New method for mapping corn S-type cytoplasm male sterility fertility restoring gene Rf3

Abstract

The invention belongs to the technical field of corn gene mapping, in particular to a new method for mapping a corn S-type cytoplasm male sterility fertility restoring gene Rf3. According to a conventional method for mapping a qualitative character gene, a backcross or selfing population is constructed, the gene type of an individual is inferred through phenotype identification, molecular marker detection is performed, and the position of the gene is determined through two-point and three-point tests. When the Rf3 gene is mapped with the conventional method, the phenotype identification is affected by human and environmental factors easily, the workload is large and the size of the population to be mapped is limited. The mapping method aims to overcome the defects of the conventional method for mapping the Rf3 gene and does not need the phenotype identification. The new method is characterized in that due to the fact that the gene type of the utilized population to be mapped is already known, the phenotype does not need to be identified, a large population can be constructed, and enough recombined individuals can be found in one generation to fine map the Rf3 gene. The method for mapping the Rf3 gene is effective and promotes three-line sterility seed production by the utilization of the Rf3 gene in production.

Description

technical field [0001] The invention belongs to the technical field of maize gene positioning, and in particular relates to a new method for locating the S-type cytoplasmic male sterility restoration gene Rf3 of maize. Background technique [0002] Corn is not only one of the three major food crops in the world, but also an important feed and industrial raw material crop. From the perspective of future development, corn will be the crop with the fastest growing demand in the world, and tapping the production potential of corn is the key to meeting this demand. The improvement of maize yield is mainly achieved by planting hybrids. Therefore, the preparation of hybrids with high yield potential and high purity is the key link to ensure high maize yield. In the process of producing hybrids, it is necessary to detassell the female parent. In the process of intensive production of hybrid seeds, the workload of female parent emasculation is large, the cost of seeds is high, and o...

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Problems solved by technology

[0014] The present invention aims at the disadvantages that the existing maize Rf3 gene localization phenotype identification is easily affected by man-made and environmental factors, and the workload is large to limit the size of the localization population, and proposes an Rf3 gene localization method that does not require phenotypic identification

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