often occur, causing huge losses to food production. The annual loss of food production caused by drought in the world is almost equal to the sum of losses caused by all other environmental factors. Therefore, research on the response of crops to drought stress...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06
Inventor 李泽福马廷臣夏加发王元垒
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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