Common camellia oleifera SSR molecular marker primers and marking method and application thereof

A technology of molecular markers and Camellia oleifera, which is applied in biochemical equipment and methods, microbe measurement/testing, DNA/RNA fragments, etc., can solve the problems of rapid cell division

Active Publication Date: 2019-08-20
QINZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research on the transcriptome and SSR markers of common camellia oleifera root. Camellia oleifera root grows fast and cells divide rapidly. The development and transfer of transcriptome sequencing and SSR mol

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  • Common camellia oleifera SSR molecular marker primers and marking method and application thereof
  • Common camellia oleifera SSR molecular marker primers and marking method and application thereof
  • Common camellia oleifera SSR molecular marker primers and marking method and application thereof

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Embodiment

[0034] 1. Experimental method:

[0035] 1. RNA extraction method of sample camellia oleifera:

[0036] 1) Experimental materials for transcriptome sequencing: the experimental Camellia oleifera variety is the common Camellia oleifera variety "Cenruan No. 3".

[0037] 2) Sample processing: three key time nodes in the rooting process of "Cenruan No. 3" in the tissue culture process: before rooting (CK group), 1 day after rooting (T1 group), and 14 days after rooting That is, [appear root point] (T2 group), 50 days after inserting and rooting [root growth stage] (T3 group), after obtaining the root material, use ddH 2 After rinsing the agarose, put it into liquid nitrogen immediately, and wait for the RNA to be extracted.

[0038] 3) Extraction and sequencing method: Use RNAiso for polysaccharide-rich Plant Tissue to extract RNA from all samples, and mix equal amounts of CK, T1, T2 and T3 to construct a cDNA library for Illumina HiSeq2000 sequencing.

[0039] 2. Experimental m...

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Abstract

The present invention relates to the technical field of molecular marking and particularly relates to common camellia oleifera SSR molecular marker primers and a marking method and an application thereof. The common camellia oleifera roots are subjected to RNA extraction, sequencing and assembly, MISA is used to search SSR sites, corresponding primers are designed, camellia oleifera genome DNA isused as a material, the designed SSR primers are validated, and the corresponding primers are obtained from the designed 6,738 pairs of the primers. The primers show polymorphism, can successfully distinguish the camellia oleifera materials of different varieties, provide the new molecular markers for molecular marking auxiliary breeding of the camellia oleifera, and provide new ideas for future researches on genetic breeding, genome mapping, gene mapping, species genetic relationship identification, etc.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of molecular markers, in particular to a common Camellia oleifera SSR molecular marker primer and a marker method and application. [0003] 【Background technique】 [0004] Ordinary Camellia oleifera is the woody oil plant with the most extensive planting area and the highest total output among Camellia oleifera species, and is often used to extract high-quality edible camellia oil; in addition, the oil-extracting residue can be further processed for use in chemical, textile, and pesticide industries raw materials. Because its unsaturated fatty acid content is as high as more than 90%, camellia oil is a kind of high-quality edible oil, which has been promoted as a healthy high-grade edible oil by the Food and Agriculture Organization of the United Nations. With the improvement of living standards, people's requirements for edible oil also increase. Camellia oil has become in short supply and it is d...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 王鹏良杨利平王华宇
Owner QINZHOU UNIV
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